Circ004463 promotes fibroblast proliferation and collagen I synthesis by sponging miR-23b and regulating CADM3/MAP4K4 via activation of AKT/ERK pathways

Zou, Qin; Wang, Xue; Yuan, Rong; Gong, Zhengyin; Luo, Chenggang; Xiong, Ying; Jiang, Yanzhi

doi:10.1016/j.ijbiomac.2022.12.029

Cited by 6 publications

(6 citation statements)

References 25 publications

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“…In our pervious report, the skin of CH pig was thicker than that of Large White (LW) pigs (8.5 mm vs. 3.0 mm) (Zou et al, 2022), which was consistent with the current data (Figure 6A and Figure 6—figure supplement 1A). Curiously, the pattern of heterogeneity in skin cell for different skin anatomic sites is whether also exist in different breed.…”

Section: Resultssupporting

confidence: 92%

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Single-cell transcriptome analysis on the anatomic positional heterogeneity of pig skin

Zou

Yuan

Wang

et al. 2023

Preprint

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Different anatomic locations of the body skin dermis come from different origins, and its positional hereditary information can be maintained in adults, while highly resolvable cellular specialization is less well characterized in different anatomical regions. Pig is regarded as excellent model for human research in view of its similar physiology to human. In this study, we performed single-cell RNA sequencing of six different anatomical skin regions from the Chenghua pig with superior skin thickness traits. We obtained 215,274 cells, representing seven cell types, among which we primarily characterized the heterogeneity of smooth muscle cells, endothelial cells and fibroblasts. We identified several phenotypes of smooth muscle cell and endothelial cell and presented genes expression of pathways such as the immune response in different skin regions. By comparing differentially expressed fibroblast genes among different skin regions, we considered TNN, COL11A1, and INHBA as candidate genes for facilitating ECM accumulation. These findings of heterogeneity in the main three cell types from different anatomic skin sites will contribute to a better understanding of hereditary information and places the potential focus on skin generation, transmission and transplantation, paving the foundation for human skin priming.

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Section: Resultssupporting

confidence: 92%

“…Then, the sections were cut at a thickness of 5 µm using a microtome. The dermal thickness was determined using CaseViewer software according to a previous method after hematoxylin-eosin staining (Zou et al, 2022). The mean values and standard deviations were calculated.…”

Section: Methodsmentioning

confidence: 99%

Single-cell transcriptome analysis on the anatomic positional heterogeneity of pig skin

Zou

Yuan

Wang

et al. 2023

Preprint

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“…Our previous studies discovered that the two pig populations, namely CH and LW pigs with a significant difference in skin thickness, showed gene expression changes at whole transcriptome levels in skin tissues and small extracellular vesicles derived from skin FBs ( Zou et al, 2022 ; Zou et al, 2023 ). Curiously, the pattern of heterogeneity in skin cells is whether it also exists in the two pig populations and contributes to the difference of skin thickness.…”

Section: Resultsmentioning

confidence: 99%

“…However, the single-cell transcriptional diversity of different anatomical skin regions has not been understood. The CH pig, a novel Chinese indigenous breed with superior skin thickness, is considered as a potential model animal for researching mammalian skin biology ( Zou et al, 2022 ; Zou et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

“…The pig is used as a model animal to research human skin biology because of its similar pathological and physiological skin attributes to those of human skin ( Khiao In et al, 2019 ). The Chenghua (CH) pig, a novel Chinese indigenous population with superior skin thickness and strong FBs activity ( Zou et al, 2022 ; Zou et al, 2023 ), is considered as a potential model animal for researching mammalian skin biology. Here, to reveal the anatomical positional heterogeneity of the skin, single-cell RNA sequencing was performed on pig skin tissues from six anatomical regions of CH pigs and the back site of large white (LW) pigs.…”

Section: Introductionmentioning

confidence: 99%

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A single-cell transcriptome atlas of pig skin characterizes anatomical positional heterogeneity

Zou

Yuan

Wang

et al. 2023

eLife

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Different anatomical locations of the body skin show differences in their gene expression patterns depending on different origins, and the inherent heterogeneous information can be maintained in adults. However, highly resolvable cellular specialization is less well characterized in different anatomical regions of the skin. Pig is regarded as an excellent model animal for human skin research in view of its similar physiology to human. In this study, single-cell RNA sequencing was performed on pig skin tissues from six different anatomical regions of Chenghua (CH) pigs, with a superior skin thickness trait, and the back site of large white (LW) pigs. We obtained 233,715 cells, representing seven cell types, among which we primarily characterized the heterogeneity of the top three cell types, including smooth muscle cells (SMCs), endothelial cells (ECs), and fibroblasts (FBs). Then, we further identified several subtypes of SMCs, ECs, and FBs, and discovered the expression patterns of site-specific genes involved in some important pathways such as the immune response and extracellular matrix (ECM) synthesis in different anatomical regions. By comparing differentially expressed genes of skin FBs among different anatomical regions, we considered TNN, COL11A1, and INHBA as candidate genes for facilitating ECM accumulation. These findings of heterogeneity in the main skin cell types from different anatomical sites will contribute to a better understanding of inherent skin information and place the potential focus on skin generation, transmission, and transplantation, paving the foundation for human skin priming.

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Comprehensive transcriptional analysis of early dorsal skin development in pigs

zhang,

Zhang,

et al. 2024

Gene

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Circ004463 promotes fibroblast proliferation and collagen I synthesis by sponging miR-23b and regulating CADM3/MAP4K4 via activation of AKT/ERK pathways

Cited by 6 publications

References 25 publications

Single-cell transcriptome analysis on the anatomic positional heterogeneity of pig skin

Single-cell transcriptome analysis on the anatomic positional heterogeneity of pig skin

A single-cell transcriptome atlas of pig skin characterizes anatomical positional heterogeneity

Comprehensive transcriptional analysis of early dorsal skin development in pigs

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