Ciguatera risk assessment in two toxic sites of French Polynesia using the receptor-binding assay

Darius, Hélène Taiana; Ponton, Dominique; Revel, Taina; Cruchet, Philippe; Ung, André; Fouc, Mote Tchou; Chinain, Mireille

doi:10.1016/j.toxicon.2007.05.007

Cited by 95 publications

(123 citation statements)

References 46 publications

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“…Still, Seriola fasciata sample 2 weighed 37 kg and is 60 times more toxic than Seriola fasciata sample 5, which weighed 63 kg. This observation is in agreement with other works [32,33] who have reported in the Pacific (French Polynesia) no clear relation between CTXs content and the size of fish specimens. The same authors have also reported the presence of CTXs in both herbivorous and carnivorous fish specimens [34].…”

Section: Resultssupporting

confidence: 94%

Ciguatera, an Emerging Human Poisoning in Europe

Acosta¹

2015

JAMB

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Section: Resultssupporting

confidence: 94%

Ciguatera, an Emerging Human Poisoning in Europe

Acosta¹

2015

JAMB

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“…Very seldom, ciguatera intoxication or accumulation of toxins in fish were related to observable punctual events of Gambierdiscus blooms (Bagnis et al, 1985). Cell abundance and toxicity of natural populations of Gambierdiscus in fishing areas may be used as an indicator of local ciguatera risk (Chinain et al, 2010b;Darius et al, 2007). However cell density of Gambierdiscus in natural populations does not always correlate with its CTXs production.…”

Section: Introductionmentioning

confidence: 99%

“…Many methodological approaches have been used for the determination of CTXs such as bioassays with animals like the mouse bioassay (MB) (Banner et al, 1961;Lewis and Sellin, 1992;Yasumoto et al, 1984); instrumental analytical methods (Dickey et al, 1992;Legrand et al, 1989;Lewis et al, 1999Lewis et al, , 2009); immunoassays (Campora et al, 2008a(Campora et al, , 2008bHokama et al, 1998); pharmacological assays like the radio binding assay (RBA) (Darius et al, 2007;Dechraoui et al, 2005;Van Dolah et al, 1994) as well as in vitro cell based assay (CBA) (Campora et al, 2008a;Cañ ete and Diogè ne, 2010;Dechraoui et al, 2005;Dickey et al, 1999;Manger et al, 1995Manger et al, , 1993. A comprehensive update on methodologies available for ciguatoxin determination has been recently reviewed (Caillaud et al, 2010a).…”

Section: Introductionmentioning

confidence: 99%

Monitoring of dissolved ciguatoxin and maitotoxin using solid-phase adsorption toxin tracking devices: Application to Gambierdiscus pacificus in culture

et al. 2011

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“…this assay is based on the competitive binding of radio-labeled [ 3 H]-brevetoxin-3 to sodium-channels in rat brain synaptosomes in the presence of CTX-group toxins. The limit of quantification (LOQ) for this assay was reported at 0.16 µg P-Ctx-3Cequiv/kg fish (Darius et al, 2007). the approach of receptor binding assays refers to the MOA of CFP toxins and has the potential not only to detect and quantify CTX contamination but also to provide the basis for evaluation of human responsiveness to these toxins.…”

Section: Functional Biochemical and Biomolecular Assaysmentioning

confidence: 99%

A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems

Daneshian

2013

ALTEX

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Summary Aquatic food accounts for over 40% of global animal food products, and the potential contamination with toxins of algal origin -marine biotoxins -poses a health threat for consumers. The gold standards to assess toxins in aquatic food have traditionally been in vivo methodssingle marine biotoxins or combinations of marine biotoxins and intoxication symptomatology as well as monitoring the "success" of controls implemented during fishery and aquaculture production, processing, and retailing. For this reason, a workshop was organized in ermatingen, Switzerland by the Center for Alternatives to Animal testing -europe (CAAteurope) within the framework of the transatlantic think tank for toxicology (t 4 ).In contrast to other food products where hygiene and potential contamination with microbes or mold toxins is the prime issue, the emphasis in aquatic products (shellfish and finfish) is, beyond viral and bacterial contaminations, primarily placed on potential contamination with marine biotoxins owing to the numerous and recurring human intoxications.The gold standards to assess toxins in aquatic food have traditionally been in vivo methods, i.e., the mouse and the rat bioassays. Besides the ethical issues of in vivo bioassays there are specific difficulties, e.g., different exposure route (i.p. versus oral), low inter-species comparability, high intra-species variability, and questionable extrapolation of quantitative risk to humans, thus highlighting the need for the development of more reliable detection and quantification methods. Based on this reasoning, the european Food Safety Authority (eFSA) advocates the use of an analytical method, i.e., LC-MS (Liquid Chromatography coupled Mass Spectrometry), as a substitute for in vivo bioassays for almost all classes of marine toxins. However, although lC-MS is a very sensitive method that has the advantages of being able to detect multiple toxins in a single analysis and being good for confirming the identity of toxins, the quality of quantitative analysis is dependent on the availability of calibration standards. While many new toxin structural analogues have been detected and identified using LC-MS, this technique -as with most other methods -is not good at detecting new toxin types. When new toxin analogues are detected but accurate standards are not yet available, only an approximate quantitation is possible using estimated response factors.For the purpose of risk assessment of food, however, it is imperative to focus on the possible adverse effects, independent of whether they stem from one toxin or a combination of toxins. As toxicity is defined by functional and structural changes of biological systems, the development of a human-relevant in vitro system for appropriate risk assessment of marine biotoxins in seafood stands to reason. Moreover, poor correlation of human intoxications, of acute symptomatology and long-term adverse effects, and of predictive in vitro, in vivo, and analytical detection methodologies of marine biotoxins stems not least from a...

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Ciguatera risk assessment in two toxic sites of French Polynesia using the receptor-binding assay

Cited by 95 publications

References 46 publications

Ciguatera, an Emerging Human Poisoning in Europe

Ciguatera, an Emerging Human Poisoning in Europe

Monitoring of dissolved ciguatoxin and maitotoxin using solid-phase adsorption toxin tracking devices: Application to Gambierdiscus pacificus in culture

A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems

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