Chrysanthemum extract attenuates hepatotoxicity via inhibiting oxidative stress in vivo and in vitro

Abstract

Background ‘ Bianliang ziyu ’, a famous chrysanthemum variety commonly planted in Kaifeng, China, is often consumed by local residents. However, the hepatoprotective effects of Bianliang ziyu and their underlying mechanisms are not clear. Objective In this study, we investigated the hepatoprotective and antioxidative effects of Bianliang ziyu extract (BZE) on liver injury and explored its molecular mechanisms. Design Sprague-Dawley rats were administered BZE by intragastric administration for 8–9 days, and then alcohol or carbon tetrachloride (CCl 4 ) was administered by gavage to induce acute liver injury. The activities of serum alanine aminotransferase, aspartate aminotransferase, superoxide dismutase, and malondialdehyde in the rats were measured, and the liver of each rat was examined for histopathological changes. In vitro , HL-7702 cells were pretreated with BZE for 24 h and then exposed to 30 mmol•L −1 acetaminophen (APAP) for 12 h. The survival rate of the cells and the alanine aminotransferase and aspartate aminotransferase activities were determined. Then, we investigated the effects of BZE on oxidative stress, apoptosis, and the activation of nuclear factor erythroid-2-related factor 2 (Nrf2) signaling in HL-7702 cells induced by APAP. Results The results showed that BZE prevented alcohol-, CCl 4 -, and APAP-induced liver injury and suppressed hepatic oxidative stress in vitro and in vivo . BZE was also observed to significantly inhibit the reduction of mitochondrial membrane potential and regulate the expression of Bcl-2, Bax and Caspase-3 in APAP-induced HL-7702 cells. In addition, BZE significantly promoted nuclear translocation and the expression of Nrf2 as well as its downstream gene hemeoxygenase-1 (HO-1) in vitro . Furthermore, the findings showed that Nrf2 siRNA reversed the effects of BZE on cell survival and apoptosis-related protein expression in APAP-induced HL-7702 cells. Conclusions BZE plays an important role in preventing hepatotoxicity by inhibiting oxidative stress and apoptosis through activation of Nrf2 signaling. BZE could be developed as an effective functional food for protecting the liver.

Chrysanthemum morifolium Flower Extract Inhibits Adipogenesis of 3T3-L1 Cells via AMPK/SIRT1 Pathway Activation

“…C. zawadskii extracts inhibited adipogenesis by suppressing the expression of transcriptional factors genes in 3T3-L1 adipocytes [27,28]. Chrysanthemum morifolium is reported to exert a variety of bioactive effects associated with its major components, notably flavonoids and phenolic acids [15][16][17]29]. Nevertheless, information on the anti-obesity effect of C. morifolium remains insufficient.…”

“…It contains major components, such as luteolin, apigenin, and chlorogenic acid [ 11 ], which have been shown to exhibit anti-obesity effects by suppressing weight gain [ 12 , 13 , 14 ]. In particular, extracts of C. morifolium Ramat flower (CF) have demonstrated bioactive properties, including anti-oxidant, anti-diabetic, and hypolipidemic effects [ 11 , 15 , 16 , 17 ], but few studies have evaluated its anti-obesity effects. In this study, we aim to investigate the anti-adipogenic effect of the hot water extract of CF (HCF) in 3T3-L1 adipocytes, and to understand its underlying regulatory mechanisms by focusing on AMPK/SIRT1 activation.…”

Chrysanthemi Flosextract alleviated acetaminophen-induced rat liver injury via inhibiting oxidative stress and apoptosis based on network pharmacology analysis

“…BZE was prepared in accordance with previously developed method by our laboratory (Tian Z et al. 2019 ). Briefly, the flower of Bianliang ziyu was dried and smashed, and the powder was refluxed with 40 volume of 70% ethanol at 80 °C for 2 h. After filtration, the extracting solution was concentrated by rotary evaporator.…”

“…The doses were chosen according to our previous studies (Tian Z et al. 2019 ). The rats in the APAP + BZE groups were treated with BZE for eight consecutive days (i.g.).…”

“…Our previous studies have demonstrated that the ethanol extract of Bianliang ziyu flower (BZE) exerted protective effects in alcohol and CCl 4 -induced liver injury models (Tian et al. 2019 ). However, the detailed mechanism of BZE in liver protection remains to be illustrated.…”