Cigarette Smoke Affects Apoptosis in Rat Tongue Mucosa: Role of bcl-2 Gene Family

Assis, Gérson Francisco de; Ceolin, Daniele S.; Marques, Sílvio Alencar; Salvadori, Daisy Maria Fávero; Ribeiro, Daniel Araki

doi:10.1007/s10735-006-9023-z

Cited by 26 publications

(29 citation statements)

References 41 publications

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“…Very little is known about the possible impact of tobacco smoke on the apoptosis of epithelial cells in the oral cavity, and observations vary, from indicating no changes on the level of apoptosis [30] to suggesting increased levels of hypodiploid cells [31] in the epithelium of smokers through the inhibition of apoptosis under influence of nicotine [20,32]. However, these observations are based on in vitro cultured keratinocytes and animal models.…”

Section: Introductionmentioning

confidence: 99%

Tobacco smoking alters the number of oral epithelial cells with apoptotic features

Michcik

Cichorek

Daca

et al. 2014

Folia Histochem Cytobiol.

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Tobacco smoking is a global problem associated with the occurrence of many systemic diseases and tumors. Oral cavity tumors are common tobacco-related cancers, and of all the anatomical structures that are exposed to the effects of smoking, the oral cavity remains the least-explored area. Changes that occur in the biology of oral epithelial keratinocytes under the influence of the components of tobacco smoke often go unnoticed, if they are asymptomatic. The proper functioning of the oral epithelium is determined by the proliferation and differentiation of the cells in keratinization -the process of programmed cell death, which extends through to the mechanisms of apoptosis. Due to incomplete knowledge of the impact of tobacco smoke on the biology of keratinocytes, an evaluation of the cell cycle was conducted and the apoptosis of oral epithelial keratinocytes was analyzed. The study involved 77 patients divided into four groups according to their intensity of smoking, ranging from 0 to 27 pack-years. There were no differences in the cell count between nonsmokers and smokers in the proper cell-cycle phases. The percentage of proliferating cells in the oral epithelium is about 11%. A reduction in the number of early-apoptotic cells (caspase positive/propidium iodide negative) and an increase in the number of late-apoptotic cells (caspase positive/annexin V positive/propidium iodide positive) were observed to occur with increasing pack-years. The present study demonstrates that smoking does not affect the oral keratinocyte cell cycle, but does modify the number of cells with early and late apoptotic features. An intensification of the impact of tobacco smoke components on the biology of the oral keratinocytes is clearly noticeable at approximately 6 pack-years. This indicates that the biology of the first organ exposed to tobacco smoke -the oral epithelium -is altered by tobacco smoking.

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Section: Introductionmentioning

confidence: 99%

Tobacco smoking alters the number of oral epithelial cells with apoptotic features

Michcik

Cichorek

Daca

et al. 2014

Folia Histochem Cytobiol.

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“…25 DNA mutations are detected in tumours closely associated with smoking, such as those located in the oral, oropharyngeal and lung cavities. 26 In particular, studies of epithelial tissue from smokers have shown elevated levels of DNA damage and increased DNA mutations compared with epithelial tissue from non-smokers. 27 Considering this, it would be interesting to know whether or not, and to what extent, heavy smokers are a more susceptible group following X-ray exposure in distinct sites of oral cavity, particularly because there are no previous reports.…”

Section: Resultsmentioning

confidence: 99%

Cytogenetic biomonitoring in oral mucosa cells following dental X-ray

Ribeiro¹

2012

Dentomaxillofacial Radiology

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Objectives: In the past decades, X-rays have been used widely for diagnosis in dentistry. However, it is well known that ionizing radiation causes damage (including single-and double-strand breaks) to deoxyribonucleic acid (DNA) and DNA-protein crosslinks, and induces cellular death. Therefore, outlining the cytogenetic effects induced by X-ray is necessary to identify the degree of cancer risk and minimize potential risks to patients and clinicians. To date, a variety of assays have been proposed in cytogenetic biomonitoring studies, including those that assess metaphase chromosomal aberrations and sister chromatid exchanges, and micronucleus and single-cell gel (comet) assay. Methods: Cytogenetic biomonitoring studies focusing on oral mucosa cells in individuals exposed to dental X-ray were reviewed. Results: Dental X-ray can induce DNA damage and cytotoxicity in oral mucosa cells. Conclusion: These results will contribute to a better understanding of X-ray-induced effects upon the cellular system in individuals continually exposed to known genotoxic/cytotoxic agents.

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“…However, it is important to estimate the real health risks as well as the severity of adverse effects induced by cigarette smoke in oral mucosa prior to clinically detectable symptoms in order to more precisely assess the role of tobacco into the development of oral neoplasms. Our own recent results have demonstrated that cigarette smoke is able to interfere with apoptosis in rat tongue mucosa cells (Assis et al 2005).…”

Section: Introductionmentioning

confidence: 94%

Expression of placental glutathione S-transferase in rat tongue mucosa exposed to cigarette smoke

Ribeiro

Assis²

2007

J Mol Hist

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Glutatione S-transferases (GSTs) are a family of enzymes involved in detoxification of xenobiotics. Placental GST, known as GST-P, has been detected in tissues following exposure to carcinogenic agents being regarded a reliable biomarker of exposure and susceptibility in early phases of carcinogenesis. The aim of this study was to investigate the expressivity of GST-P positive foci in the rat tongue mucosa exposed to cigarette smoke by means of immunohistochemistry. A total of twelve male Wistar rats were distributed into two groups: negative control and experimental group exposed to cigarette smoke during 75 days. After experimental period, no histopathological changes in the tongue mucosa were evidenced in the negative control and the experimental group. However, a total of five GST-P positive foci were detected in two out of six animals exposed to cigarrette smoke. None control animals were noticed GST-P positive foci. These data indicate that expression of GST-P may reflect the carcinogenic effect of cigarette smoke as well as the genetic susceptibility of animals in relation to continuous carcinogens exposure.

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Cigarette Smoke Affects Apoptosis in Rat Tongue Mucosa: Role of bcl-2 Gene Family

Cited by 26 publications

References 41 publications

Tobacco smoking alters the number of oral epithelial cells with apoptotic features

Tobacco smoking alters the number of oral epithelial cells with apoptotic features

Cytogenetic biomonitoring in oral mucosa cells following dental X-ray

Expression of placental glutathione S-transferase in rat tongue mucosa exposed to cigarette smoke

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