Cinnamaldehyde Exerts Its Antifungal Activity by Disrupting the Cell Wall Integrity of Geotrichum citri-aurantii

Ouyang, Qi; Duan, Xiaofang; Li, Lü; Tao, Nengguo

doi:10.3389/fmicb.2019.00055

Cited by 129 publications

(64 citation statements)

References 38 publications

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“…This result was similar with the effect of antifungal proteins on cell wall chitin (Gandía et al, 2019). It was reported that the changes of chitin content was related to the activity of related enzymes, such as β-1, 3-glucan synthase and chitinase (OuYang et al, 2019). The present results showed that C 12 O 3 TR stress decreased β-1, 3-glucan synthase activity of P. digitatum but increased the chitinase activity (Figures 6A,B).…”

Section: Discussionsupporting

confidence: 89%

Effects of Peptide C12-OOWW-NH2 on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum

Feng

Wang

et al. 2020

Front. Microbiol.

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In this study, the transcriptional profiling of Penicillium digitatum after C 12 O 3 TR treatment was analyzed by RNA-Seq technology. A total of 2562 and 667 genes in P. digitatum were differentially expressed after 2 and 12 h treatment, respectively. These genes were respectively mapped to 91 and 79 KEGG pathways. The expression patterns of differentially expressed genes (DEGs) at 2 and 12 h were similar, mainly were the metabolic processes in cell wall, cell membrane, genetic information and energy. Particularly, the main metabolic process which was affected by C 12 O 3 TR stress for 2 and 12 h was cell integrity, including cell wall and cell membrane. The changes of chitin in cell wall was observed by Calcofluor White (CFW) staining assay. The weaker blue fluorescence in the cell wall septa, the decrease of β-1, 3-glucan synthase activity and the increase of chitinase and AKP activity showed that C 12 O 3 TR could damage the cell wall integrity. In conclusion, these results suggested that C 12 O 3 TR could inhibit the growth of P. digitatum through various mechanisms at transcriptional level, and could influence the cell wall permeability and integrity.

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Section: Discussionsupporting

confidence: 89%

Effects of Peptide C12-OOWW-NH2 on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum

Feng

Wang

et al. 2020

Front. Microbiol.

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“…Both CHI and β-Glu are considered to be two key cell wall-degrading enzymes that openly inhibit the growth of pathogens primarily by the decomposition of chitin and glucan in the cell wall of many pathogenic fungi [51]. In our study, both the chitin and glucan contents of P. italicum mycelia were significantly inhibited by P7G at EC 50 and MIC ( Figure 6E,F), suggesting that P7G treatment could significantly suppress the biosynthesis of chitin and glucan, which is highly consistent with a recent report by Ouyang et al [52]. Moreover, P7G treatment significantly increased the activities of the two cell wall-degrading enzymes CHI and β-Glu in P. italicum mycelia compared to that observed in the control mycelia ( Figure 7A,B).…”

Section: Discussionsupporting

confidence: 92%

Pinocembrin-7-Glucoside (P7G) Reduced Postharvest Blue Mold of Navel Orange by Suppressing Penicillium italicum Growth

Chen

Wan

2020

Microorganisms

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The current study aimed to examine the in vitro and in vivo antifungal potential of pinocembrin-7-glucoside (P7G). P7G is an antifungal flavanone glycoside isolated from Ficus hirta Vahl. fruit against Penicillium italicum, a causative pathogen of blue mold disease in citrus fruit, and this study elucidates its possible action mechanism. P7G had a prominent mycelial growth inhibitory activity against P. italicum, with an observed half maximal effective concentration, minimum inhibitory concentration and minimum fungicidal concentration of 0.08, 0.2, and 0.8 g/L, respectively. The data from the in vivo test show that P7G significantly reduced blue mold symptoms and disease development of P. italicum in artificially inoculated “Newhall” navel orange. Compared to the control, increases in the cell membrane permeability of P. italicum supernatant and decreases in the intracellular constituent (e.g., soluble protein, reducing sugar, and total lipid) contents of P. italicum mycelia were identified, supporting scanning electron microscopy and transmission electron microscopy observations. Furthermore, a marked decline in both chitin and glucanase contents of P. italicum mycelia treated with P7G was induced by increasing its related degrading enzyme activities, suggesting that the cell wall structure was destroyed. The current study indicated that P7G may be a novel alternative for reducing blue mold by suppressing mycelial growth of P. italicum via a cell membrane/wall-targeting mechanism.

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“…Some EOs, such as the ones from A. sativum , C. capitatus , C. cassia , C. zeylanicum , C. citratus , C. flexuosus , E. caryophyllus , and L. citrata , have a generalist effect, and are active on several pathogens (between 7 and 10). These oils are rich in phenols, phenylpropanoids, organosulfur compounds, and/or aldehydes, known in the literature to have antifungal effects (thymol and carvacrol for C. capitatus [ 22 ]; neral and geranial for C. citratus , C. flexuosus , and L. citrata [ 23 ]; eugenol for E. caryophyllus and C. zeylanicum [ 24 ]; cinnamaldehyde for C. cassia and C. zeylanicum [ 25 ]; and diallyl di and tri-sulfide for A. sativum [ 26 ]).…”

Section: Discussionmentioning

confidence: 99%

Screening of Antifungal and Antibacterial Activity of 90 Commercial Essential Oils against 10 Pathogens of Agronomical Importance

Clerck

Maso

Parisi

et al. 2020

Foods

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Nowadays, the demand for a reduction of chemical pesticides use is growing. In parallel, the development of alternative methods to protect crops from pathogens and pests is also increasing. Essential oil (EO) properties against plant pathogens are well known, and they are recognized as having an interesting potential as alternative plant protection products. In this study, 90 commercially available essential oils have been screened in vitro for antifungal and antibacterial activity against 10 plant pathogens of agronomical importance. EOs have been tested at 500 and 1000 ppm, and measures have been made at three time points for fungi (24, 72 and 120 h of contact) and every two hours for 12 h for bacteria, using Elisa microplates. Among the EOs tested, the ones from Allium sativum, Corydothymus capitatus, Cinnamomum cassia, Cinnamomum zeylanicum, Cymbopogon citratus, Cymbopogon flexuosus, Eugenia caryophyllus, and Litsea citrata were particularly efficient and showed activity on a large panel of pathogens. Among the pathogens tested, Botrytis cinerea, Fusarium culmorum, and Fusarium graminearum were the most sensitive, while Colletotrichum lindemuthianum and Phytophthora infestans were the less sensitive. Some EOs, such as the ones from A. sativum, C. capitatus, C. cassia, C. zeylanicum, C. citratus, C. flexuosus, E. caryophyllus, and L. citrata, have a generalist effect, and are active on several pathogens (7 to 10). These oils are rich in phenols, phenylpropanoids, organosulfur compounds, and/or aldehydes. Others, such as EOs from Citrus sinensis, Melaleucacajputii, and Vanilla fragrans, seem more specific, and are only active on one to three pathogens. These oils are rich in terpenes and aldehydes.

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Cinnamaldehyde Exerts Its Antifungal Activity by Disrupting the Cell Wall Integrity of Geotrichum citri-aurantii

Cited by 129 publications

References 38 publications

Effects of Peptide C12-OOWW-NH2 on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum

Effects of Peptide C12-OOWW-NH2 on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum

Pinocembrin-7-Glucoside (P7G) Reduced Postharvest Blue Mold of Navel Orange by Suppressing Penicillium italicum Growth

Screening of Antifungal and Antibacterial Activity of 90 Commercial Essential Oils against 10 Pathogens of Agronomical Importance

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