Cigarette Smoking Induces Formation of 8-Hydroxydeoxyguanosine, One of the Oxidative Dna Damages in Human Peripheral Leukocytes

Abstract

Active oxygen species (AOS) such as O2- and H2O2 have been shown to be generated from both gas and tar phases of cigarette smoke and it has been suggested that they are involved in carcinogenesis due to cigarette smoking. Therefore, we investigated the effect of cigarette smoking on oxidative DNA damages in human peripheral blood cells using 8-hydroxydeoxy-guanosine (8-OH-dG) as a marker. From ten healthy male volunteers aged 20-22 years, 5 ml of blood was taken before and 10 minutes after smoking 2 cigarettes in 10 minutes. After lysis of blood cell membranes leukocyte DNA was isolated using a DNA extractor and 8-OH-dG levels were determined using high performance liquid chromatography (HPLC) with electrochemical detection. The mean levels of 8-OH-dG increased significantly (P less than 0.05) from 3.3 +/- 0.8/10(6) dG (mean +/- SD) to 5.1 +/- 2.5 after smoking. These results indicate that cigarette smoking induces oxidative DNA damage in peripheral blood cells in a relatively short time.

Alterations of the tear film and ocular surface health in chronic smokers

“…Fume particles and noxious gases such as nicotine and carbon monoxide may influence the circulatory system by inducing profound vasospasm, platelet aggregation, or leading to oxidative damage to lipids, proteins, and cellular DNA. [2][3][4][5][6][7][8] Epidemiological data link cigarette smoking as a risk factor for many ophthalmological disorders including strabismus in offspring of pregnant smokers, anterior ischaemic optic neuropathy, age related macular degeneration, cataract, thyroid ophthalmopathy, primary open angle glaucoma, diabetic retinopathy, uveal melanoma, conjunctival intraepithelial neoplasia, ocular sarcoidosis, and development of dry eyes. [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] The Beaver Dam Study related current active smoking and past history of smoking to a higher prevalence of dry eye disease.…”

Associação entre funções da via auditiva eferente e genotoxicidade em adultos jovens

“…Other authors 30 have also investigated the effect of smoking on oxidative damage to DNA in peripheral blood cells. These authors used 8-hydroxydeoxyguanosine (8-OH-dG) as a marker.…”

The measurement of urinary 8-hidroxy-2'deoxyguanosine level with liquid chromatography-tandem mass spectrometry method in patients with polycystic ovary syndrome

“…The observation of nearly seven-times more differences in 8-OHdG levels obtained from leukocytes DNA of nonsmokers leads to idea that genetic factors and lifestyle may have effect on the increase of 8-OHdG level. According to some studies, age factor, smoking, and working hours in jobs carrying threats of cancer risk goes parallel with the increase of urinary 8-OHdG level (26) . In our study, we have found a positive (r = 0.42, p <0.05) difference between 8-OHdG/creatinine ratio and smoke in PCOS patients but we have not found any significant difference between PCOS patients with smokers and non-smokers in terms of 8-OHdG/creatinine ratio.…”