Ciprofloxacin-modified electrosynthesized hydrogel coatings to prevent titanium-implant-associated infections

Giglio, Elvira De; Cometa, Stefania; Ricci, Maria Antonietta; Cafagna, D.; Savino, Antonio; Sabbatini, Luigia; Orciani, Monia; Ceci, Edmondo; Novello, L.; Tantillo, Giuseppina; Mattioli‐Belmonte, Monica

doi:10.1016/j.actbio.2010.07.030

Cited by 90 publications

(67 citation statements)

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“…This macroporous titanium is characterized by multiple layers of hexagonal pores with a 640 lm diameter, 8 macroporosity that facilitates cell migration and bone deposition. 9 It has been shown that porous titanium implants can be enriched, either directly [10][11][12] or by association with biocompatible hydrogels, [13][14][15][16] with antibiotics, 14 VEGF, 15 and FGF-2. 16 To improve implant osseointegration, osteogenic factors can be used for implant enrichment.…”

Section: Introductionmentioning

confidence: 99%

Orthopedic bioactive implants: Hydrogel enrichment of macroporous titanium for the delivery of mesenchymal stem cells and strontium

Lopa¹,

Mercuri

Colombini³

et al. 2013

J Biomedical Materials Res

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Insufficient implant stability is an important determinant in the failure of cementless prostheses. To improve osseointegration, we aim at generating a bioactive implant combining a macroporous titanium (TT) with a biocompatible hydrogel to encapsulate osteo-inductive factors and osteoprogenitor cells. Amidation and cross-linking degree of an amidated carboxymethylcellulose hydrogel (CMCA) were characterized by FT-IR spectrometry and mechanical testing. Bone marrow mesenchymal stem cells (BMSCs) from osteoarthritic patients were cultured on CMCA hydrogels, TT, and TT loaded with CMCA (TT þ CMCA) with an optimized concentration of SrCl 2 to evaluate cell viability and osteo-differentiation. Amidation and cross-linking degree were homogeneous among independent CMCA batches. SrCl 2 at 5 lg/mL significantly improved BMSCs osteo-differentiation increasing calcified matrix (P < 0.01), type I collagen expression (P < 0.05) and alkaline phosphatase activity. TT þ CMCA samples better retained cells into the TT mesh, significantly improving cell seeding efficiency with respect to TT (P < 0.05). BMSCs on TT þ CMCA underwent a more efficient osteo-differentiation with higher alkaline phosphatase (P < 0.05) and calcium levels compared to cells on TT. Based on these in vitro results, we envision the association of TT with strontium-enriched CMCA and BMSCs as a promising strategy to generate bioactive implants promoting bone neoformation at the implant site.

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Section: Introductionmentioning

confidence: 99%

Orthopedic bioactive implants: Hydrogel enrichment of macroporous titanium for the delivery of mesenchymal stem cells and strontium

Lopa¹,

Mercuri

Colombini³

et al. 2013

J Biomedical Materials Res

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“…The PHEMA C 1s fitting gave the functional group ratios similar to those reported in literature ( compared to 2:1:1:1:1). [33] The variation between the experimental and literature ratios could be attributed to the surface not being totally covered by the grafted polymer. PEGgrafted surfaces also exhibited the functionalities expected (-CHx, -COC, and -COH).…”

Section: Characterization Of Surface Chemistriesmentioning

confidence: 97%

“…The experimental spectra were fitted using Gaussian-Lorentzian peaks (85:15 ratio), with each having the same full width at half maximum as detailed by previous fitting models in literature. [33,34] Surface wettability was determined using contact angle analysis by averaging measured contact angles of 20 μL water droplets, distributed randomly across the test surface and utilizing image analysis software ImageJ, Version 1.47.…”

Section: Surface Characterization Techniquesmentioning

confidence: 99%

Plasma engineered surfaces for orthopedic devices

Farhat

Gilliam

Samaniego

et al. 2016

Journal of Biomaterials Science, Polymer Edition

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Atmospheric pressure plasma was used to graft various biocompatible polymers to the surface of ultra-high molecular weight polyethylene (UHMWPE). Polymers used as grafts in this study were poly(2-hydroxyethylmethacrylate) (PHEMA) and polyethylene glycol (PEG). A significant decrease in contact angle was noted for grafted surfaces, indicating increased hydrophilicity. Surface functionalities were verified using Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The wear properties of the coatings were determined by weight loss under conditions of a random motion pin-on-plate apparatus with the coated polyethylene plaques immersed in DI water. Based on these tests, the grafted surfaces exhibited an improved resistance to wear, compared to UHMWPE. Cell viability studies were used to confirm that the plasma treatment had no negative effects on the surface bio-toxicity. Based on the results, it is anticipated that the incorporation of these biocompatible polymer-grafted UHMWPE surfaces in metal-on-plastic orthopedic implants should improve their performance and longevity.

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“…[54][55][56] Hydrogel coatings containing the antibiotic ciprofloxacin are being developed to prevent implant-associated infections in titanium devices. 57 Finally, several coatings that elute drugs, biological factors, and minerals have been investigated with the goal of improving the biological response to growth. PTH-and bisphosphonate-coated stainless steel screws increased pullout strength and energy as well as fixation.…”

Section: Applications Of Biomaterials To Plate and Screw Fixationmentioning

confidence: 99%