Cinnamaldehyde induces changes in the protein profile of Salmonella Typhimurium biofilm

Silva, Alex Fiori da; Santos, Adriele Rodrigues dos; Trevisan, Daliah Alves Coelho; Ribeiro, Alessandra Braga; Campanerut‐Sá, Paula Aline Zanetti; Kukolj, Caroline; Souza, Emanuel Maltempi de; Cardoso, Rosilene Fressatti; Svidzinski, Terezinha Inez Estivalet; Filho, Benício Alves de Abreu; Machinski, Miguel; Mikcha, Jane Martha Graton

doi:10.1016/j.resmic.2017.09.007

Cited by 28 publications

(18 citation statements)

References 42 publications

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“…Specifically these proteins were upregulated by 7.6fold, 7.6-fold and 7.0-fold, respectively and their expression decreased as a function of cinnamaldehyde concentration, which was administered at a concentration of 624 μg/mL. Thus, these differentially expressed proteins have the potential to be possible targets in new therapeutic strategies for the treatment and prevention of S. Typhimurium biofilm formation [103].…”

Section: Antibiofilm Activity Of Trans-cinnamaldehyde Against Salmonella Typhimuriummentioning

confidence: 99%

“…Silva et al [103] investigated the effect of cinnamaldehyde on biofilms of Salmonella Typhimurium ATCC 14028. Here the group found that the biomass of these biofilms was significantly reduced by 21% after exposure to cinnamaldehyde at 312 μg/mL and by 40% after exposure to cinnamaldehyde at 624 μg/mL.…”

Section: Antibiofilm Activity Of Trans-cinnamaldehyde Against Salmonella Typhimuriummentioning

confidence: 99%

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A review of cinnamaldehyde and its derivatives as antibacterial agents

2019

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There is a continuing rise in the occurrence of multidrug-resistant bacterial infections. Antibiotic resistance to currently available antibiotics has become a global health issue leading to an urgent need for alternative antibacterial strategies. There has been a renewed interest in the development of antibacterial agents from natural sources, and trans-cinnamaldehyde is an example of a naturally occurring compound that has received significant attention in recent years. Trans-Cinnamaldehyde has been shown to possess substantial antimicrobial activity, as well as an array of other medicinal properties, and represents an intriguing hit compound from which a number of derivatives have been developed. In some cases, these derivatives have been shown to possess improved activity, not only compared to trans-cinnamaldehyde but also to commonly used antibiotics. Therefore, understanding the antibacterial mechanisms of action that these compounds elicit is imperative in order to facilitate their development and the development of new antibacterial agents that could exploit similar mechanistic approaches. The purpose of this review is to provide an overview of current knowledge on the antibacterial activity and mechanisms of action of cinnamaldehyde and its derivatives, and to highlight significant contributions made in this research area. It is hoped that the findings presented in this work will aid the future development of new antibacterial agents.

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Section: Antibiofilm Activity Of Trans-cinnamaldehyde Against Salmonella Typhimuriummentioning

confidence: 99%

Section: Antibiofilm Activity Of Trans-cinnamaldehyde Against Salmonella Typhimuriummentioning

confidence: 99%

A review of cinnamaldehyde and its derivatives as antibacterial agents

2019

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“…Previous studies have shown that cinnamaldehyde exhibits a wide range of biological activities including anti-inflammatory, anti-bacterial, and immune-modulating properties ( 1 – 3 ). As the major component of cinnamon, cinnamaldehyde has been traditionally used as a food additive, and registered as a flavoring agent by the Food and Drug Administration and approved for food use ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

Cinnamaldehyde Promotes the Intestinal Barrier Functions and Reshapes Gut Microbiome in Early Weaned Rats

Mao

Lü³

et al. 2021

Front. Nutr.

