Chrysanthemum indicum Linné extract inhibits the inflammatory response by suppressing NF-κB and MAPKs activation in lipopolysaccharide-induced RAW 264.7 macrophages

Cheon, Myeong Sook; Yoon, Taesook; Lee, Doyeon; Choi, Goya; Moon, Byeong Cheol; Lee, A Yeong; Choo, Byung-Kil; Kim, Ho Kyoung

doi:10.1016/j.jep.2009.01.034

Cited by 86 publications

(64 citation statements)

References 28 publications

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“…In order to investigate the anti-inflammatory effects on LPS-induced RAW 264.7 macrophage cells, CHI extract as a whole plant was used in this study. Recent studies reported that CHI extract significantly inhibited the expression of pro-inflammatory cytokines but most of the previous studies were used a specific part of CHI such as roots, stem, leaf and flower, which was extracted using an ethanol [18,19,21]. There was no study using whole plant extracts of chrysanthemum with water.…”

Section: Discussionmentioning

confidence: 99%

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Anti-Inflammatory Effects of Chrysanthemum indicum Water Extract in RAW 264.7 Cell as a Whole Plant

Kang¹

2015

Journal of Korean Biological Nursing Science

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Purpose: Chrysanthemum indicum (CHI) has been used for edible and medical purposes for a long time in Korea. The purpose of this study was to evaluate the anti-inflammatory effects of CHI water extract in lipopolysaccharides (LPS)-induced RAW 264.7 macrophage cells. Methods: To investigate the anti-inflammatory effects on LPS-induced RAW 264.7 macrophage cells, CHI extract as a whole plant was used in this study. RAW 264.7 cells were treated with various concentrations of CHI extract (1, 10, and100 μg/mL). After that Nitric Oxide (NO), inducible nitric oxide synthase (iNOS), interleukin (IL)-1β, cyclooxygenase (COX)-2 and prostaglandin E2 (PGE2) expression level were measured. Results: CHI extract significantly suppressed the LPS-induced NO production and decreased the level of iNOS, IL-1β, COX-2 messenger ribonucleic acid (mRNA) expression and also the down regulation of PGE2 expression in a dose-dependent manner. Conclusion: The present study suggested that CHI extract can be substituted for anti-inflammatory drugs and provide a safe and effective non pharmacological therapeutic approach. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Key Words: Anti-inflammation; Chrysanthemum indicum; RAW 264.7 cell

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Section: Discussionmentioning

confidence: 99%

“…Recent studies have demonstrated that CHI has high anti-inflammatory and antioxidant effect through the down-regulation of IL, TNF-α expression. But most of the previous studies were used a specific part of a compositae plant such as stem, leaf and flower, which were extracted using an ethanol [8,[16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Effects of Chrysanthemum indicum Water Extract in RAW 264.7 Cell as a Whole Plant

Kang¹

2015

Journal of Korean Biological Nursing Science

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“…CBMFF is known to have various biological properties including anticancer, inhibition of angiogenesis, and antiinflammation Lee et al, 2003;Perry, 1980). Flower extract of Chrysanthemum indicum Linné, which belongs to the same genus as CBM, exhibited inhibitory activity on inflammation and proliferation in various cells (Cheon et al, 2009;Li et al, 2009;Yuan et al, 2009). In the present study, we found that CBMFF contained components such as thymol and eugenol that can exert anticancer, antioxidant, antimicrobial, anti-inflammation, and vasorelaxant effect (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…It is reported that the flower extract of Chrysanthemum indicum inhibits the activation of MAPKs including p38 MAPK in macrophages (Cheon et al, 2009), although this is a different type of cell. Based on these results, it is possible that CBMFF may exhibit inhibitory activity on RASMC migration and proliferation in response to PDGF-BB, probably via the suppression of p38 MAPK phosphorylation.…”

Section: Discussionmentioning

confidence: 99%

Chrysanthemum borealeflower floral water inhibits platelet-derived growth factor-stimulated migration and proliferation in vascular smooth muscle cells

