Chthonobacter albigriseus gen. nov., sp. nov., isolated from grass-field soil

Abstract

A novel aerobic, Gram-stain-negative and non-motile bacterial strain, designated ED7 T , was isolated from grass-field soil in Cheonan, Korea. Strain ED7 T utilized methanol and methylamine, but not formate, as carbon and energy sources. The strain was able to grow at 20-42 C (optimum 30-35 C), at pH 7.0-8.5 (optimum pH 7.5-8.0), and in the absence of NaCl. According to the similarities of the 16S rRNA gene sequences, strain ED7 T was most closely related to the genera Labrenzia ( 93.3 % 16S rRNA gene sequence similarity), Pleomorphomonas ( 93.1 %) and Prosthecomicrobium ( 93.1 %). A phylogenetic analysis based on the 16S rRNA gene sequence of strain ED7 T revealed that it was affiliated with the family Methylocystaceae, being most closely related to the genus Pleomorphomonas. In contrast to Pleomorphomonas koreensis and Pleomorphomonas oryzae, strain ED7 T did not contain the nifH gene. The DNA G+C content of the genomic DNA of strain ED7 T was 71.8 mol%.The predominant fatty acids of strain ED7 T were summed feature 8 (C 18 : 1 !7c and/or C 18 : 1 !6c), summed feature 2 (C 14 : 0 3-OH, and/or C 16 : 1 iso I), 11-methyl C 18 : 1 !7c and C 16 : 0 3-OH. The major isoprenoid quinone was ubiquinone 10 (Q-10). The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unknown aminophospholipid. Based on phenotypic, chemotaxonomic and genotypic characteristic data, strain ED7 T could be differentiated from other genera, suggesting that strain ED7 T represents a novel species of a new genus in the family Methylocystaceae, for which the name Chthonobacter albigriseus gen. nov., sp. nov. is proposed. The type strain of the type species is ED7 T (=JCM 30603 T =KCTC 42450 T ).

Faunimonas pinastri gen. nov., sp. nov., an endophyte from a pine tree of the family Pleomorphomonadaceae, class Alphaproteobacteria

“…The order Hyphomicrobiales includes 38 families, and the family Pleomorphomonadaceae includes the genera Chthonobacter , Hartmannibacter , Methylobrevis , Mongoliimonas , Oharaeibacter and Pleomorphomonas (https://lpsn.dsmz.de/family/pleomorphomonadaceae, accessed on 1 March 2022, and from ‘List of new names and new combinations previously effectively, but not validly, published’ issues of this journal). Members of this family have been isolated from grass-field soil [1], rhizosphere soil [2–4], desert soil [5], paddy soil [6], anaerobic sludge [7], crude cultures [8], a contaminated culture of the phototrophic bacterium Rhodopseudomonas palustris [9] and saline hot spring [10]. Most of these genera stain Gram-negative and are rod-shaped, and their major respiratory lipoquinones are ubiquinones 10 (Q-10).…”

Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales (Rhizobiales)

“…Physiological and biochemical characteristics and enzyme activities were tested at 25 • C using the API 20NE and APIZYM test strips (bioMérieux, Tokyo, Japan) according to the manufacturer's instructions. Growth on methanol was tested with a minimal medium K containing methanol (0.5%, v/v) as reported in a previous study [23]. Nitrogen fixation potential was assessed based on the growth at 25 • C for 3-4 weeks on a nitrogen-free MS2N medium following the method of Bao et al [24].…”

Methylobrevis albus sp. nov., isolated from freshwater lake sediment

“…The genus Methylobrevis is comprised of Gram-stainnegative, strictly aerobic bacteria who do not form endospores or prosthesis. Up to now only one described strain, M. pamukkalensis (Doronina et al 2015), has been con rmed to belong to the genus Methylobrevis within the family Pleomorphomonadaceae, whichwas recently revised with genus Chthonobacter (Kim et al 2017), Hartmannibacter (Suarez et al 2014), Methylobrevis (Doronina et al 2015), Mongoliimonas(Jiao et al 2017), Oharaeibacter (Lv et al 2017) and Pleomorphomonas (Xie and C.-H. 2005) supposed as the only six type strains base on LPSN (LPSN, List of Prokaryotic Names with Standing in Nomenclature)(Parte) (Hrdt et al 2020). In October 2019, we isolated the strain L22 T from freshwater of Hulun Lake, Inner Mongolia, China.…”