Circ
            <scp>HIPK</scp>
            3 sponges miR‐558 to suppress heparanase expression in bladder cancer cells

Li, Yawei; Zheng, Fuxin; Xiao, Xingyuan; Xie, Fei; Tang, Dan; Huang, Chao; Liu, Dong; Wang, Miao; Wang, Liang; Zeng, Fuqing; Jiang, Guosong

doi:10.15252/embr.201643581

Cited by 475 publications

(475 citation statements)

References 46 publications

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“…CircHIPK2 increased SIGMAR1 expression by suppressing miR-124-2HG activity [22]. In bladder cancer, circHIPK3 affected the expression of heparanase by competitively binding to miR-558 [23]; circRNA MYLK and lncRNA H19 increase the expression of DNMT3, VEGFA, and ITGB1 by suppressing miR-29a-3P activity [24].…”

Section: Discussionmentioning

confidence: 99%

The CircRNA-ACAP2/Hsa-miR-21-5p/ Tiam1 Regulatory Feedback Circuit Affects the Proliferation, Migration, and Invasion of Colon Cancer SW480 Cells

Han

et al. 2018

Cell Physiol Biochem

108

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Background/Aims: Circular RNAs (circRNAs), a type of RNA that is widely expressed in human cells, have essential roles in the development and progression of cancer. CircRNAs contain microRNA (miRNA) binding sites and can function as miRNA sponges to regulate gene expression by removing the inhibitory effect of an miRNA on its target gene. Methods: We used the bioinformatics software TargetScan and miRanda to predict circRNA-miRNA and miRNAi-Mrna interactions. Rate of inhibiting of proliferation was measured using a WST-8 cell proliferation assay. Clone formation ability was assessed with a clone formation inhibition test. Cell invasion and migration capacity was evaluated by performing a Transwell assay. Relative gene expression was assessed using quantitative real-time polymerase chain reaction and relative protein expression levels were determined with western blotting. circRNA and miRNA interaction was confirmed by dual-luciferase reporter and RNA-pull down assays. Results: In the present study, the miRNA hsa-miR-21-5p was a target of circRNA-ACAP2, and T lymphoma invasion and metastasis protein 1 (Tiam1) was identified as a target gene of hsa-miR-21-5p. CircRNA-ACAP2 and Tiam1 were shown to be highly expressed in colon cancer tissue and colon cancer SW480 cells, but miR-21-5p was expressed at a low level. SW480 cell proliferation was suppressed when the expression of circRNA-ACAP2 and Tiam1 was decreased and the expression of miR-21-5p was increased in vivo and in vitro. SW480 cell migration and invasion were also inhibited under the same circumstance. The circRNA-ACAP2 interaction regulated the expression of miR-21-5p, and miR-21-5p regulated the expression of Tiam1. Down-regulation of circRNA-ACAP2 promoted miR-21-5p expression, which further suppressed the transcription and translation of Tiam1. Conclusion: The present study shows that the circRNA-ACAP2/hsa-miR-21-5p/Tiam1 regulatory feedback circuit could affect the proliferation, migration, and invasion of colon cancer SW480 cells. This was probably due to the fact that circRNA-ACAP2 could act as a miRNA sponge to regulate Tiam1 expression by removing the inhibitory effect of miR-21-5p on Tiam1 expression. The results from this study have revealed new insights into the pathogenicity of colon cancer and may provide novel therapeutic targets for the treatment of colon cancer.

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Section: Discussionmentioning

confidence: 99%

The CircRNA-ACAP2/Hsa-miR-21-5p/ Tiam1 Regulatory Feedback Circuit Affects the Proliferation, Migration, and Invasion of Colon Cancer SW480 Cells

Han

et al. 2018

Cell Physiol Biochem

108

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“…Li et al . found that circHIPK3 exerted inhibitory influence on human invasive bladder cancer T24T and UMUC3 cells by regulating miR‐558 . Additionally, previous researches revealed that circRNA‐MYLK functioned as an endogenous sponge for miR‐29a in bladder cancer.…”

Section: Discussionmentioning

confidence: 95%

Circular RNA CEP128 promotes bladder cancer progression by regulating Mir‐145‐5p/Myd88 via MAPK signaling pathway

