Cinderella no longer: α-catenin steps out of cadherin's shadow

Scott, Jeanie A.; Yap, Alpha S.

doi:10.1242/jcs.03267

Cited by 55 publications

(51 citation statements)

References 62 publications

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“…Both a-catenin and, recently, CAR have been shown to interact with the cytoskeleton (Scott and Yap, 2006;Fok et al, 2007). In agreement with these studies, we noted substantial changes in cell morphology, particularly when using three-dimensional culturing, of cell lines after CAR knockdown.…”

Section: Discussionsupporting

confidence: 90%

“…Our data show that loss of CAR impairs the expression of a-catenin, a protein that has been extensively studied for its inhibition of cancer cell growth and motility (Scott and Yap, 2006). Moreover, loss of a-catenin is frequently described in carcinomas, for example, colon cancer, significantly correlating with depth of invasion, presence of metastasis, and an adverse clinical outcome (Ropponen et al, 1999).…”

Section: Discussionmentioning

confidence: 75%

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Loss of Coxsackie and adenovirus receptor downregulates α-catenin expression

et al. 2009

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BACKGROUND: The Coxsackie and adenovirus receptor (CAR) has been shown to inhibit cancer cell proliferation, migration, and invasion. The underlying mechanisms, however, are poorly understood. METHODS: The differential gene expression in the human colon cancer cell line DLD1 on RNAi-mediated functional CAR knockdown was analysed using oligo-array technology. Expression of a-catenin was determined by quantitative RT-PCR and western blotting. Proliferation, migration, and invasion after CAR knockdown were assessed by in vitro assays, and cell morphology in a threedimensional context was evaluated using matrigel. RESULTS: Oligo-array technology identified a-catenin as the strongest downregulated gene after CAR knockdown. Western blotting and quantitative RT-PCR confirmed a reduced a-catenin expression after CAR knockdown in DLD1 cells and in the rat intestinal cell line IEC-6. Functionally, both cell lines showed a marked increase in proliferation, migration, and invasion on CAR knockdown. In matrigel, both cell lines formed amorphous cell clusters in contrast to well-organised three-dimensional structures of CAR-expressing vector controls. Ectopic 're'-expression of a-catenin in DLD1 and IEC-6 CAR knockdown cells reversed these functional and morphological effects. CONCLUSION: These data suggest that an interaction of CAR and a-catenin mediates the impact of CAR on cell proliferation, migration, invasion, and morphology.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 75%

Loss of Coxsackie and adenovirus receptor downregulates α-catenin expression

et al. 2009

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“…Classic catenins. Catenins comprise a family of well studied proteins that support adherens junction function in different epithelia Scott and Yap, 2006) including the testis (Chen et al, 2003b;Lee et al, 2003;. Catenins have long been regarded as proteins that link cadherins to the actin cytoskeleton either directly or indirectly ( Fig.…”

Section: A Cell-cell Actin-based Adherens Junctionsmentioning

confidence: 99%

Anchoring Junctions As Drug Targets: Role in Contraceptive Development

2008

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“…This finding is in line with other recent studies addressing the function of α-catenin, which is regarded now as a signaling molecule and a regulator of actin dynamics, rather than just a physical link connecting cadherin complex with the actin cytoskeleton. [13][14][15][16][17][18]31 How could α-catenin membrane targeting affect stability of noncentrosomal MTs? α-Catenin was shown to function as an element of several signaling cascades in different cellular systems.…”

Section: Discussionmentioning

confidence: 99%

Signaling function of α-catenin in microtubule regulation

Shtutman

Chausovsky²,

Prager‐Khoutorsky³

et al. 2008

Cell Cycle

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Centrosomes control microtubule dynamics in many cell types, and their removal from the cytoplasm leads to a shift from dynamic instability to treadmilling behavior and to a dramatic decrease of microtubule mass (Rodionov et al., 1999; PNAS 96:115). In cadherin-expressing cells, these effects can be reversed: non-centrosomal cytoplasts that form cadherin-mediated adherens junctions display dense arrays of microtubules (Chausovsky et al., 2000; Nature Cell Biol 2:797). In adherens junctions, cadherin's cytoplasmic domain binds p120 catenin and β-catenin, which in turn binds α-catenin. To elucidate the roles of the cadherin-associated proteins in regulating microtubule dynamics, we prepared GFP-tagged, plasma membrane targeted or untargeted p120 catenin, α-catenin and β-catenin and tested their ability to rescue the loss of microtubule mass caused by centrosomal removal in the poorly adhesive cell line CHO-K1. Only membrane targeting of α-catenin led to a significant increase in microtubule length and density in centrosome-free cytoplasts. Expression of nonmembrane-targeted α-catenin produced only a slight effect, while both membrane-targeted and non-targeted p120 and β-catenin were ineffective in this assay. Together, these findings suggest that α-catenin is able to regulate microtubule dynamics in a centrosome-independent manner.

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Cinderella no longer: α-catenin steps out of cadherin's shadow

Abstract: SummaryCinderella no longer: a a a a-catenin steps out of cadherin's shadow

Cited by 55 publications

References 62 publications

Loss of Coxsackie and adenovirus receptor downregulates α-catenin expression

Loss of Coxsackie and adenovirus receptor downregulates α-catenin expression

Anchoring Junctions As Drug Targets: Role in Contraceptive Development

Signaling function of α-catenin in microtubule regulation

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Cinderella no longer: α-catenin steps out of cadherin's shadow

Abstract: SummaryCinderella no longer: a a a a-catenin steps out of cadherin's shadow

Cited by 55 publications

References 62 publications

Loss of Coxsackie and adenovirus receptor downregulates α-catenin expression

Loss of Coxsackie and adenovirus receptor downregulates α-catenin expression

Anchoring Junctions As Drug Targets: Role in Contraceptive Development

Signaling function of &alpha;-catenin in microtubule regulation

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Signaling function of α-catenin in microtubule regulation