Cinnamate 4‐hydroxylase from Catharanthus roseus and a strategy for the functional expression of plant cytochrome P450 proteins as translational fusions with P450 reductase in Escherichia coli

Hotze, Michael; Schröder, G.; Schröder, Joachim

doi:10.1016/0014-5793(95)01141-z

Cited by 107 publications

(8 citation statements)

References 33 publications

(42 reference statements)

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“…The gene expression level of PAL was both elevated obviously in this study, leading to the conversion of phenylalanine into cinnamic acid, which is a precursor for several phenolic compounds such as cinnamic acid, p-coumaric acid, caffeic acid, naringenin, and syringlc acid. The expression of C4H responsible for the hydroxylation at the C-4 position of cinnamic acid to form p -coumaric acid significantly increased, thereby contributing to the C6C3 compounds synthesis (Hotze et al, 1995 ).…”

Section: Discussionmentioning

confidence: 99%

The Combined Effects of Ethylene and MeJA on Metabolic Profiling of Phenolic Compounds in Catharanthus roseus Revealed by Metabolomics Analysis

Liu

Wang

et al. 2016

Front. Physiol.

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Phenolic compounds belong to a class of secondary metabolites and are implicated in a wide range of responsive mechanisms in plants triggered by both biotic and abiotic elicitors. In this study, we approached the combinational effects of ethylene and MeJA (methyl jasmonate) on phenolic compounds profiles and gene expressions in the medicinal plant Catharanthus roseus. In virtue of a widely non-targeted metabolomics method, we identified a total of 34 kinds of phenolic compounds in the leaves, composed by 7 C6C1-, 11 C6C3-, and 16 C6C3C6 compounds. In addition, 7 kinds of intermediates critical for the biosynthesis of phenolic compounds and alkaloids were identified and discussed with phenolic metabolism. The combinational actions of ethylene and MeJA effectively promoted the total phenolic compounds, especially the C6C1 compounds (such as salicylic acid, benzoic acid) and C6C3 ones (such as cinnamic acid, sinapic acid). In contrast, the C6C3C6 compounds displayed a notably inhibitory trend in this case. Subsequently, the gene-to-metabolite networks were drawn up by searching for correlations between the expression profiles of 5 gene tags and the accumulation profiles of 41 metabolite peaks. Generally, we provide an insight into the controlling mode of ethylene-MeJA combination on phenolic metabolism in C. roseus leaves.

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Section: Discussionmentioning

confidence: 99%

The Combined Effects of Ethylene and MeJA on Metabolic Profiling of Phenolic Compounds in Catharanthus roseus Revealed by Metabolomics Analysis

Liu

Wang

et al. 2016

Front. Physiol.

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“…Phenylalanine ammonia lyase (PAL) catalyses the first reaction of the phenylpropanoid pathway by the deamination of phenylalanine into trans -cinnamic acid [27]. A cinnamate 4-hydroxylase (C4H) then catalyses the hydroxylation of trans -cinnamic acid to yield p -coumaric acid [28]. Alternatively, p- coumaric acid can be produced directly from tyrosine by a tyrosine ammonia lyase (TAL), bypassing the C4H intermediate [6].…”

Section: Polyketide Biosynthesis In Plantsmentioning

confidence: 99%

Engineering Microbes for Plant Polyketide Biosynthesis

Lussier

Colatriano

Wiltshire

et al. 2012

Computational and Structural Biotechnology Journal

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Polyketides are an important group of secondary metabolites, many of which have important industrial applications in the food and pharmaceutical industries. Polyketides are synthesized from one of three classes of enzymes differentiated by their biochemical features and product structure: type I, type II or type III polyketide synthases (PKSs). Plant type III PKS enzymes, which will be the main focus of this review, are relatively small homodimeric proteins that catalyze iterative decarboxylative condensations of malonyl units with a CoA-linked starter molecule. This review will describe the plant type III polyketide synthetic pathway, including the synthesis of chalcones, stilbenes and curcuminoids, as well as recent work on the synthesis of these polyketides in heterologous organisms. The limitations and bottlenecks of heterologous expression as well as attempts at creating diversity through the synthesis of novel “unnatural” polyketides using type III PKSs will also be discussed. Although synthetic production of plant polyketides is still in its infancy, their potential as useful bioactive compounds makes them an extremely interesting area of study.

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“…Numerous plant flavonoid biosynthetic enzymes, which are individually expressed in E. coli as functional enzymes, have been identified (26,27). Furthermore, Watts et al .…”

Section: Website Usagementioning

confidence: 99%

FMM: a web server for metabolic pathway reconstruction and comparative analysis

Chou

Chang

Chiu

et al. 2009

Nucleic Acids Research

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Synthetic Biology, a multidisciplinary field, is growing rapidly. Improving the understanding of biological systems through mimicry and producing bio-orthogonal systems with new functions are two complementary pursuits in this field. A web server called FMM (From Metabolite to Metabolite) was developed for this purpose. FMM can reconstruct metabolic pathways form one metabolite to another metabolite among different species, based mainly on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and other integrated biological databases. Novel presentation for connecting different KEGG maps is newly provided. Both local and global graphical views of the metabolic pathways are designed. FMM has many applications in Synthetic Biology and Metabolic Engineering. For example, the reconstruction of metabolic pathways to produce valuable metabolites or secondary metabolites in bacteria or yeast is a promising strategy for drug production. FMM provides a highly effective way to elucidate the genes from which species should be cloned into those microorganisms based on FMM pathway comparative analysis. Consequently, FMM is an effective tool for applications in synthetic biology to produce both drugs and biofuels. This novel and innovative resource is now freely available at http://FMM.mbc.nctu.edu.tw/.

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Cinnamate 4‐hydroxylase from Catharanthus roseus and a strategy for the functional expression of plant cytochrome P₄₅₀ proteins as translational fusions with P₄₅₀ reductase in Escherichia coli

Cited by 107 publications

References 33 publications

The Combined Effects of Ethylene and MeJA on Metabolic Profiling of Phenolic Compounds in Catharanthus roseus Revealed by Metabolomics Analysis

The Combined Effects of Ethylene and MeJA on Metabolic Profiling of Phenolic Compounds in Catharanthus roseus Revealed by Metabolomics Analysis

Engineering Microbes for Plant Polyketide Biosynthesis

FMM: a web server for metabolic pathway reconstruction and comparative analysis

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