Chrysotila pseudoroscoffensis as a source of high-value polar lipids with antioxidant activity: A lipidomic approach

Moreira, Ana S. P.; Gonçalves, Joana; Conde, Tiago; Couto, Daniela; Melo, Tânia; Maia, Inês B.; Pereira, Hugo; Silva, Joana; Domingues, M. Rosário M.; Nunes, Cláudia

doi:10.1016/j.algal.2022.102756

Cited by 9 publications

(2 citation statements)

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“…Diacylglyceryl‐N,N,N‐trimethyl‐homoserine is the main betaine lipid found in green algal membranes (Li‐Beisson et al, 2019), while DGTA and DGCC have been reported in some algal species. Diacylglycerylhydroxymethyl‐N,N,N‐trimethyl‐β‐alanine is one common constituent of brown algae (Dembitsky, 1996; Lopes, Melo, et al, 2021), and DGCC is frequently identified in the lipidome of haptophyte microalgae (Aveiro et al, 2020; Moreira, Gonçalves, et al, 2022). Several studies have confirmed the role of betaine lipids in the lipid remodeling system occurring during phosphorus limitation (Murakami et al, 2018; Oishi et al, 2022).…”

Section: Resultsmentioning

confidence: 99%

“…Pigments have strong antioxidant properties by avoiding light damage and protecting the cell against reactive oxygen species. Several studies have demonstrated the antioxidant activity of algal lipid extracts (Moreira, Gonçalves, et al, 2022; Rey et al, 2020; Santos et al, 2020). However, systematic bioactivity studies using isolated lipid fractions or lipid species are scarce in the literature (Conde et al, 2022; Lopes, Rey, et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

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Revealing the polar lipidome, pigment profiles, and antioxidant activity of the giant unicellular green alga, Acetabularia acetabulum

Rey

Cartaxana

Cruz

et al. 2023

Journal of Phycology

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Marine algae are one of the most important sources of high‐value compounds such as polar lipids, omega‐3 fatty acids, photosynthetic pigments, or secondary metabolites with interesting features for different niche markets. Acetabularia acetabulum is a macroscopic green single‐celled alga, with a single nucleus hosted in the rhizoid. This alga is one of the most studied dasycladalean species and represents an important model system in cell biology studies. However, its lipidome and pigment profile have been overlooked. Total lipid extracts were analyzed using hydrophilic interaction liquid chromatography‐high resolution mass spectrometry (HILIC‐HRMS), tandem mass spectrometry (MS/MS), and high‐performance liquid chromatography (HPLC). The antioxidant capacity of lipid extracts was tested using DPPH and ABTS assays. Lipidomics identified 16 polar lipid classes, corresponding to glycolipids, betaine lipids, phospholipids, and sphingolipids, with a total of 191 lipid species, some of them recognized by their bioactivities. The most abundant polar lipids were glycolipids. Lipid classes less studied in algae were identified, such as diacylglyceryl‐carboxyhydroxymethylcholine (DGCC) or hexosylceramide (HexCer). The pigment profile of A. acetabulum comprised carotenoids (17.19%), namely cis‐neoxanthin, violaxanthin, lutein and β,β‐carotene, and chlorophylls a and b (82.81%). A. acetabulum lipid extracts showed high antioxidant activity promoting a 50% inhibition (IC50) with concentrations of 57.91 ± 1.20 μg · mL−1 (438.18 ± 8.95 μmol Trolox · g−1 lipid) in DPPH and 20.55 ± 0.60 μg · mL−1 in ABTS assays (918.56 ± 27.55 μmol Trolox · g−1 lipid). This study demonstrates the potential of A. acetabulum as a source of natural bioactive molecules and antioxidant compounds.

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