Circ-RPPH1 knockdown retards breast cancer progression via miR-328-3p-mediated suppression of HMGA2

Li, Jing; Li, Yinmou; Cheng, Hong

doi:10.1016/j.clbc.2021.08.009

Cited by 18 publications

(14 citation statements)

References 35 publications

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“…RPPH1 also enhances cell proliferation in breast cancer and AML cell lines by mechanistically antagonizing specific miRNAs, suggesting RPPH1 may function as a molecular miRNA sponge (Lei et al, 2019;Zhang & Tang, 2017). Consistent with and in addition to these reports, a novel circular form of RPPH1, circ-RPPH1, is elevated in breast cancer, cervical cancer, and hepatocellular carcinoma and in all cases promotes proliferation and tumor growth by sequestering specific miRNAs (Li et al, 2022). The canonical form of RPPH1 ncRNA also interacts with Galectin-3 (Gal-3) and activates related signaling pathways that drive cell proliferation in mesangial cells .…”

Section: Rpph1 and Rmrp: Rna Components Of Ribonuclease P/mrp Family ...supporting

confidence: 69%

The Pol III transcriptome: Basic features, recurrent patterns, and emerging roles in cancer

Zhou

Bortle

2023

WIREs RNA

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The RNA polymerase III (Pol III) transcriptome is universally comprised of short, highly structured noncoding RNA (ncRNA). Through RNA–protein interactions, the Pol III transcriptome actuates functional activities ranging from nuclear gene regulation (7SK), splicing (U6, U6atac), and RNA maturation and stability (RMRP, RPPH1, Y RNA), to cytoplasmic protein targeting (7SL) and translation (tRNA, 5S rRNA). In higher eukaryotes, the Pol III transcriptome has expanded to include additional, recently evolved ncRNA species that effectively broaden the footprint of Pol III transcription to additional cellular activities. Newly evolved ncRNAs function as riboregulators of autophagy (vault), immune signaling cascades (nc886), and translation (Alu, BC200, snaR). Notably, upregulation of Pol III transcription is frequently observed in cancer, and multiple ncRNA species are linked to both cancer progression and poor survival outcomes among cancer patients. In this review, we outline the basic features and functions of the Pol III transcriptome, and the evidence for dysregulation and dysfunction for each ncRNA in cancer. When taken together, recurrent patterns emerge, ranging from shared functional motifs that include molecular scaffolding and protein sequestration, overlapping protein interactions, and immunostimulatory activities, to the biogenesis of analogous small RNA fragments and noncanonical miRNAs, augmenting the function of the Pol III transcriptome and further broadening its role in cancer.This article is categorized under: RNA in Disease and Development > RNA in Disease RNA Processing > Processing of Small RNAs RNA Interactions with Proteins and Other Molecules > Protein‐RNA Interactions: Functional Implications

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Section: Rpph1 and Rmrp: Rna Components Of Ribonuclease P/mrp Family ...supporting

confidence: 69%

The Pol III transcriptome: Basic features, recurrent patterns, and emerging roles in cancer

Zhou

Bortle

2023

WIREs RNA

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“…Circular RNAs are known to impart either tumor suppressive 60 or oncogenic functions in several types of cancers 61 - 63 . However, there are only two comprehensive studies that discuss the cross-talk between regulatory (inhibitory) module of the pathway and circRNAs in cancer.…”

Section: Regulation Of Hippo-yap Signaling Axis By Circrnasmentioning

confidence: 99%

Circular RNAs modulate Hippo-YAP signaling: functional mechanisms in cancer

Qadir¹,

Li²,

Yang³

2022

Theranostics

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The Hippo signaling pathway is an evolutionarily conserved network that regulates organ size and tissue homeostasis in mammals. This pathway controls various cell functions, such as growth, proliferation, survival, apoptosis, and stemness by switching 'on' or 'off' its inhibitory and/or transcriptional module, thereby regulating target gene(s) expression. Altered Hippo signaling has been implicated in various forms of cancers. Increasing evidence suggests cross-talk between the Hippo signaling pathway and non-coding RNAs, in particular circular RNAs (circRNAs). In this context, the current review presents the mechanistic interplay between the Hippo pathway and related circRNAs in various forms of cancers, along with the capabilities of these circRNAs to function either as tumor suppressors or oncogenes through miRNA sponging or protein binding mechanisms. Furthermore, we discuss the constraints and limitations in circRNA mechanistic studies while highlighting some outstanding questions regarding the roles of circRNAs associated with the Hippo-YAP pathway in cancer. Finally, we delineate the potential of these circRNAs to be employed as diagnostic and prognostic biomarkers, as well as molecular hotspots for cancer therapy.

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“…and glycolysis of BC cells. 37 Also, our detection revealed that circSEPT9 was upregulated in BC. Moreover, functional assay indicated that silencing circSEPT9 notably arrested cell proliferation, migration, EMT processes and exhibited a proapoptotic function.…”

Section: Discussionmentioning

confidence: 64%

CircSEPT9 promotes breast cancer progression by regulating PTBP3 expression via sponging miR‐625‐5p

Shi,

Li,

Chen

et al. 2024

Thoracic Cancer

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BackgroundBreast cancer (BC) is a common malignancy which threatens the health of women. Circular RNAs (circRNAs) are critical factors in multiple cancers, including BC. The aim of this experiment was to investigate the molecular mechanisms of circRNA Septin 9 (circSEPT9) in the progression of BC.MethodsCircSEPT9, microRNA‐625‐5p (miR‐625‐5p) and polypyrimidine tract‐binding protein 3 (PTBP3) levels were determined by quantitative real‐time polymerase chain reaction (qRT‐PCR). Western blot was performed to detect the protein levels of PTBP3, E‐cadherin and vimentin. Cell counting kit‐8 assay (CCK8) and thymidine analog 5‐ethynyl‐2′‐deoxyuridine (EDU) was utilized for proliferation examination. Flow cytometry was conducted to measure apoptosis. Transwell assay and wound healing assay to investigate the migration of BC cells. Glucose uptake and lactate production were determined by specific kits. Additionally, dual‐luciferase reporter assay and RNA immunoprecipitation (RIP) assay were utilized to verify the interaction. A murine xenograft model was established to investigate the function of circSEPT9 in BC in vivo.ResultsOverexpression of circSEPT9 was found in BC tissues and cells. Silencing circSEPT9 impeded BC cell proliferation, migration, epithelial–mesenchymal transition (EMT) and glycolytic metabolism but facilitated cell apoptosis in vitro. Meanwhile, circSEPT9 knockdown constrained tumor growth in vivo. MiR‐625‐5p was targeted by circSEPT9. The influence of silencing circSEPT9 on BC cell function was regained by miR‐625‐5p inhibitor. Furthermore, miR‐625‐5p regulated BC cell malignant phenotypes via downregulating PTBP3.ConclusioncircSEPT9 contributed to the malignant progression of BC by up‐regulating PTBP3 via sponging miR‐625‐5p.

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Circ-RPPH1 knockdown retards breast cancer progression via miR-328-3p-mediated suppression of HMGA2

Cited by 18 publications

References 35 publications

The Pol III transcriptome: Basic features, recurrent patterns, and emerging roles in cancer

The Pol III transcriptome: Basic features, recurrent patterns, and emerging roles in cancer

Circular RNAs modulate Hippo-YAP signaling: functional mechanisms in cancer

CircSEPT9 promotes breast cancer progression by regulating PTBP3 expression via sponging miR‐625‐5p

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