Cinnamaldehyde is a biologically active compound for the disinfection of removable denture: blinded randomized crossover clinical study

Abstract

Background: Fungal infections associated with the use of dentures, like denture stomatitis, are difficult to prevent and treat. This in situ study aimed to investigate the efficacy of cinnamaldehyde for the disinfection of complete removable dentures, and the effect on the physical and mechanical properties (Vickers microhardness, color, and surface roughness) of the acrylic resin. Methods: Acrylic resin disks were inserted into the dentures of a probabilistic sample of 33 complete denture users, that used cinnamaldehyde (27 μg/mL) and 0.5% sodium hypochlorite solutions in a 20 min/7-days protocol of dentures immersion in each solution, with a wash-out period of 7 days, to constitute a crossover-study. The disks were analyzed before and after the immersion, for the presence of microorganisms (CFU/mL) and by scanning electron microscope (SEM). Also, the surface roughness (Ra) and Vickers microhardness were measured, and color parameters were analyzed using the National Bureau of Standards (NBS) method. Data was analyzed by Wilcoxon and Friedman (microbiological evaluation), paired t-test (color and roughness) and independent t-test (Vickers hardness) (α = 0.05). Results: A significant reduction (P < 0.05) in the number of microorganisms was observed for each species (total microorganisms, Streptococcus mutans, and Candida spp.), with no significant differences (P > 0.05) between hypochlorite and cinnamaldehyde. There was an increase in the roughness and a decrease in the hardness of the test specimens, with no difference between the two disinfectant substances (P > 0.05). Both hypochlorite and cinnamaldehyde also caused changes in color, considered as "perceptible" by the NBS classification, but with no significant difference between disinfectant substances (P < 0.05), and under the clinically acceptable limit (ΔE ≤ 3.7). Conclusion: The 27 μg/mL cinnamaldehyde solution was effective against all evaluated microorganisms and caused minor alterations in hardness, surface roughness, and color parameters, with no clinical relevance.

Evaluation and Comparison of the Effectiveness of Physical Methods of Disinfection on Heat-Polymerized Polymethyl Methacrylate – An In-vitro Study

“…In removal prosthesis, many disinfectants have been used such as Sodium hypochlorite [7], Glutaraldehyde [8], Chlorine Dioxide [9], and Sodium Perborate Monohydrate [30], etc. The present study was undertaken to quantitatively analyze the candidal growth after acrylic resin specimens contamination and to compare the efficacy of two different commonly used, rapidly effective, cost-effective, non-toxic, easily implemented household disinfection techniques (microwave irradiation and UV radiation) after the contamination procedure.…”

“…The main demerit of the mechanical method, it lacks the capacity to remove a large volume of adherent microorganisms and also elicits scratches on the dental surface [4]. Disinfection using a wide range of chemicals such as vinegar [5], alcohol [6], sodium hypochlorite [7], Glutaraldehyde [8], and chlorine dioxide [9] have been employed in the dental practice. However, the important demerits of these techniques include corrosion, Microwave energy has been proposed in dentistry as a simple alternative to prosthetic disinfection to avoid the problems of chemical disinfection [10].…”