Kim

Won

Yoon

et al. 2014

Pharmaceutical Biology

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(2015) Chrysanthemumboreale flower floral water inhibits platelet-derived growth factor-stimulated migration and proliferation in vascular smooth muscle cells, Pharmaceutical Biology, 53:5, 725-734, DOI: 10.3109/13880209.2014 Context: Chrysanthemum boreale Makino (Compositae) (CBM) is a traditional medicine that has been used for the prevention or treatment of various disorders; it has various properties including antioxidation, anti-inflammation, and antitumor. Objective: The present study was designed to explore the in vitro effect of CBM flower floral water (CBMFF) on atherosclerosis-related responses in rat aortic smooth muscle cells (RASMCs). Materials and methods: CBMFF was extracted from CBM flower by steam distillation and analyzed using gas chromatography-mass spectrometry. The anti-atherosclerosis activity of CBMFF was tested by estimating platelet-derived growth factor (PDGF)-BB (10 ng/mL)-induced proliferation and migration levels and intracellular kinase pathways in RASMCs at CBMFF concentrations of 0.01-100 lM and analyzing ex vivo aortic ring assay. Results: Gas chromatography-mass spectrometry showed that the CBMFF contained a total of seven components. The CBMFF inhibits PDGF-BB-stimulated RASMC migration and proliferation (IC 50 : 0.010 lg/mL). Treatment of RASMCs with PDGF-BB induced PDGFR-b phosphorylation and increased the phosphorylations of MAPK p38 and ERK1/2. CBMFF addition prevented PDGF-BBinduced phosphorylation of these kinases (IC 50 : 008 and 0.018 lg/mL, for p38 MAPK and ERK1/ 2, respectively), as well as PDGFR-b (IC 50 : 0.046 lg/mL). Treatment with inhibitors of PDGFR, P38 MAPK, and ERK1/2 decreased PDGF-BB-increased migration and proliferation in RASMCs. Moreover, the CBMFF suppressed PDGF-BB-increased sprout outgrowth of aortic rings (IC 50 : 0.047 lg/mL). Discussion and conclusion: These results demonstrate that CBMFF may inhibit PDGF-BB-induced vascular migration and proliferation, most likely through inhibition of the PDGFR-b-mediated MAPK pathway; therefore, the CBMFF may be promising candidate for the development of herbal remedies for vascular disorders.

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“…The Compositae plant, flowers of Chrysanthemum indicum (Chrysanthemi Indici Flos), one of the TCM and medicinal plants, is widely used in the treatment of hypertension, colitis, pneumonia and carbuncle in China. Mounting evidence indicates that Chrysanthemum indicum possesses antiinflammatory, antioxidant, immunomodulation and antimicrobial activities (14,15). Accumulating evidence shows that Chrysanthemum indicum is capable of inhibiting proliferation of human PC3, HL 60 and HeLa cancer cells in a dose-and time-dependent manner (16).…”

Section: Chrysanthemum Indicum Ethanolic Extract Inhibits Invasion Ofmentioning

confidence: 99%

Chrysanthemum indicum ethanolic extract inhibits invasion of hepatocellular carcinoma via regulation of MMP/TIMP balance as therapeutic target

Wang

Huang²,

Li³

et al. 2009

Oncol Rep

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Abstract. Hepatocellular carcinoma (HCC) is an aggressive cancer with a dismal outcome largely due to metastasis and postsurgical recurrence. Thus, the inhibition of invasion and metastasis is of great importance in its therapies. Medicinal plants or ethnopharmacology used in folklore medicine continue to be an important source of discovery and development of novel or potential therapeutic agents for treatment of cancer. Chrysanthemum indicum, one of the medicinal plants or ethnopharmacology, is being used for treatment of many diseases including cancer. However, this plant molecular mechanisms underlining the anti-metastatic effects have not been well documented. In this study, Chrysanthemum indicum ethanolic extract (CIE) significantly suppressed proliferation and invasion of MHCC97H cells, one of the HCC cell lines with high metastatic potential, in a dose-dependent manner. CIE markedly decreased MMP-2 and MMP-9 expression, increased simultaneously TIMP-1, and TIMP-2 expression further restoring their balance in the cancer cells. The present study indicates that CIE reduced MHCC97H cell metastatic capability, in part at least, through decrease of the MMP expression, simultaneous increase of the TIMP expression, further restoring their balance as therapeutic target in HCC. It is suggested that Chrysanthemum indicum is a potential novel therapeutic medicinal plant for treatment of HCC or cancer invasion and metastasis.

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Chrysanthemum indicum Linné extract inhibits the inflammatory response by suppressing NF-κB and MAPKs activation in lipopolysaccharide-induced RAW 264.7 macrophages

Cited by 86 publications

References 28 publications

Anti-Inflammatory Effects of Chrysanthemum indicum Water Extract in RAW 264.7 Cell as a Whole Plant

Anti-Inflammatory Effects of Chrysanthemum indicum Water Extract in RAW 264.7 Cell as a Whole Plant

Chrysanthemum borealeflower floral water inhibits platelet-derived growth factor-stimulated migration and proliferation in vascular smooth muscle cells

Chrysanthemum indicum ethanolic extract inhibits invasion of hepatocellular carcinoma via regulation of MMP/TIMP balance as therapeutic target

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