Cineromycin B isolated from Streptomyces cinerochromogenes inhibits adipocyte differentiation of 3T3-L1 cells via Krüppel-like factors 2 and 3

Matsuo, Hirotaka; Kondo, Yoshiyuki; Kawasaki, Takashi; Imamura, Nobutaka

doi:10.1016/j.lfs.2015.05.020

Cited by 10 publications

(9 citation statements)

References 28 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Particularly, compounds such as quercetin [ 33 , 34 ] and resveratrol [ 35 , 36 ] inhibit adipogenesis in 3T3-L1 adipocytes. Moreover, these cells have been used to describe the effect of melatonin [ 37 ], reactive oxygen species (ROS), or antioxidants [ 38 ] on adipogenic differentiation [ 39 ], and also, the role of some androgens such as testosterone that inhibit the adipogenic differentiation by activation of androgen receptor/β-catenin/T-cell factor 4 interaction in 3T3-L1 adipocytes [ 40 ]. Apart from that, 3T3-L1 cells have been useful to study the mechanisms of action during the differentiation process of several compounds or nutrients that have previously been shown in vivo to inhibit obesity [ 41 ].…”

Section: Animal Cell Modelsmentioning

confidence: 99%

Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review

Ruiz‐Ojeda

Rupérez

Gómez-Llorente

et al. 2016

IJMS

293

221

View full text Add to dashboard Cite

Over the last several years, the increasing prevalence of obesity has favored an intense study of adipose tissue biology and the precise mechanisms involved in adipocyte differentiation and adipogenesis. Adipocyte commitment and differentiation are complex processes, which can be investigated thanks to the development of diverse in vitro cell models and molecular biology techniques that allow for a better understanding of adipogenesis and adipocyte dysfunction associated with obesity. The aim of the present work was to update the different animal and human cell culture models available for studying the in vitro adipogenic differentiation process related to obesity and its co-morbidities. The main characteristics, new protocols, and applications of the cell models used to study the adipogenesis in the last five years have been extensively revised. Moreover, we depict co-cultures and three-dimensional cultures, given their utility to understand the connections between adipocytes and their surrounding cells in adipose tissue.

show abstract

Section: Animal Cell Modelsmentioning

confidence: 99%

Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review

Ruiz‐Ojeda

Rupérez

Gómez-Llorente

et al. 2016

IJMS

293

221

View full text Add to dashboard Cite

show abstract

“…It was found that compounds such as quercetin and resveratrol [67] inhibit adipogenesis in 3T3-L1 adipocytes. Additionally, these cells have been used to describe the effect of reactive oxygen species, antioxidants or melatonin on adipogenic differentiation [68]. Furthermore, different gene silencing techniques have been applied to study the function of various genes associated with adipogenesis in 3T3-L1 cells, particularly inflammatory pathways, adipokine synthesis and enzyme's function [69].…”

Section: Animal Cell Modelsmentioning

confidence: 99%

Drug Treatment of Obesity: From Bench to Bedside

Jakab¹,

Stoehr²,

Včev³

et al. 2018

Drug Discovery - Concepts to Market

View full text Add to dashboard Cite

Obesity is a complex metabolic and behavioural disorder associated with increased health risk, including coronary artery disease, congestive heart failure and sudden cardiac death. Effective prevention and treatment strategies for obesity are needed. This unmet need for efficient and safe antiobesity medication resulted in many new therapies at various stages of development. Obesity has become one of the most intensively studied diseases because of the availability of suitable animal and cell culture models of adipocyte differentiation and appetite regulation.

show abstract

“…7-O-demethyl albocycline (cineromycin B), a 14-membered ring macrolide antibiotic, was reported bioactive against gram-positive bacteria by the inhibition of their protein synthesis by binding to the 50 s subunit of 70 s ribosomes (Elleuch et al 2010). It also reported as an anti-adipogenesis agent (Matsuo et al 2015). The compound albocycline was previously reported for antibacterial (Harada et al 1984) and anti-mold properties (Zucchi et al 2014), while 7-o-demethoxy-7-oxo albocycline (dehydrocineromycin B) acts only on bacteria (Burkhardt et al 1996).…”

Section: Esi-lc-ms/ms Analysismentioning

confidence: 99%

Isolation, identification, optimization, and metabolite profiling of Streptomyces sparsus VSM-30

et al. 2017

View full text Add to dashboard Cite

Deep sea sediment samples of Bay of Bengal (Visakhapatnam) have been analyzed for actinomycetes as an elite source to screen for the production of bioactive metabolites. The actinomycetes strain VSM-30 has an exciting bioactivity profile and was isolated during our systemic screening of marine actinomycetes. It was identified as Streptomyces sparsus based on morphological, physiological, biochemical, and molecular approaches. Response surface methodology regression analysis was carried out to fit the experimental data of each response by the second-order polynomial. The results have proven right interaction among process variables at optimized values of incubation time at 12 days, pH at 8, temperature at 30 °C, concentrations of starch at 1%, and tryptone at 1% and the data have been adequately fitted into the second-order polynomial models. Under these conditions, the responses (zones of inhibition) of plant pathogenic fungi Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Fusarium solani, and Penicillium citrinum were also matched with experimental and predicted results. Chemotypic analysis of ethyl acetate extract of the strain was done using LC-Q-TOF-MS revealed the presence of bioactive compounds including tryptophan dehydrobutyrine diketopiperazine, maculosin, 7-o-demethyl albocycline, albocycline M-2, and 7-o-demethoxy-7-oxo albocycline in a negative ion mode. The ethyl acetate extract of actinobacterium has been subjected to gas chromatography and mass spectroscopy (GC-MS) revealed the presence of diverse compounds such as dotriacontane, tetracosane 11-decyl-, diheptyl phthalate, 1-hexadecanesulfonyl chloride, L-alanyl-L-tryptophan, phthalic acid ethyl pentyl ester, 4-trifluoroacetoxyhexadecane, and 1H-imidazole 4,5-dihydro-2,4-dimethyl. Hence, the ethyl acetate extract of Streptomyces sparsus VSM-30 may have antibacterial, antifungal, and antioxidant activities due to the presence of secondary metabolites in ethyl acetate extract. The study also supports marine sediment samples of Bay of Bengal, a promising marine ecosystem remained to be explored for new bioactive compounds.

show abstract

Cineromycin B isolated from Streptomyces cinerochromogenes inhibits adipocyte differentiation of 3T3-L1 cells via Krüppel-like factors 2 and 3

Cited by 10 publications

References 28 publications

Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review

Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review

Drug Treatment of Obesity: From Bench to Bedside

Isolation, identification, optimization, and metabolite profiling of Streptomyces sparsus VSM-30

Contact Info

Product

Resources

About