Sun

Zhao

Chen

et al. 2019

Intl Journal of Cancer

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The present experiment was designed for exploring the regulatory mechanism of circ‐CEP128/miR‐145‐5p/MYD88 axis in bladder cancer. MiRNAs and circRNAs expression data were derived from Gene Expression Omnibus database with bladder tumor tissues and paracarcinoma tissue samples. Differentially expressed genes in tumor were analyzed via R software. Interaction network of differently expressed miRNAs and differently expressed mRNA was established by means of Cytoscape software. CircCEP128 and miR‐145‐5p expression levels were determined using qRT‐PCR. The expression of MAPK signaling‐related proteins MYD88, p38, ERK and JNK was examined by western blot. The relationship between circCEP128 and miR‐145‐5p was validated using RNA immunoprecipitation. The level of cell propagation and migration was determined by CCK8 and wound healing assay, 5‐bromo‐2′‐deoxyuridine assay and migration assay. Cell apoptosis rate and cell cycle were detected via flow cytometry. Tumor xenograft assay was implemented to investigate the function of circCEP128 in vivo. CircCEP128 and MYD88 were overexpressed in bladder cancer based on microarray analysis and miR‐145‐5p was a potential targeting factor in bladder cancer. CircCEP128 targeted miR‐145‐5p and miR‐145‐5p targeted MYD88. Expression of miR‐145‐5p was decreased in cancer samples. Knockdown of circCEP128 induced the inhibition of cell viability and mobility and cell cycle arrest. Overexpression of miR‐145‐5p or knockdown of circCEP128 promoted MAKP signaling pathway and related proteins expression. In addition, knockdown of circCEP128 suppressed the growth of bladder cancer tumor tissues in vivo. Overexpression of circCEP128 promoted bladder cancer progression through modulating miR‐145‐5p and MYD88 via MAKP signaling pathway.

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“…The pull‐down assay with biotinylated RNA was performed as described . In brief, for circRUNX2 pulled down miRNAs, the biotinylated‐circRUNX2 probe was incubated with C‐1 magnetic beads (Life Technologies, Carlsbad, CA, USA) to generate probe‐coated beads, then incubated with sonicated HBMSCs at 4°C overnight, followed by eluted and qRT‐PCR.…”

Section: Methodsmentioning

confidence: 99%

CircRUNX2 through has‐miR‐203 regulates RUNX2 to prevent osteoporosis

Yin

Wang

et al. 2018

J Cellular Molecular Medi

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ObjectiveWe aimed to discover the molecular mechanism of hsa_circ_0076694 (circRUNX2) on osteogenic differentiation. We also explored the interaction between circRUNX2, miR‐203 and RUNX2.MethodsClinical samples obtained from femoral neck fracture patients’ bone tissues were used to collect circRUNX2, miR‐203, and RUNX2 expression data, while their expression changes were observed in human bone mesenchymal stem cells (hBMSCs) during osteogenic differentiation. QRT‐PCR and Western blot were used to analyse levels of RNAs and proteins. Biotin pull down, RIP, RNA FISH, and Dual‐Luciferase Reporter assays demonstrated the relationship between circRUNX2, miR‐203, and RUNX2. ALP and ARS staining were used to measure the degree of osteogenic differentiation under the control of circRUNX2, miR‐203.ResultsCircRUNX2 were down‐regulated in osteoporotic patients’ bone tissues. CircRUNX2 could inhibit miR‐203 expression by sponging miR‐203. MiR‐203 inhibited osteogenic differentiation by targeting the 3′‐UTR of RUNX2 and down‐regulate RUNX2 expression. Overexpression of circRUNX2 promoted the expression of osteogenic differentiation‐related proteins such as RUNX2, OCN, OPN, BSP, and prevented osteoporosis.ConclusioncircRUNX2 could sponge miR‐203 and enhance RUNX2 expression, thus circRUNX2 prevents osteoporosis and may provide a novel therapeutic strategy for it.

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Circ HIPK 3 sponges miR‐558 to suppress heparanase expression in bladder cancer cells

Cited by 475 publications

References 46 publications

The CircRNA-ACAP2/Hsa-miR-21-5p/ Tiam1 Regulatory Feedback Circuit Affects the Proliferation, Migration, and Invasion of Colon Cancer SW480 Cells

The CircRNA-ACAP2/Hsa-miR-21-5p/ Tiam1 Regulatory Feedback Circuit Affects the Proliferation, Migration, and Invasion of Colon Cancer SW480 Cells

Circular RNA CEP128 promotes bladder cancer progression by regulating Mir‐145‐5p/Myd88 via MAPK signaling pathway

CircRUNX2 through has‐miR‐203 regulates RUNX2 to prevent osteoporosis

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