Cinnamaldehyde and cinnamaldehyde derivatives reduce virulence in Vibrio spp. by decreasing the DNA-binding activity of the quorum sensing response regulator LuxR

Brackman, Gilles; Defoirdt, Tom; Miyamoto, Carol M.; Bossier, Peter; Calenbergh, Serge Van; Nelis, Hans; Coenye, Tom

doi:10.1186/1471-2180-8-149

Cited by 266 publications

(190 citation statements)

References 47 publications

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“…Our experiments are consistent with the notion that NC can interact with E. coli driven by an electrostatic interaction. The main significance of these results lies in the following: 1) Chitosancoated NC are non-toxic, even when they promote the aggregation of E. coli when added at a very narrow range of concentration; 2) NC themselves can inhibit the "hearing" or "sensing" component of bacterial QS; 3) In the same manner that 3OC6HSL was loaded into the oil core of NC, and it was delivered effectively to bacteria to trigger their QS response, it should be feasible to load compounds with QS inhibitory activity, such as cinnamaldehyde [62,63] and other. …”

Section: Resultsmentioning

confidence: 99%

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

Qin

Engwer

Desai

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Highlights Chitosan-coated nanocapsules, but not nanoemulsions, influence the -potential of E.coli  A fixed number of nanocapsules binds and promotes aggregation of bacteria  In silico simulations agree closely with experimental results  Chitosan-coated nanocapsules attenuate the bacterial quorum sensing response § These authors contributed equally to this publication * Author for correspondence Colloids and Surfaces B: Biointerfaces 149 (2017) 358-368 AbstractWe examined the interaction between chitosan-based nanocapsules (NC), with average hydrodynamic diameter ~114-155 nm, polydispersity ~0.127, and -potential ~+50 mV, and an E. coli bacterial quorum sensing reporter strain. Dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) allowed full characterization and assessment of the absolute concentration of NC per unit volume in suspension. By centrifugation, DLS, and NTA, we determined experimentally a "stoichiometric" ratio of ~80 NC/bacterium. By SEM it was possible to image the aggregation between NC and bacteria. Moreover, we developed a custom in silico platform to simulate the behavior of particles with diameters of 150 nm and -potential of +50 mV on the bacterial surface. We computed the detailed force interactions between NC-NC and NC-bacteria and found that a maximum number of 145 particles might interact at the bacterial surface. Additionally, we found that the "stoichiometric" ratio of NC and bacteria has a strong influence on the bacterial behavior and influences the quorum sensing response, particularly due to the aggregation driven by NC.

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Section: Resultsmentioning

confidence: 99%

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

Qin

Engwer

Desai

et al. 2017

Colloids and Surfaces B: Biointerfaces

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“…Studies have demonstrated its ability to disrupt QS signaling in Vibrio (281,282) and Burkholderia (283) species, and it or its structural analogues were effective at protecting Artemia shrimp (281) and C. elegans (284) from V. harveyi, V. anguilarum, and V. vulnificus infections while not inhibiting growth of the bacteria. The proposed mechanism of action for cinnamaldehyde is disruption of protein-DNA interactions of the QS-responsive master regulatory protein, LuxR (not to be confused with LuxR-type AHL receptors), with targeted promoter sequences.…”

Section: Natural-product Qs Inhibitorsmentioning

confidence: 99%

“…The proposed mechanism of action for cinnamaldehyde is disruption of protein-DNA interactions of the QS-responsive master regulatory protein, LuxR (not to be confused with LuxR-type AHL receptors), with targeted promoter sequences. SAR studies found that cinnamaldehyde analogues disrupted, modestly, LuxR's interaction with the LuxR consensus binding sequence (281); however, mechanisms explaining these effects have not been clear, and the knowledge that cinnamaldehyde is able to covalently modify cysteine residues of various proteins (285) leaves open the possibility that other targets are at play. The decreased toxicity of food and herbal extracts compared with other better-studied but cytotoxic QS inhibitors offers a practical rationale to mine natural dietary substances for novel QSI compounds.…”

Section: Natural-product Qs Inhibitorsmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

658

524

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SUMMARY Cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. Its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. With the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. An antivirulence approach by which quorum sensing is impeded has caught on as a viable means to manipulate bacterial processes, especially pathogenic traits that are harmful to human and animal health and agricultural productivity. The identification and development of chemical compounds and enzymes that facilitate quorum-sensing inhibition (QSI) by targeting signaling molecules, signal biogenesis, or signal detection are reviewed here. Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed. Nevertheless, various QSI successes have rightfully perpetuated excitement surrounding new potential therapies, and this review highlights promising QSI leads in disrupting pathogenesis in both plants and animals.

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“…Vibrio vulnificus showed enhanced sensitivity towards doxycycline, in a report by Brackman et al (2008).…”

Section: Synergistic Effect Of Cyanidinmentioning

confidence: 94%

Cyanidin inhibits quorum signalling pathway of a food borne opportunistic pathogen

Gopu

Shetty

2016

J Food Sci Technol

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Quorum sensing (QS) is the process of population dependent cell to cell communication used by bacteria to regulate their phenotypic characteristics. Key virulence factors that determine the bacterial pathogenicity and food spoilage were found to be regulated by QS mechanism. Hence, disrupting the QS signaling pathway could be an attractive strategy to manage food borne pathogens. In the current study, QS inhibitory activity of a naturally occurring anthocyanincyanidin and its anti-biofilm property were assessed against an opportunistic pathogen Klebsiella pneumoniae, using a biosensor strain. Further, QS inhibitory property of a naturally occurring anthocyanin cyanidin was further confirmed using in-silico techniques like molecular docking and molecular dynamics simulation studies. Cyanidin at sub-lethal dose significantly inhibited QS-dependent phenotypes like violacein production (73.96 %), biofilm formation (72.43 %), and exopolysaccharide production (68.65) in a concentrationdependent manner. Cyanidin enhanced the sensitivity of test pathogen to conventional antibiotics in a synergistic manner. Molecular docking analysis revealed that cyanidin binds more rigidly with LasR receptor protein than the signaling compound with a docking score of −9.13 Kcal/mol. Molecular dynamics simulation predicted that QS inhibitory activity occurs through the conformational changes between the receptor and cyanidin complex. Our results indicate that cyanidin, can be a potential QS based antibiofilm and antibacterial agent for food borne pathogens.

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Cinnamaldehyde and cinnamaldehyde derivatives reduce virulence in Vibrio spp. by decreasing the DNA-binding activity of the quorum sensing response regulator LuxR

Cited by 266 publications

References 47 publications

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

Cyanidin inhibits quorum signalling pathway of a food borne opportunistic pathogen

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