Cilostamide and forskolin treatment during pre-IVM improves preimplantation development of cloned embryos by influencing meiotic progression and gap junction communication in pigs

Park, Bola; Lee, Hanna; Lee, Yongjin; Elahi, Fazle; Lee, Joohyeong; Lee, Seung Tae; Park, Choon‐Keun; Hyun, Sang‐Hwan; Lee, Eunsong

doi:10.1016/j.theriogenology.2016.02.029

Cited by 24 publications

(19 citation statements)

References 47 publications

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“…A previous mouse and bovine study using cumulus-oocyte complex (COC) [ 12 ] demonstrated that IBMX treatment for 1–2 h pre-IVM increased COC cAMP levels 100-fold and improved embryo cleavage, blastocyst rates, and embryo quality. Additionally, the pre-IVM treatment had a positive influence on the developmental competence of oocytes in pigs by improving cytoplasmic maturation [ 13 ]. In the present study, we also added low concentration of FSH during pre-IVM treatment, expecting to increase cAMP levels in oocytes.…”

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confidence: 99%

Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes

Abdel-Ghani

Sakaguchi

Kanno

et al. 2018

Journal of Reproduction and Development

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We investigated the effects of pre-maturational (pre-IVM) culture on the developmental competence of small-sized bovine oocytes (110 and < 115 µm). Oocytes were cultured with 3-isobutyl-1-methylxanthine (IBMX) for 0, 5, or 10 h and subjected to in vitro maturation, fertilization, and culture. The cleavage rate (73%) of small-sized oocytes with 5 h pre-IVM was higher than those with 0 and 10 h pre-IVM (61 and 62%, respectively). The blastocyst rate (16%) of embryos derived from small-sized oocytes with 5 h pre-IVM was higher than those with 0 and 10 h pre-IVM (9 and 8%, respectively). In addition, small-sized oocytes with 5 h pre-IVM had a higher mean cell number in blastocysts (134.1 ± 34.8) than those with 0 and 10 h pre-IVM (100.2 ± 17.2 and 107.8 ± 23.7, respectively). In conclusion, the pre-IVM of small-sized oocytes with IBMX for 5 h improved the developmental competence of bovine oocytes, as well as the quality of blastocysts.

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confidence: 99%

Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes

Abdel-Ghani

Sakaguchi

Kanno

et al. 2018

Journal of Reproduction and Development

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“…However, in most of the above cited cases the reported results showed only a slight improvement in developmental competence depending on the approach used. Often, rather than increasing the number of blastocysts obtained, an improvement in the parameters related to embryo quality was observed (Luciano et al, 2011;Dieci et al, 2013Dieci et al, , 2016Lodde et al, 2013;Rose et al, 2013;Franciosi et al, 2014;Zeng et al, 2014;Azari-Dolatabad et al, 2016;Li et al, 2016;Park et al, 2016;Sanchez et al, 2017;Soares et al, 2017). For instance, blastocysts with higher number of cells or embryo with better developmental kinetics.…”

Section: Controlling Campmentioning

confidence: 99%

The variable success of in vitro maturation: can we do better?

Luciano¹,

Franciosi²,

Barros³

et al. 2018

Anim Reprod

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The efficiency of in vitro assisted reproductive technologies, consisting of the transfer of embryos obtained in vitro through in vitro maturation, in vitro fertilization and early embryo culture is still limited. The quality of the oocytes is pivotal for assisted reproductive efficiency and the maturation of the oocyte represents the first key limiting step of the in vitro embryo production system. At the time of removal from the antral follicles, the oocyte is still completing the final growth and differentiation steps, needed to provide the so-called developmental competence, i.e. the machinery required to sustain fertilization and embryo development. In mono-ovular species only one oocyte per cycle is available for procreation, therefore the current assisted reproduction techniques strive to overcome this natural boundary. However, the success is still limited and overall the effectiveness does not exceed the efficiency achieved in millions of years of mammalian evolution. One of the problems lies in the intrinsic heterogeneity of the oocytes that are subjected to in vitro maturation and in the lack of dedicated in vitro approaches to finalize the differentiation process. In this review we will try to overview some of the salient aspects of current practices by emphasizing the most critical and fundamental features in oocyte differentiation that should be carefully considered for improving current techniques.

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“…Forskolin is an AC activator that stimulates the production of cAMP (Thomas, Thompson, Armstrong, & Gilchrist, 2004). Forskolin can increase oocyte cAMP concentrations (Park et al, 2016), maintain meiotic arrest (Albuz et al, 2010; Leal et al, 2019), improve embryonic development (Albuz et al, 2010; Park et al, 2016), increase intra‐oocyte glutathione and decrease intra‐oocyte H 2 O 2 (Li et al, 2016). The dibutyryl cAMP (dbcAMP) molecule is a membrane‐permeable analog of cAMP that mimics the function of endogenous cAMP.…”

Section: Introductionmentioning

confidence: 99%

Combined use of dbcAMP and IBMX minimizes the damage induced by a long‐term artificial meiotic arrest in mouse germinal vesicle oocytes

Han

Hao

et al. 2020

Molecular Reproduction Devel

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Phosphodiesterase (PDE)‐mediated reduction of cyclic adenosine monophosphate (cAMP) activity can initiate germinal vesicle (GV) breakdown in mammalian oocytes. It is crucial to maintain oocytes at the GV stage for a long period to analyze meiotic resumption in vitro. Meiotic resumption can be reversibly inhibited in isolated oocytes by cAMP modulator forskolin, cAMP analog dibutyryl cAMP (dbcAMP), or PDE inhibitors, milrinone (Mil), Cilostazol (CLZ), and 3‐isobutyl‐1‐methylxanthine (IBMX). However, these chemicals negatively affect oocyte development and maturation when used independently. Here, we used ICR mice to develop a model that could maintain GV‐stage arrest with minimal toxic effects on subsequent oocyte and embryonic development. We identified optimal concentrations of forskolin, dbcAMP, Mil, CLZ, IBMX, and their combinations for inhibiting oocyte meiotic resumption. Adverse effects were assessed according to subsequent development potential, including meiotic resumption after washout, first polar body extrusion, early apoptosis, double‐strand DNA breaks, mitochondrial distribution, adenosine triphosphate levels, and embryonic development. Incubation with a combination of 50.0 μM dbcAMP and 10.0 μM IBMX efficiently inhibited meiotic resumption in GV‐stage oocytes, with low toxicity on subsequent oocyte maturation and embryonic development. This work proposes a novel method with reduced toxicity to effectively arrest and maintain mouse oocytes at the GV stage.

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Cilostamide and forskolin treatment during pre-IVM improves preimplantation development of cloned embryos by influencing meiotic progression and gap junction communication in pigs

Cited by 24 publications

References 47 publications

Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes

Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes

The variable success of in vitro maturation: can we do better?

Combined use of dbcAMP and IBMX minimizes the damage induced by a long‐term artificial meiotic arrest in mouse germinal vesicle oocytes

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