Ciliary Neurotrophic Factor Role in Myelin Oligodendrocyte Glycoprotein Expression in Cuprizone-Induced Multiple Sclerosis Mice

Abstract

Multiple sclerosis (MS) is an inflammatory disease of the central nervous system that leads to loss of myelin and oligodendrocytes and damage to axons. Myelin oligodendrocyte glycoprotein (MOG) is a minor component of the myelin sheath, but is an important autoantigen linked to the pathogenesis of MS. Ciliary neurotrophic factor (CNTF) has been shown to enhance the generation, maturation, and survival of oligodendrocytes in culture medium. The aim of this study was to demonstrate the role of CNTF on MOG expression in the cerebral cortex of Cuprizone-induced MS mice. The mice were treated by Cuprizone for five weeks in order to induce MS. The mice were then divided into 3 groups. The first group was injected subcutaneously (SC) by CNTF in the amount of 250 μg/kg BW per day. The second group (SHAM) was injected SC by normal saline and the third group was left without injection as the control group. After four weeks the mice were killed and the cerebral cortex was harvested and the expression of MOG was studied by Western blotting. The data from this study show that the MOG expression was significantly increased in the CNTF-injected group as compared to the other groups. It is concluded that CNTF increases the MOG expression and may be important in the pathophysiology of MS. It is also concluded that CNTF may play a role in the process of remyelination by inducing the MOG expression.

Chronic Oligodendrogenesis and Remyelination after Spinal Cord Injury in Mice and Rats

“…Previous work using immunohistochemistry and Western blot showed that CNTF and FGF-2 are elevated for 4 wpi around the lesion border (Tripathi and McTigue, 2008); the present work extends this increased growth factor expression to 12 wpi. CNTF is an astrocyte-derived factor that promotes OL lineage cell survival, differentiation, and myelination (Barres et al, 1993(Barres et al, , 1996Mayer et al, 1994;Salehi et al, 2013;Vernerey et al, 2013). It initiates phosphorylation of STAT3 and enhances the effects of FGF-2, which is also important for oligodendrogenesis (Dell'Albani et al, 1998;Baron et al, 2000;Yokogami et al, 2000).…”

Original paper Administration of leukemia inhibitory factor increases Opalin and myelin oligodendrocyte glycoprotein expression in the cerebral cortex in a cuprizone-induced model of demyelination

“…The role of ciliary neurotrophic factor (CNTF) in the process of remyelination has been demonstrat ed [22]. Here we show that daily administration of LIF significantly increases MOG and Opalin expres sion in the cuprizoneinduced mouse cerebral cortex.…”

Administration of vitamin D 3 induces CNPase and myelin oligodendrocyte glycoprotein expression in the cerebral cortex of the murine model of cuprizone-induced demyelination

“…Growth factors including insulin like growth factor were shown to be important in repair processes of demyelination [12]. Ciliary neurotrophic factor (CNTF) and leukemia inhibitory factor (LIF) have been shown to have an important role in the process of remyelination by increasing Opalin (34 KDa) and MOG expression [23,29]. It is suggested that the activation of MOG transcription depends more on an intrinsic oligodendroglial maturation program of myelination.…”