Ciliary Activity in Differentiating and Reactivated Human Respiratory Epithelial Cells

Hard, R; Besch, Stephen R.; Tristram, Deborah; Han, Jin C.; Hicks, Wesley L.

doi:10.1097/00005537-199901000-00020

Cited by 9 publications

(6 citation statements)

References 9 publications

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“…Moreover, CBF has been utilized as an index of ciliary activity to evaluate the ciliotoxicity of nasal formulations. [4][5][6][7] Some in vitro evaluation systems on CBF, utilizing a video, photoelectric detection, or high-speed digital imaging system, have been reported, 2) where various types of cells, such as primary-cultured epithelial cells [8][9][10] and single ciliary cells, 11) were used for the evaluation of CBF. However, the relationship between MC and CBF is still quantitatively ambiguous 12) and there has been no study on the CBF of the rat nasal epithelium and its relationship with MC.…”

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confidence: 99%

<i>In Vitro</i> Evaluation of the Ciliary Beat Frequency of the Rat Nasal Epithelium Using a High-Speed Digital Imaging System

Inoue

Furubayashi

Ogawara

et al. 2013

Biological & Pharmaceutical Bulletin

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Mucociliary clearance (MC) is an important factor in determining nasal drug absorption and the ciliary beat of ciliated epithelial cells of the nasal mucosa is the driving force of MC. However, the relationship between MC and ciliary beat frequency (CBF) is still ambiguous. The purpose of this study was to establish an evaluation method of CBF as an index of mucociliary function and examine the relationship between MC and CBF. A sequence of images of ciliary beating of an excised rat nasal septum was captured using a high-speed digital video camera. CBF (beats per second, Hz) was determined from periodic changes in the contrast value of a specific location in a sequence of images. CBF under control conditions was 8.49±0.38 Hz, which is similar to values reported for cultured human nasal epithelial cells and rat tracheal cells. β-Adrenergic and cholinergic antagonists decreased CBF, while β-adrenergic agonists and acetylcholine increased CBF. These results were similar with those observed for MC in our previous study. It was found that CBFs were significantly and linearly correlated with MC, indicating that MC is directly regulated by CBF and that this evaluation system allows the quantitative determination of nasal mucociliary function.Key words mucociliary clearance; ciliary beat frequency; high-speed digital imaging method; nasal absorption; cilia; nasal cavity Nasal drug delivery has several advantages over oral drug administration. After the nasal application of drugs, a faster onset of action is expected due to no time being needed to reach the absorption site, a relatively large absorptive surface area, and high vascularization. Furthermore, it has been shown that bioavailability is also greater due to the avoidance of presystemic metabolism and/or chemical degradation.

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confidence: 99%

<i>In Vitro</i> Evaluation of the Ciliary Beat Frequency of the Rat Nasal Epithelium Using a High-Speed Digital Imaging System

Inoue

Furubayashi

Ogawara

et al. 2013

Biological & Pharmaceutical Bulletin

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“…2,4,11,26 Results of several of these studies have indicated that the intracellular signaling pathway of this reaction involves adenylate cyclase/cyclic adenosine monophosphate (cAMP)/protein kinase A, guanylate cyclase/cyclic guanosine monophosphate (cGMP)/protein kinase G, nitric oxide synthase/nitric oxide, and calmodulin. 2,4,11,26,27 The mechanism of the ciliary beat has also been investigated in cultured human airway epithelial cells, 14,[28][29][30][31][32][33] and some difference in the signaling pathway was reported between human and animals. 14 Although the use of cultured cells is generally a powerful method for research on cellular biology, it is often doubtful whether cells in culture behave in the same way as those in the tissue.…”

Section: Discussionmentioning

confidence: 99%

Acetylcholine-induced Ciliary Beat of the Human Nasal Mucosa Is Regulated by the Pannexin-1 Channel and Purinergic P2X Receptor

Ohbuchi

Wakasugi

et al. 2018

Am J Rhinol�Allergy

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These results indicate that the regulatory mechanism of the Ach-induced ciliary beat is dependent on extracellular Ca and involves the muscarinic Ach receptor, IP receptor, pannexin-1 channel, purinergic P2X receptor, and connexin channel. We proposed a tentative intracellular signaling pathway of the Ach-induced ciliary beat, in which the pannexin-1-P2X unit may play a central role in ciliary beat regulation.

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“…Various cell culture systems of NECs have been proposed and extensively studied on the ciliogenesis, ciliary movement, mucus secretion, nasal mucosal infection, and the underlying cellular and molecular mechanisms 9, 10. To date, several key factors for promoting mucociliary differentiation of NECs have been suggested, such as air‐liquid biphasic culture technique, collagen substrate, and growth‐regulation factors, e.g., retinoic acid 11, 12.…”

Section: Discussionmentioning

confidence: 99%

Culture of nasal epithelial cells using chitosan‐based membranes

et al. 2009

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Ciliary Activity in Differentiating and Reactivated Human Respiratory Epithelial Cells

Cited by 9 publications

References 9 publications

<i>In Vitro</i> Evaluation of the Ciliary Beat Frequency of the Rat Nasal Epithelium Using a High-Speed Digital Imaging System

<i>In Vitro</i> Evaluation of the Ciliary Beat Frequency of the Rat Nasal Epithelium Using a High-Speed Digital Imaging System

Acetylcholine-induced Ciliary Beat of the Human Nasal Mucosa Is Regulated by the Pannexin-1 Channel and Purinergic P2X Receptor

Culture of nasal epithelial cells using chitosan‐based membranes

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