Chrysin suppresses LPS-stimulated proinflammatory responses by blocking NF-κB and JNK activations in microglia cells

Ha, Sang Keun; Moon, Eunjung; Kim, Sun Yeou

doi:10.1016/j.neulet.2010.08.064

Cited by 149 publications

(80 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, LPS activates not only NF-NB, which is a pivotal transcription factor for inflammatory gene expression, but also induces phosphorylation of mitogen-activated protein kinases (MAPKs), which in turn leads to the activation of NF-NB in macrophages (Jung et al, 2008;Ha et al, 2010). MAPKs are signalling molecules that play critical roles in the regulation of cell growth, differentiation, cell survival/apoptosis, cellular response to cytokines, stress and inflammation.…”

Section: Introductionmentioning

confidence: 99%

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages

Búfalo

Ferreira

Costa

et al. 2013

Journal of Ethnopharmacology

148

View full text Add to dashboard Cite

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages, Journal of Ethnopharmacology, http://dx.doi.org/ 10. 1016/j.jep.2013.06.004 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractEthnopharmacological relevance: Propolis is a bee product with numerous biological and pharmacological properties, such as immunomodulatory and anti-inflammatory activities.It has been used in folk medicine as a healthy drink and in food to improve health and prevent inflammatory diseases. However, little is known about its mechanism of action.Thus, the goal of this study was to verify the antioxidant activity and to explore the antiinflammatory properties of propolis by addressing its intracellular mechanism of action.Caffeic acid was investigated as a possible compound responsible for propolis action. Materials and Methods:The antioxidant properties of propolis and caffeic acid were evaluated by using the 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) scavenging method. To analyze the anti-inflammatory activity, Raw 264.7 macrophages were treated with different concentrations of propolis or caffeic acid, and nitric oxide (NO) production, a strong pro-inflammatory mediator, was evaluated by the Griess reaction. The concentrations of propolis and caffeic acid that inhibited NO production were evaluated on intracellular signaling pathways triggered during inflammation, namely p38 mitogenactivated protein kinase (MAPK), c-jun NH 2 -terminal kinase (JNK1/2), the transcription nuclear factor (NF)-NB and extracellular signal-regulated kinase (ERK1/2), through Western blot using specific antibodies. A possible effect of propolis on the cytotoxicity of hepatocytes was also evaluated, since this product can be used in human diets.Results: Caffeic acid showed a higher antioxidant activity than propolis extract. Propolis and caffeic acid inhibited NO production in macrophages, at concentrations without cytotoxicity. Furthermore, both propolis and caffeic acid suppressed LPS-induced signaling pathways, namely p38 MAPK, JNK1/2 and NF-NB. ERK1/2 was not affected by propolis extract and caffeic acid. In addition, propolis and caffeic acid did not induce hepatotoxicity at concentrations with strong anti-inflammatory potential. Conclusions:Propolis exerted an antioxidant and anti-inflammatory action and caffeic acid may be involved in its inhibitory effects on NO production and intracellular signaling cascades, suggesting its use as a natural source of safe anti-inflammatory drugs.

show abstract

Section: Introductionmentioning

confidence: 99%

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages

Búfalo

Ferreira

Costa

et al. 2013

Journal of Ethnopharmacology

148

View full text Add to dashboard Cite

show abstract

“…It would be interesting, therefore, to search for molecules that could help control inflammation in the CNS [7][8][9][10]. Natural polyphenols have been shown to exert neuroprotective properties by inhibiting the release of proinflammatory cytokines after LPS-activation of microglia [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Obtaining from Grape Pomace an Enzymatic Extract with Anti-inflammatory Properties

Rodríguez-Morgado¹,

Candiracci

Santa-María³

et al. 2014

Plant Foods Hum Nutr

View full text Add to dashboard Cite

Grape pomace, a winemaking industry by-product, is a rich source of bioactive dietary compounds. Using proteases we have developed an enzymatic process for obtaining a water-soluble extract (GP-EE) that contains biomolecules such as peptides, carbohydrates, lipids and polyphenols in soluble form. Of especial interest is its high polyphenol content (12 %), of which 77 % are flavonoids and 33 % are phenolic acids. The present study evaluates in vitro the potential anti-inflammatory effect of GP-EE by monitoring the expression of inflammatory molecules on N13 microglia cells stimulated with lipopolysaccharide (LPS). GP-EE decreases the mRNA levels of the inflammatory molecules studied. The molecules under study were as follows: inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), the ionized calcium binding adaptor molecule-1(Iba-1) and the Toll like receptor-4 (TLR-4), as well as the iNOS protein level in LPS-stimulated microglia. Our findings suggest that, as a result of its ability to regulate excessive microglial activation, GP-EE possesses antiinflammatory properties. Therefore, acting as a chemopreventive agent, it may be of therapeutic interest in neurodegenerative diseases involving neuroinflammation. We can, therefore, propose GP-EE as a useful natural extract and one that would be beneficial to apply in the field of functional foods.

show abstract

“…The reduction of COX2 by chrysin is due to its inhibitory effects on the activities of several transcription factors that regulate COX2 gene transcription in different tissues. Chrysin is able to inhibit the activity of NF-kB and reduce COX2 promoter activity (Ha et al, 2010;Li et al, 2010). In macrophages, chrysin suppressed lipopolysaccharide (LPS)-induced COX2 expression by inhibiting activity of nuclear factor for IL-6 (NF-IL6) (Woo et al, 2005).…”

Section: Chrysinmentioning

confidence: 99%

“…Another member of C/EBP family is C/EBP that is activated by c-Jun N terminal kinase (JNK) (Cho et al, 2003). Chrysin inhibited JNK activation and reduced LPS-induced COX2 expression (Ha et al, 2010). In addition, the cytokines, such as IL-1 and TNF-, are able to induce COX2 expression (Chen et al, 2001a;Ishikawa et al, 2005).…”

Section: Chrysinmentioning

confidence: 99%

“…In addition, the cytokines, such as IL-1 and TNF-, are able to induce COX2 expression (Chen et al, 2001a;Ishikawa et al, 2005). It was observed that chrysin significantly reduced the LPS-released IL-1 and TNF- (Ha et al, 2010;Romier et al, 2008). In addition to the inhibition of COX2 gene expression, chrysin and its derivatives are able to inhibit COX2 activity.…”

Section: Chrysinmentioning

confidence: 99%

See 1 more Smart Citation

Natural Flavonoids in StAR Gene Expression and Testosterone Biosynthesis in Leydig Cell Aging

Wang¹

2011

Basic and Clinical Endocrinology Up-to-Date

View full text Add to dashboard Cite

Chrysin suppresses LPS-stimulated proinflammatory responses by blocking NF-κB and JNK activations in microglia cells

Cited by 149 publications

References 38 publications

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages

Obtaining from Grape Pomace an Enzymatic Extract with Anti-inflammatory Properties

Natural Flavonoids in StAR Gene Expression and Testosterone Biosynthesis in Leydig Cell Aging

Contact Info

Product

Resources

About