Chylomicrons of Blood: A Resumé of Literature and a Recommended Counting Procedure

Frailing, Jean M.; Owen, Clarence I.

doi:10.1093/ajcp/21.6.508

Cited by 8 publications

(2 citation statements)

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“…In 1946, Chargaff and West [6] isolated the coagulation components of the platelet-free plasma by high-speed centrifugation (31,000× g), but it was not clear whether these structures were the same as Haemokonien. Other names describing blood serum particles such as protein particles, fat particles, fat dust, disintegrated platelets, chylomicrons, or white blood cells were also introduced [7]. In 1967, Wolf introduced the term "platelet-dust" to distinguish the platelet-secreted coagulant-material-carrying particles from chylomicrons.…”

Section: Introductionmentioning

confidence: 99%

The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation

Kaddour

Tranquille

Okeoma

2021

Viruses

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Extracellular vesicles (EVs) are cell-derived membranous particles secreted by all cell types (including virus infected and uninfected cells) into the extracellular milieu. EVs carry, protect, and transport a wide array of bioactive cargoes to recipient/target cells. EVs regulate physiological and pathophysiological processes in recipient cells and are important in therapeutics/drug delivery. Despite these great attributes of EVs, an efficient protocol for EV separation from biofluids is lacking. Numerous techniques have been adapted for the separation of EVs with size exclusion chromatography (SEC)-based methods being the most promising. Here, we review the SEC protocols used for EV separation, and discuss opportunities for significant improvements, such as the development of novel particle purification liquid chromatography (PPLC) system capable of tandem purification and characterization of biological and synthetic particles with near-single vesicle resolution. Finally, we identify future perspectives and current issues to make PPLC a tool capable of providing a unified, automated, adaptable, yet simple and affordable particle separation resource.

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Section: Introductionmentioning

confidence: 99%

The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation

Kaddour

Tranquille

Okeoma

2021

Viruses

View full text Add to dashboard Cite

show abstract

“…In 1946, Chargaff and West [5] isolated the coagulation components of the platelet-free plasma by high-speed centrifugation (31,000×g), but it wasn't clear whether these structures were the same as Haemokonien. Other names describing blood serum particles such as protein particles, fat particles, fat dust, disintegrated platelets, chylomicrons, or white blood cells were also introduced [6]. In 1967, Wolf introduced the term "platelet-dust" to distinguish the platelet-secreted coagulant-material-carrying particles from chylomicrons.…”

Section: Introductionmentioning

confidence: 99%

The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation

Kaddour¹,

Tranquille²,

Okeoma³

2021

Preprint

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Extracellular vesicles (EVs) are cell-derived membranous particles secreted by all cell types into the extracellular milieu. EVs carry, protect, and transport a wide array of bioactive cargoes to recipient/target cells. EVs regulate physiological and pathophysiological processes in recipient cells and are important in therapeutics/drug delivery. Despite these great attributes of EVs, an efficient protocol for EV separation from biofluids is lacking. Numerous techniques have been adapted for the separation of EVs with size exclusion chromatography (SEC)-based methods being the most promising. Here, we review the SEC protocols used for EV separation, and discuss opportunities for significant improvements, such as the development of novel particle purification liquid chromatography (PPLC) system capable of tandem purification and characterization of biological and synthetic particles with near-single vesicle resolution. Finally, we identify future perspectives and current issues to make PPLC a tool capable of providing a unified, automated, adaptable, yet simple and affordable particle separation resource.

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Recent Advances in Nutrition and Metabolism

Goldsmith¹

1952

AMA Arch Intern Med

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Chylomicrons of Blood: A Resumé of Literature and a Recommended Counting Procedure

Cited by 8 publications

References 0 publications

The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation

The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation

The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation

Recent Advances in Nutrition and Metabolism

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