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Cinnamaldehyde is an aromatic aldehyde isolated from the essential oil of cinnamon. It has been proved to possess various bioactivities such as anti-inflammation, anti-bacteria and antihypertensive. Nevertheless, early weaning could lead to intestinal stress, causing a range of intestinal health problems. The aim of this study is to explore the effects of cinnamaldehyde on gut barrier integrity, inflammatory responses, and intestinal microbiome of early weaned rats. In this study, treatment with cinnamaldehyde (100 or 200 mg/kg bodyweight/day) for 2 weeks significantly promoted the production of mucins in the colonic epithelial tissue of rats. Cinnamaldehyde supplementation significantly upregulated the expression of Muc2, TFF3 and the tight junction proteins (ZO-1, claudin-1, and occludin). Hematoxylin and eosin staining results showed that colonic histopathological changes were recovered by cinnamaldehyde supplementation. The mRNA expression of IL-6 and TNF-α were significantly decreased in the cinnamaldehyde groups while the TNF-α protein levels were significantly decreased in the two cinnamaldehyde groups. Cinnamaldehyde treatment obviously attenuated the activation of NF-κB signaling pathway in rat colonic tissue and suppressed the production of inflammatory cytokines. Furthermore, cinnamaldehyde supplementation remodeled the gut microbiome structure, at the genus level, Akkermansia, Bacteroides, Clostridium III, Psychrobacter, Intestinimonas were increased, whereas those of Ruminococcus, Escherichia/Shigella were obviously decreased in the cinnamaldehyde treated groups. These findings indicated that cinnamaldehyde could effectively enhance intestinal barrier integrity, ameliorate inflammatory responses and remodel gut microbiome in early weaned rats.

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“…The pellet obtained from two assays, as described above, was suspended in lysis buffer with protease inhibitor and subjected to sonication (Silva et al, 2018). The sample was centrifuged at 14000 × g for ten minutes at 4 °C and the supernatant was used for analysis.…”

Section: Protein Extractionmentioning

confidence: 99%

“…Proteomic analysis has been applied to better understand the complexity of biofilm of different bacteria (Giaouris et al, 2013;Mangalappalli-Illathu et al, 2008;Silva et al, 2018) and to evaluate its adaptation to different conditions (Mangalappalli-Illathu et al, 2008;Silva et al, 2018), profiles of resistance and susceptibility to drugs (Magalhães et al, 2017), and tolerance to bioactive agents (Condell et al, 2012). The proteomic approach provides the opportunity to determine the functional genome, but also it facilitates the identification of proteins that may not have been predicted by analysis of the genome (Sharma et al, 2010;Tomlinson and Holt, 2001).…”

Section: Introductionmentioning

confidence: 99%

Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study

Trevisan¹,

Campanerut‐Sá²,

Silva³

et al. 2020

JAB

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Carvacrol presents action in Salmonella Typhimurium biofilms, however the antibiofilm mechanism of this compound has not been fully established yet. In the present study, the aim was to evaluate protein profile changes in S. Typhimurium biofilm treated with carvacrol. Proteomic analysis of treated versus untreated biofilm showed several changes in proteins involved with S. Typhimurium biofilm and antioxidant activity. The proteins DsbA (thiol: disulfide interchange protein DsbA), LuxS (S-ribosylhomocysteine lyase), DksA (RNA polymerase binding transcription factor DksA), and SODs (superoxide dismutases) A, B and C had their synthesis decreased after treatment with carvacrol. These proteins play a key role in S. Typhimurium biofilm formation, demonstrating the dynamic antibiofilm action of carvacrol. The differentially expressed proteins identified provide possible action targets for future studies in order to gain more insight into the mechanism of action of carvacrol on S. Typhimurium biofilm.

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Cinnamaldehyde induces changes in the protein profile of Salmonella Typhimurium biofilm

Cited by 28 publications

References 42 publications

A review of cinnamaldehyde and its derivatives as antibacterial agents

A review of cinnamaldehyde and its derivatives as antibacterial agents

Cinnamaldehyde Promotes the Intestinal Barrier Functions and Reshapes Gut Microbiome in Early Weaned Rats

Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study

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