Cigarette smoke condensate alters Saccharomyces cerevisiae efflux transporter mRNA and activity and increases caffeine toxicity

Effects of Ca2+ signal on the activities of key enzymes and expression of related genes in yeast ethanol metabolism and mitochondrial function during high sugar fermentation

“…At 6 and 24 h, the mitochondrial membrane potential of yeast in the high‐glucose‐with‐Ca 2+ group was significantly higher than that of the other two experimental groups, indicating that the polarization phenomenon of yeast in a high sugar environment was more significant in the presence of Ca 2+ , which also explains the higher content of ROS in the yeast cells of the high‐glucose‐with‐Ca 2+ group 44 . In addition, the mitochondrial membrane potential is a necessary presence to maintain the growth and metabolism of various cells; the significant increase in the mitochondrial membrane potential of yeast in the high‐glucose‐with Ca 2+ ‐group at 6 and 24 h suggests that the incorporation of Ca 2+ may have further enhanced the respiratory metabolism capacity of the yeast 45 . Therefore, the addition of Ca 2+ in a high sugar environment can increase the mitochondrial membrane potential of yeast more effectively, and yeast in the high‐glucose‐with‐Ca 2+ group may possess a higher energy‐yielding capacity.…”

“…44 In addition, the mitochondrial membrane potential is a necessary presence to maintain the growth and metabolism of various cells; the significant increase in the mitochondrial membrane potential of yeast in the high-glucose-with Ca 2+ -group at 6 and 24 h suggests that the incorporation of Ca 2+ may have further enhanced the respiratory metabolism capacity of the yeast. 45 Therefore, the addition of Ca 2+ in a high sugar environment can increase the mitochondrial membrane potential of yeast more effectively, and yeast in the high-glucose-with-Ca 2+ group may possess a higher energy-yielding capacity.…”

Cigarette smoke interacts with amiodarone toxicity in Saccharomyces cerevisiae

“…S. cerevisiae strains (Table 1) were supplied by Euroscarf (Oberursel, Germany). They were cultured as previously described (Sayyed et al, 2019) and treated with AMD ± CSC (or BaP, or Phe), and growth was monitored by optical density measurement (600 nm) after 6 and 24 h. The 50% inhibitory concentrations IC 50 and confidence intervals were calculated, and the statistical analysis performed as previously described (Sayyed et al, 2018).…”

“…AMD idiosyncratic toxicity may depend on genetic and environmental factors such as exposure to cigarette smoke. Several models, including Saccharomyces cerevisiae (S. cerevisiae), are valid to investigate drug toxicity and to identify potential interactions with pollutants such as cigarette smoke (Al-Attrache et al, 2018;Pena et al, 2009;Sayyed et al, 2018Sayyed et al, , 2019. AMD causes oxidative stress and induces relevant genes (e.g., catalase CTT1) in S. cerevisiae (Zhang & Rao, 2007).…”

Saccharomyces cerevisiae and Caffeine Implications on the Eukaryotic Cell

“…The pleiotropic effect of caffeine on S. cerevisiae can be used to develop a model to study the toxic effects of various substances [ 30 ]. It was found that caffeine toxicity is enhanced in yeast cells following exposure to cigarette smoke and that yeast efflux transporters are targets of cigarette smoke chemicals, suggesting once more that associating caffeine-rich products with smoking is not recommended [ 31 ]. In line with habitual behavior studies and considering that most of the caffeine beverages are consumed hot (tea, coffee, chocolate), it was revealed that associating caffeine exposure with hyperthermia had an increased mutagenic effect on S. cerevisiae cells when pure caffeine was used [ 32 ].…”