Cilostazol pretreatment prevents PTSD-related anxiety behavior through reduction of hippocampal neuroinflammation

Sadeghi, Maryam; Hemmati, Sara; Yousefi‐Manesh, Hasan; Foroutani, Laleh; Nassireslami, Ehsan; Zoshk, Mojtaba Yousefi; Hosseini, Yasaman; Abbasian, Kourosh; Dehpour, Ahmad Reza; Chamanara, Mohsen

doi:10.1007/s00210-023-02578-3

Cited by 2 publications

(2 citation statements)

References 78 publications

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“…OFT is often used to assess anxiety in laboratory animals ( Sadeghi et al, 2024 ); anxiety in the tested animals is reflected by their total entries into the field and the amount of time they spend in the center of the arena ( Zhao et al, 2022 ), where higher degrees of anxiety are associated with less open-field exploration and more cautious behaviors ( Zhang et al, 2021 ). The number of entries into the center and percentage of total movement spent in the center for the rats in four groups differed statistically significantly [(F (3, 76) = 3.481, p = 0.02) and (F (3, 76) = 4.249, p = 0.008), respectively].…”

Section: Resultsmentioning

confidence: 99%

“…PTSD is associated with anxiety and deficits in social interaction, cognition, and memory ( Wilkins et al, 2021 ). Among them, anxiety is a common symptom in patients with PTSD ( Sadeghi et al, 2024 ). PTSD-related anxiety symptoms stem from the generalization of individual’s initial fear memory, which can trigger anxiety reactions even when exposed to backgrounds similar to the original trauma background ( Dunsmoor and Paz, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Artemisinin reduces PTSD-like symptoms, improves synaptic plasticity, and inhibits apoptosis in rats subjected to single prolonged stress

Liu,

Ding,

Wang

et al. 2024

Front. Pharmacol.

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Post-Traumatic Stress Disorder (PTSD) is a chronic mental disorder characterized by symptoms of panic and anxiety, depression, impaired cognitive functioning, and difficulty in social interactions. While the effect of the traditional Chinese medicine artemisinin (AR) on PTSD is unknown, its therapeutic benefits have been demonstrated by studies on models of multiple neurological disorders. This study aimed to extend such findings by investigating the effects of AR administration on a rat model of PTSD induced by a regimen of single prolonged stress (SPS). After rats were subjected to the SPS protocol, AR was administered and its impact on PTSD-like behaviors was evaluated. In the present study, rats were subjected to a multitude of behavioral tests to evaluate behaviors related to anxiety, memory function, and social interactions. The expression of hippocampal synaptic plasticity-related proteins was detected using Western blot and immunofluorescence. The ultrastructure of synapses was observed under transmission electron microscopy. The apoptosis of hippocampal neurons was examined with Western blot, TUNEL staining, and HE staining. The results showed that AR administration alleviated the PTSD-like phenotypes in SPS rats, including behavior indicative of anxiety, cognitive deficits, and diminished sociability. AR administration was further observed to improve synaptic plasticity and inhibit neuronal apoptosis in SPS rats. These findings suggest that administering AR after the onset of severe traumatic events may alleviate anxiety, cognitive deficits, and impaired social interaction, improve synaptic plasticity, and diminish neuronal apoptosis. Hence, the present study provides evidence for AR’s potential as a multi-target agent in the treatment of PTSD.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Artemisinin reduces PTSD-like symptoms, improves synaptic plasticity, and inhibits apoptosis in rats subjected to single prolonged stress

Liu,

Ding,

Wang

et al. 2024

Front. Pharmacol.

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Putative Pharmacological Depression and Anxiety-Related Targets of Calcitriol Explored by Network Pharmacology and Molecular Docking

Kouba,

Altê,

Rodrigues

2024

Pharmaceuticals

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Depression and anxiety disorders, prevalent neuropsychiatric conditions that frequently coexist, limit psychosocial functioning and, consequently, the individual’s quality of life. Since the pharmacological treatment of these disorders has several limitations, the search for effective and secure antidepressant and anxiolytic compounds is welcome. Vitamin D has been shown to exhibit neuroprotective, antidepressant, and anxiolytic properties. Therefore, this study aimed to explore new molecular targets of calcitriol, the active form of vitamin D, through integrated bioinformatic analysis. Calcitriol targets were predicted in SwissTargetPrediction server (2019 version). The disease targets were collected by the GeneCards database searching the keywords “depression” and “anxiety”. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to analyze the intersections of targets. Network analyses were carried out using GeneMania server (2023 version) and Cytoscape (V. 3.9.1.) software. Molecular docking predicted the main targets of the network and Ligplot predicted the main intermolecular interactions. Our study showed that calcitriol may interact with multiple targets. The main targets found are the vitamin D receptor (VDR), histamine H3 receptor (H3R), endocannabinoid receptors 1 and 2 (CB1 and CB2), nuclear receptor NR1H3, patched-1 (PTCH1) protein, opioid receptor NOP, and phosphodiesterase enzymes PDE3A and PDE5A. Considering the role of these targets in the pathophysiology of depression and anxiety, our findings suggest novel putative mechanisms of action of vitamin D as well as new promising molecular targets whose role in these disorders deserves further investigation.

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Cilostazol pretreatment prevents PTSD-related anxiety behavior through reduction of hippocampal neuroinflammation

Cited by 2 publications

References 78 publications

Artemisinin reduces PTSD-like symptoms, improves synaptic plasticity, and inhibits apoptosis in rats subjected to single prolonged stress

Artemisinin reduces PTSD-like symptoms, improves synaptic plasticity, and inhibits apoptosis in rats subjected to single prolonged stress

Putative Pharmacological Depression and Anxiety-Related Targets of Calcitriol Explored by Network Pharmacology and Molecular Docking

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