Çiğ Süt ve Süt Ürünlerinde Listeria monocytogenes Varlığı ve Antibiyotik Direnci

Abstract

How to Cite This ArticleAksoy A, Sezer Ç, Vatansever L, Gülbaz G: Presence and antibiotic resistance of Listeria monocytogenes in raw milk and dairy products . Kafkas Univ Vet Fak Derg, 24 (3): 415-421, 2018. AbstractIn this study, the presence of Listeria monocytogenes in raw milk and milk products produced from raw milk by traditional method, such as cheese and butter using cultural and molecular techniques and the antibiotic resistances were investigated. Isolation was performed using the FDA technique. Microbact 12L test kit was used for biochemical identification. Genotyping of isolates was done by traditional PCR method. Antibiotic resistance of isolates was determined by standard disk diffusion method. Listeria spp. was detected in 16 (5.3%) of the total 300 food samples according to the biochemical identification results. The highest prevalence of Listeria spp. was in raw milk samples (10%), followed by cheese samples (4%) and butter samples (2%). All 36 Listeria spp. isolates which were obtained from biochemical identification were tested for L. monocytogenes by PCR, and 15 (41.6%) of them were confirmed as L. monocytogenes. According to the antibiotic resistance test results of L. monocytogenes strains, four (26.7%) isolates were resistant to at least one antibiotic while only one (6.7%) had multiple antibiotic resistance. The highest resistance was found against trimethoprim-sulfamethoxazole. It has been determined that L. monocytogenes isolates had the same degree resistance against penicillin G, meropenem, amikacin and vancomycin. In conclusion, the presence of L. monocytogenes which has been detected in dairy products made from raw milk and in raw milk itself may constitute a potential risk for public health. Çiğ Süt ve Süt Ürünlerinde Listeria monocytogenes Varlığı ve Antibiyotik DirenciÖz Bu çalışmada, çiğ süt ve geleneksel yöntemlerle çiğ sütten üretilen süt ürünlerinde (peynir ve tereyağ) kültürel ve moleküler teknikler kullanılarak Listeria monocytogenes varlığı ve antibiyotik direnci araştırıldı. İzolasyon FDA tekniğine göre yapıldı. Biyokimyasal identifikasyon için Microbact 12L test kiti kullanıldı. İzolatların genetik olarak tanımlanması klasik PCR yöntemi ile yapıldı. İzolatların antibiyotik direnci standart disk difüzyon yöntemi ile belirlendi. Biyokimyasal identifikasyon sonuçlarına göre toplamda 300 gıda örneğinin 16'sında (5.3%) Listeria spp. tespit edildi. Listeria spp. en yüksek çiğ süt örneklerinde bulunurken (%10), bunu peynir örnekleri (%4) ve (%2) tereyağı örnekleri izledi. Biyokimyasal test sonuçlarına göre elde edilen 36 adet Listeria spp. izolatının tümü L monocytogenes yönünden PCR ile test edildi ve 15'i (%41.6) L. monocytogenes olarak doğrulandı. L monocytogenes izolatlarının antibiyotik direnç test sonuçlarında izolatların dördü (%26.7) en az bir antibiyotiğe dirençli iken biri (%6.7) çoklu antibiyotik dirence sahipti. En yüksek direnç trimetoprim-sülfametoksazole karşı bulundu. L. monocytogenes izolatlarının penisilin G, meropenem, amikasin ve vankomisine karşı aynı ora...

Microbiological Assessment of Dairy Products Produced by Small-Scale Dairy Producers in Serbia

“…Resistance to TRS has also been seen in other studies [26,106]. Since this antibiotic is used in listeriosis treatment, the finding of TRS resistance in food chain isolates is concerning [107][108][109]. An earlier study from France reported an overall high rate of susceptibility of clinical L. monocytogenes strains to a range of antibiotics, including those used in listeriosis treatment [31].…”

Determination of the presence and antibiotic resistance of listeria species and aerobic mesophilic bacteria count of cow milks

“…Today, antibiotic resistance is a wide spreading issue addressed by the World Health Organization (WHO). Aksoy et al (1) stated that they found intermediate and resistant to amikacin, meropenem, vancomycin and penicillin G, and Trimethoprim-Sulfamethoxazole in 15 L. monocytogenes isolates they isolated from raw cow milk, cheese and butter. Jamali and Redmehr (23) stated resistance against penicillin G, amoxicillin-clavulanic acid, chloramphenicol, erythromycin, and tetracycline at the rates of 66.7%, 23.8%, 19%, 4.8% and 52.4%, respectively, in 17 L. monocytogenes isolates they have isolated from the clinical mastitis infections of cows.…”

“…After incubation, the inhibition zones were measured on Mueller-Hingon F agar plates and evaluated as resistant (R), intermediate (I) and susceptible (S) based on the breakpoints suggested by EUCAST (18) for L. monocytogenes on ampicillin, penicillin G, oxacillin, erythromycin, trimethoprim-sulfamethoxazole, meropenem and for the other antibiotic discs commented based on the breakpoints suggested by CLSI ( 14) for other Gram-positive bacteria (1,13,14,18). Escherichia coli (ATCC ® 25922™) were used as reference on antibitoic susceptability tests.…”