Chrysanthemum virus B and chrysanthemum chlorotic mottle viroid infect chrysanthemum in South Africa

Abstract

This is the first report of chrysanthemum virus B (CVB) and chrysanthemum chlorotic mottle viroid (CChMVd) on Chrysanthemum × morifolium cultivars, in South Africa. Samples were collected from plants showing vein clearing, mosaic and chlorosis, in the Western Cape province. RNAtag-seq was applied and indicated the presence of CVB in all four samples and the co-infection with CChMVd in one sample. The presence of CVB and CChMVd were confirmed using RT-PCR and Sanger sequencing.