Chylomicron remnant induction of lipid accumulation in J774 macrophages is associated with up-regulation of triacylglycerol synthesis which is not dependent on oxidation of the particles

Napolitano, Mariarosaria; Avella, Michael; Botham, Kathleen M.; Bravo, Elena

doi:10.1016/s1388-1981(03)00042-8

Cited by 46 publications

(47 citation statements)

References 43 publications

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“…The amount of [ 3 H]-oleate incorporated into triglycerides was determined as previously described [31]. Cells were incubated for 3 h at 37°C in serum-free medium containing 6 µCi/ml [ 3 H]-oleate bound to fatty acid-free bovine serum albumin (BSA; 2%).…”

Section: Methodsmentioning

confidence: 99%

Pomegranate Juice Protects Macrophages from Triglyceride Accumulation: Inhibitory Effect on DGAT1 Activity and on Triglyceride Biosynthesis

Rosenblat

Aviram²

2011

Ann Nutr Metab

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Background/Aims: To analyze the effects of pomegranate juice (PJ) and punicalagin on macrophage triglyceride metabolism. Methods: Triglyceride metabolism was analyzed in PJ- or punicalagin-treated J774A.1 macrophages or in mouse peritoneal macrophages (MPM) harvested from C57BL/6 mice or from paraoxonase 2 (PON2)-deficient mice. Results: PJ (0–50 µM) significantly and dose-dependently decreased the triglyceride content and triglyceride biosynthesis rate in J774A.1 macrophages or in C57BL/6 MPM by about 30%. Similarly, punicalagin, the major PJ polyphenol, inhibited the MPM triglyceride biosynthesis rate by 40%. The triglyceride hydrolytic rate, however, was not significantly affected by PJ or punicalagin. The activity of diacylglycerol acyltransferase 1 (DGAT1; the rate-limiting enzyme in triglyceride biosynthesis) was significantly inhibited, by 54%, in C57BL/6 MPM that were treated with 50 µM PJ or punicalagin, with no significant effect on DGAT1 mRNA or protein expression. Both PJ and punicalagin increased (1.7-fold) MPM PON2 mRNA expression, and PON2 was previously shown to inhibit DGAT1 activity. However, the addition of PJ or punicalagin (50 µM) to microsomes from PON2-deficient MPM still resulted in a significant reduction (50–58%) in DGAT1 activity. Conclusions: We conclude that the inhibitory effect of PJ on triglyceride biosynthesis could be attributed to a direct effect of PJ on DGAT1 activity.

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Section: Methodsmentioning

confidence: 99%

Pomegranate Juice Protects Macrophages from Triglyceride Accumulation: Inhibitory Effect on DGAT1 Activity and on Triglyceride Biosynthesis

Rosenblat

Aviram²

2011

Ann Nutr Metab

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“…Triglyceride-rich lipoproteins (TRL) can cross the endothelial barrier and enter into the vascular wall [2], where they can enhance lipid accumulation into macrophages, leading to foam cell formation [3]. TRL consist of chylomicrons, which are secreted by the small intestine and contain apolipoprotein (apo) B-48 as the structural protein, and VLDL, originated in the liver and containing apo B-100.…”

Section: Introductionmentioning

confidence: 99%

Minor components of olive oil facilitate the triglyceride clearance from postprandial lipoproteins in a polarity-dependent manner in healthy men

Cabello-Moruno¹,

Martı́nez-Force²,

Montero³

et al. 2014

Nutrition Research

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were isolated from serum at 2, 4 and 6 hours postprandially and their fatty acid and TG molecular species compositions were analyzed by gas chromatography. The apolipoprotein B concentration was determined by immunoturbidimetry. POMACE and OLIVE, differing mainly in their unsaponifiable fraction, led to similar fatty acid and TG molecular species profiles in postprandial TRL. However, POMACE-TRL presented a higher particle size, estimated as TG to apolipoprotein B ratio, which was also found for the main TG molecular species (trioleoyl-glycerol, palmitoyl-dioleoyl-glycerol, palmitoyl-oeloyl-linoleoyl-glycerol, and dioleoyl-linoleoyl-glycerol). TG from POMACE-TRL also showed higher clearance rates. In this regard, apolar TG (with a higher equivalent carbon number) disappeared more rapidly from TRL particles obtained after the ingestion of either POMACE and OLIVE. In conclusion, minor components of POMACE facilitated TG clearance from TRL by modifying their particle size and the hydrolysis of the most apolar species.

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“…In contrast to other lipoproteins that accumulate in the arterial intima, CMr and VLDLr may be atherogenic in either native or oxidized form [10,11]. After the recruitment of circulating monocytes into the intima and their transformation into macrophages, these cells take up native lipoproteins via the LDL receptor and oxidized lipoproteins via scavenger receptors (SRs) [12].…”

Section: Introductionmentioning

confidence: 99%

Quantification of major lipid classes in human triacylglycerol‐rich lipoproteins by high‐performance liquid chromatography with evaporative light‐scattering detection

Perona

Ruı́z-Gutı́errez²

2004

J of Separation Science

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Triacylglycerol-rich lipoproteins (TRL), comprising chylomicrons (CM) and very-low-density lipoproteins (VLDL), have been associated with cardiovascular disease. The lipid class content in the remnant particles of these lipoproteins is a determinant for the accumulation of lipids in macrophages and their transformation into foam cells. We have optimized a method for the simultaneous determination of cholesteryl esters (CE), triacylglycerols (TG), free cholesterol (FC), monoacylglycerols (MG), and phospholipids (PL) by HPLC coupled to a light-scattering detector (ELSD). A diol column and a ternary gradient of hexane, 2-propanol, and methanol were applied to CM and VLDL of human origin (n = 10), with excellent precision in terms of repeatability of peak areas and retention times. All peaks were baseline resolved although the resolution of CE and TG was compromised for the sake of simplicity of the solvent gradient. The ELSD response was fitted to second-order equations, with correlation coefficients (r2) higher than 0.999 for a wide range of concentrations (0.25-10 microg of lipid injected). TG were the major lipid class detected in human TRL, accounting for 62% in CM obtained 2 h after the oil intake. In addition we recorded a depletion of TG and CE in CM obtained 2 h after the oil intake of about 60%. We conclude that the method reported here is suitable for a rapid and precise determination of lipid classes in human TRL and, therefore, may be a useful tool for investigations on the atherogenicity of these lipoproteins.

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Chylomicron remnant induction of lipid accumulation in J774 macrophages is associated with up-regulation of triacylglycerol synthesis which is not dependent on oxidation of the particles

Cited by 46 publications

References 43 publications

Pomegranate Juice Protects Macrophages from Triglyceride Accumulation: Inhibitory Effect on DGAT1 Activity and on Triglyceride Biosynthesis

Pomegranate Juice Protects Macrophages from Triglyceride Accumulation: Inhibitory Effect on DGAT1 Activity and on Triglyceride Biosynthesis

Minor components of olive oil facilitate the triglyceride clearance from postprandial lipoproteins in a polarity-dependent manner in healthy men

Quantification of major lipid classes in human triacylglycerol‐rich lipoproteins by high‐performance liquid chromatography with evaporative light‐scattering detection

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