Circ-CCS is identified as a cancer-promoting circRNA in lung cancer partly by regulating the miR-383/E2F7 axis

Yuan, Yifang; Zhou, Xiaolei; Kang, Yan; Kuang, Hongping; Peng, Qiang; Zhang, Bo; Liu, Xinxin; Zhang, Manlin

doi:10.1016/j.lfs.2020.118955

Cited by 28 publications

(22 citation statements)

References 32 publications

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“…Because of its ring structure, it can resist the degradation of RNase R, which is relatively stable [ 13 ]. circRNA has attracted more and more attention due to its specificity of expression and complexity of regulation, as well as its important role in disease occurrence [ 14 , 15 ]. Just like miRNA and long noncoding RNA, circRNA has become a new research hotspot in the RNA field [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

[Retracted] Mechanism of RNA circHIPK3 Involved in Resistance of Lung Cancer Cells to Gefitinib

Zhao

Zhang

Tang

et al. 2022

BioMed Research International

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To study the mechanism of circular ribonucleic acid (RNA) circHIPK3 involved in the resistance of lung cancer cells to gefitinib, 110 patients with lung cancer were recruited as the research objects, and the tumor tissue and para-cancerous tissue of each patient’s surgical specimens were collected and paraffinized to detect the expression of circHIPK3 in different tissues. Gefitinib drug-resistant cell line of lung cancer was constructed with gefitinib to detect cell apoptosis under different conditions. As a result, the relative expression of circHIPK3 in patients with tumor diameter no less than 3 cm was dramatically inferior to that in patients with tumor diameter less than 3 cm ( P < 0.05 ). The relative expression of circHIPK3 in patients with TNM stage II/III was dramatically inferior to that in patients with tumor, node, and metastasis (TNM) stage I ( P < 0.05 ). Expression of circHIPK3 in patients with lymph node metastasis was dramatically inferior to that in patients without lymph node metastasis ( P < 0.05 ). Of the lung cancer tissues of patients with different TNM stages, only six patients had high expression, and the remaining 104 patients had low expression. Moreover, electrophoresis revealed that circHIPK3 can only be amplified in cDNA, but not in gDNA. Gefitinib-mediated apoptosis rate of lung cancer drug-resistant cell lines decreased notably. In summary, the circular RNA circHIPK3 may have a notably low expression in lung cancer tissues, whose low expression had a certain enhancement effect on the drug resistance of lung adenocarcinoma cells to gefitinib.

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Section: Introductionmentioning

confidence: 99%

[Retracted] Mechanism of RNA circHIPK3 Involved in Resistance of Lung Cancer Cells to Gefitinib

Zhao

Zhang

Tang

et al. 2022

BioMed Research International

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“…After transfection for 48 h, detection of the luciferase activity of the cells was via dual-luciferase reporter gene assay kit (Promega, Madison, WI, USA). Each experiment was repeated three times [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

“…Pearson correlation analysis was on the association of circ-UBR1 and miR-545-5p, miR-545-5p and SSFA2 in clinical samples. P < 0.05 was accepted as indicative of manifest differences [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

“…For instance, circ_0001073 is reported to repress LC advancement via miR-626/LIFR axis, clarifying the latent value of circ_0001073 in LC therapy [ 11 ]. Meanwhile, a study proposes that circ-CCS is elevated in LC and forecasts unpleasing prognosis [ 12 ]. Currently, molecular targeted therapy clarifies enormous potential in tumor therapy [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Circular RNA UBR1 promotes the proliferation, migration, and invasion but represses apoptosis of lung cancer cells via modulating microRNA-545-5p/SSFA2 axis

Mao

Zhang

et al. 2021

Bioengineered

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Lung cancer (LC) is a malignant tumor with the highest incidence in the world, and its specific pathogenesis is still unclear. Circular RNAs (circRNAs) are a group of non-coding RNAs that play a key role in the development and progression of various cancers. The expression pattern and function of circRNAs in LC are still not completely distinct. In this study, it was aimed to study the expression and potential mechanism of circ-UBR1 in LC cells. Then it was found that circ-UBR1 was up-regulated in LC cells, and had microRNA (miR)-545-5p binding sites. Meanwhile, it was confirmed by dual-luciferase reporter assay that circ-UBR1 directly bound to miR-545-5p and then repressed its expression. MiR-545-5p was down-regulated in LC cells and refrained its expression by binding to the downstream target gene SSFA2. Knockdown circ-UBR1 or enhancive miR-545-5p repressed A549 cell proliferation, migration, and invasion, but accelerated apoptosis. After transfection with circ-UBR1 low expression vector, upregulation of SSFA2 apparently reversed the depression of reduced circ-UBR1 on cell proliferation, migration, and invasion, and the promotion of cell apoptosis. Further tumor xenograft experiments in nude mice also confirmed that knockdown of circ-UBR1 could increase the expression of miR-545-5p, but decrease the expression of SSFA2, thus alleviating the progression of LC in vivo . Therefore, these results fully indicate that circ-UBR1 promotes LC cell proliferation, migration, and invasion, but represses apoptosis via the circ-UBR1 axis, which may be a closely related marker and therapeutic target of LC.

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“…LncRNA-TMPO-AS1 promotes tumor growth, cell migration, and invasion in pancreatic carcinoma by regulating the miR-383/SOX11 axis [35]. Additionally, circRNA-CCS, via sponging miR-383, promotes lung cancer cell growth, metastasis, and predicted poor prognosis [14]. Circ-0136666 was reported to facilitate the carcinogenesis of colorectal cancer via targeting the miR-383/CREB1 axis [106].…”

Section: Regulation Of Mir-383 Expressionmentioning

confidence: 99%

A Concise Review of MicroRNA-383: Exploring the Insights of Its Function in Tumorigenesis

Qian¹,

Xie²,

Sun³

et al. 2022

J. Cancer

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MicroRNAs (miRNAs) are small noncoding RNAs that commonly have 18-22 nucleotides and play important roles in the regulation of gene expression via directly binding to the 3'-UTR of target mRNAs. Approximately 50% of human genes are regulated by miRNAs and they are involved in many human diseases, including various types of cancers. Recently, microRNA-383 (miR-383) has been identified as being aberrantly expressed in multiple cancers, such as malignant melanoma, colorectal cancer, hepatocellular cancer, and glioma. Increasing evidence suggests that miR-383 participates in tumorigenic events including proliferation, apoptosis, invasion, and metastasis as well as drug resistance. Although downstream targets including CCND1, LDHA, VEGF, and IGF are illustrated to be regulated by miR-383, its roles in carcinogenesis are still ambiguous and the underlying mechanisms are still unclear. Herein, we review the latest studies on miR-383 and summarize its functions in human cancers and other diseases. The goal of this review is to provide new strategies for targeted therapy and further investigations.

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Circ-CCS is identified as a cancer-promoting circRNA in lung cancer partly by regulating the miR-383/E2F7 axis

Cited by 28 publications

References 32 publications

[Retracted] Mechanism of RNA circHIPK3 Involved in Resistance of Lung Cancer Cells to Gefitinib

[Retracted] Mechanism of RNA circHIPK3 Involved in Resistance of Lung Cancer Cells to Gefitinib

Circular RNA UBR1 promotes the proliferation, migration, and invasion but represses apoptosis of lung cancer cells via modulating microRNA-545-5p/SSFA2 axis

A Concise Review of MicroRNA-383: Exploring the Insights of Its Function in Tumorigenesis

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