Cigarillos Compromise the Mucosal Barrier and Protein Expression in Airway Epithelia

Abdelwahab, Sabri H.; Reidel, Boris; Martin, Jessica R.; Ghosh, Arunava; Keating, James E.; Haridass, Prashamsha; Carpenter, Jerome; Glish, Gary L.; Tarran, Robert; Doerschuk, Claire M.; Kesımer, Mehmet

doi:10.1165/rcmb.2019-0085oc

Cited by 5 publications

(4 citation statements)

References 53 publications

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“…Among several clusters in this heatmap, the following clusters that characterized different toxicity outcomes were significant: Group I included proteins with similar increases after CBD exposure that were associated with the processes of glycan degradation and carbon metabolism (Figure S6). Exposure to CBD aerosols was associated with increases in proteins that play a role in detoxification and redox stress (Figure 10), which have also been observed in response to cigarette smoke in airway secretions in vitro and in vivo (Abdelwahab et al, 2020; A. Ghosh et al, 2017; Reidel et al, 2018). In addition, CBD exposure also led to increases in proteins that are targets and responders to natural xenobiotics (Fang et al, 2009), such as SELENBP1 (Figure 10C).…”

Section: Discussionmentioning

confidence: 83%

“…These differential patterns of aerosol composition-specific protein level changes are further illustrated in the heatmap of Group I included proteins with similar increases after CBD exposure that were associated with the processes of glycan degradation and carbon metabolism (Figure S6). Exposure to CBD aerosols was associated with increases in proteins that play a role in detoxification and redox stress (Figure 10), which have also been observed in response to cigarette smoke in airway secretions in vitro and in vivo (Abdelwahab et al, 2020;A. Ghosh et al, 2017;Reidel et al, 2018).…”

Section: Discussionmentioning

confidence: 83%

“…In previous studies, we have demonstrated that our apical airway proteomic analyses correlate well with individual proteins measurements and are a valuable tool to globally assess toxicity effects of tobacco product use on the airways (Abdelwahab et al, 2020;Ghosh et al, 2017). To further characterize the effects of CBD and VEA aerosols on the airway surface, we collected apical lining fluid samples of HBE cultures daily 2 h after exposure and analyzed the protein composition by LC-MS/MS.…”

Section: Discussionmentioning

confidence: 86%

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Vaping additives cannabinoid oil and vitamin E acetate adhere to and damage the human airway epithelium

Reidel

Abdelwahab

Wrennall

et al. 2022

J of Applied Toxicology

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E‐cigarette, or vaping product use‐associated lung injury (EVALI), is a severe respiratory disorder that caused a sudden outbreak of hospitalized young people in 2019. Using cannabis oil containing vaping products, including vitamin E acetate contaminants, was found to be strongly associated with EVALI. However, the underlying tissue impacts of the condition are still largely unknown. Here, we focused on the vehicle cannabinoid oil (CBD oil) and contaminant vitamin E acetate (VEA) effects on airway epithelial cells. Primary human bronchial epithelial (HBE) cultures were exposed to e‐liquid aerosols that contained CBD oil and VEA in combination or the common e‐liquid components PG/VG with and without nicotine. Cell viability analysis indicated dramatically increased cell death counts after 3 days of CBD exposure, and this effect was even higher after CBD + VEA exposure. Microscopic examination of the cultures revealed cannabinoid and VEA depositions on the epithelial surfaces and cannabinoid accumulation in exposed cells, followed by cell death. These observations were supported by proteomic analysis of the cell secretions that exhibited increases in known markers of airway epithelial toxicity, such as xenobiotic enzymes, factors related to oxidative stress response, and cell death indicators. Overall, our study provides insights into the association between cannabinoid oil and vitamin E acetate vaping and lung injury. Collectively, our results suggest that the adherent accumulation of CBD oil on airway surfaces and the cellular uptake of both CBD oil‐ and VEA‐containing condensates cause elevated metabolic stress, leading to increased cell death rates in human airway epithelial cultures.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 86%

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Vaping additives cannabinoid oil and vitamin E acetate adhere to and damage the human airway epithelium

Reidel

Abdelwahab

Wrennall

et al. 2022

J of Applied Toxicology

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“…Prolonged exposure to tobacco not only affects the respiratory tract and airway epithelial cells but also destroys their barrier-like function and ultimately blocks airflow by increasing goblet cell and submucosal glands and their secretions (25)(26)(27).…”

Section: Effects Of Tobacco On Different Body Systemsmentioning

confidence: 99%

Association Between Tobacco Use and COVID-19: A Narrative Review

et al. 2022

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The coronavirus, which leads to coronavirus disease 19 (COVID-19), may affect various systems of the body (including respiratory, heart, blood circulation, gastrointestinal, nervous, urinary, and renal systems). Similar to COVID-19, first- and second-hand smoking also endanger the immune system and affect a wide range of systems. Therefore, considering the adverse effects of tobacco on different systems of the body, this study aimed at investigating the effects of tobacco on the onset, transmission, prognosis, and severity of COVID-19 in patients. Related articles were selected through advanced searching in Scopus, PubMed, and ScienceDirect databases and Google search engine while including no time limit and using three keywords derived from Mesh (COVID-19, smoking, and tobacco). Most studies have indicated that the incidence of COVID-19 was extremely lower in patients with a history of active smoking compared to patients with no smoking history. According to the results, smoking has an impact on the prognosis and outcome of COVID-19, increases the severity of the disease, the need for hospitalization and the intensive care unit (ICU), and the death rate, as well as challenging the treatment process and changing the attitude of smokers, enhancing their desire to quit smoking. Therefore, smokers are less likely to develop COVID-19, but the rate of transmission, disease severity, and mortality and the need for hospitalization increase in these subjects, and thus treatment will be challenging. Thus, quitting smoking may help improve the function of the respiratory system.

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Airway mucin MUC5AC and MUC5B concentrations and the initiation and progression of chronic obstructive pulmonary disease: an analysis of the SPIROMICS cohort

Radicioni

Ceppe

Ford

et al. 2021

The Lancet Respiratory Medicine

106

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Cigarillos Compromise the Mucosal Barrier and Protein Expression in Airway Epithelia

Cited by 5 publications

References 53 publications

Vaping additives cannabinoid oil and vitamin E acetate adhere to and damage the human airway epithelium

Vaping additives cannabinoid oil and vitamin E acetate adhere to and damage the human airway epithelium

Association Between Tobacco Use and COVID-19: A Narrative Review

Airway mucin MUC5AC and MUC5B concentrations and the initiation and progression of chronic obstructive pulmonary disease: an analysis of the SPIROMICS cohort

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