Chryseobacterium pennipullorum sp. nov., isolated from poultry feather waste

Abstract

Strain 7_F195T was previously isolated from chicken feather waste collected from an abattoir in Bloemfontein, South Africa. A polyphasic approach was followed to determine if strain 7_F195T belongs to the genus Chryseobacterium and if the organism can be classified as a new species. The nearest neighbours, based on 16S rRNA gene sequence similarity values (indicated in parentheses), were Chryseobacterium flavum KCTC 12877T (98.42 %), Chryseobacterium indologenes LMG 8337T (98.24 %) and Chryseobacterium gleum ATCC 35910T (97.71 %). Genome sequencing revealed a genome size of 4 796 535 bp and a DNA G+C content of 38.6 mol%. The ANI values of strain 7_F195T compared to C. flavum , C. indologenes and C. gleum were 81.45, 81.86 and 82.38 %, respectively. The digital DNA–DNA hybridization values for strain 7_F195T with C. flavum , C. indologenes and C. gleum were 23.7, 23.7 and 24.9 %, respectively. Notable phenotypic differences include the presence of urease activity in C. indologenes LMG 8337T and C. gleum NCTC 11432T, but not in strain 7_F195T or C. flavum KCTC 12877T. The predominant fatty acids of strain 7_F195T were iso-C15 : 0, iso-C17 : 1 ω9c and iso-C17 : 0 3-OH and the most abundant polar lipid was phosphatidylethanolamine. Menaquinone-6 was the only respiratory quinone. Based on the data generated from this polyphasic study, strain 7_F195T represents a novel Chryseobacterium species for which the name Chryseobacterium pennipullorum sp. nov. is proposed. The type strain is 7_F195T (=LMG 30781T=KCTC 62760T).

Corrigendum: Correction to manuscripts describing the fatty acid composition of organisms submitted to IJSEM between 2014 and 2021

“…The previously published articles listed in the References [1–50] contained the phrase ‘Summed features are groups of two or three fatty acids that are treated together for the purpose of evaluation in the MIDI system and include both peaks with discrete ECLs as well as those where the ECLs are not reported separately’, which was taken from and consequently should have cited the following article:…”

Chryseobacterium pennae sp. nov., isolated from poultry feather waste

“… Chryseobacterium species have been isolated from a variety of food sources including fish, meat, poultry and dairy products [6–11]. Some species cause spoilage in milk due to lipolytic and proteolytic activities [12].…”

“…The genome of strain 1_F178 T was sequenced and assembled as described by Oosthuizen et al . [ 11 ]. Assembly of reads resulted in 88 contigs with a total sequence length of 6.18 Mbp and a G+C content of 36.6 mol%.…”

“…Phenotypic and biochemical tests were carried out as reported by Oosthuizen et al . [ 11 ]. Strains were cultivated in NB at 25 °C for 48 h. The cells were centrifuged at 3000 g for 10 min (Eppendorf).…”

Chryseobacterium vaccae sp. nov., isolated from raw cow's milk

“…At the time of writing (October 2019), the genus Chryseobacterium contains 113 validly published species (http://www.bacterio.net/chryseobacterium.html) with Chryseobacterium gleum as the type species. These many species of the genus have been isolated from a variety of environmental sources such as soil, rhizosphere, freshwater, water of a reservoir, wetland, plants, and animal sources including raw cow’s milk, chicken, poultry feathers, fish and clinical samples [2–14]. The general characteristics of the genus Chryseobacterium are as follows: Gram-stain negative, aerobic, rod-shaped, non-motile, non-spore-forming and yellow-pigmented.…”