Chronological histological findings of cultured epidermal autograft over bilayer artificial dermis

Matsumura, Hitoshi; Gondo, Masahide; Imai, Ryutaro; Dai, Sheng; Watanabe, Katsueki

doi:10.1016/j.burns.2012.10.004

Cited by 27 publications

(17 citation statements)

References 15 publications

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“…During the culture process, keratinocytes and fibroblasts synthesize dermal-epidermal junction proteins on either a plastic or fibrin matrix. The use of a fibrin matrix presents the advantage of conserving these proteins [22], which are otherwise cleaved by enzymatic treatment during CEA detachment from the culture vessel [50], as confirmed in our study (Fig. 5B).…”

Section: Discussionsupporting

confidence: 82%

Bioengineering a Human Plasma-Based Epidermal Substitute With Efficient Grafting Capacity and High Content in Clonogenic Cells

Alexaline

Trouillas

Nivet

et al. 2015

Stem Cells Translational Medicine

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Cultured epithelial autografts (CEAs) produced from a small, healthy skin biopsy represent a lifesaving surgical technique in cases of full-thickness skin burn covering >50% of total body surface area. CEAs also present numerous drawbacks, among them the use of animal proteins and cells, the high fragility of keratinocyte sheets, and the immaturity of the dermal-epidermal junction, leading to heavy cosmetic and functional sequelae. To overcome these weaknesses, we developed a human plasma-based epidermal substitute (hPBES) for epidermal coverage in cases of massive burn, as an alternative to traditional CEA, and set up critical quality controls for preclinical and clinical studies. In this study, phenotypical analyses in conjunction with functional assays (clonal analysis, long-term culture, or in vivo graft) showed that our new substitute fulfills the biological requirements for epidermal regeneration. hPBES keratinocytes showed high potential for cell proliferation and subsequent differentiation similar to healthy skin compared with a well-known reference material, as ascertained by a combination of quality controls. This work highlights the importance of integrating relevant multiparameter quality controls into the bioengineering of new skin substitutes before they reach clinical development. STEM CELLS TRANSLATIONAL MEDICINE 2015;4:643-654 SIGNIFICANCEThis work involves the development of a new bioengineered epidermal substitute with pertinent functional quality controls. The novelty of this work is based on this quality approach.

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Section: Discussionsupporting

confidence: 82%

Bioengineering a Human Plasma-Based Epidermal Substitute With Efficient Grafting Capacity and High Content in Clonogenic Cells

Alexaline

Trouillas

Nivet

et al. 2015

Stem Cells Translational Medicine

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“…In a variant of the original procedure cultured epithelial autografts (rather than disaggregated KC) were grafted onto DRT. Although newly synthesized epidermis and dermis showed almost normal histological appearance, formation of BM proteins was delayed; however, at a later time of observation, the newly synthesized skin was very durable and the two tissue layers could not be separated (stripped) even though they still lacked certain BM proteins (Matsumura et al 2013). …”

Section: Fig 58mentioning

confidence: 99%

Tissue and Organ Regeneration in Adults

Yannas

2015

107

100

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“…In our study, we showed that the use of both fibrin matrices allowed the deposition and the preservation of Laminin 332, Collagen IV, and Perlecan, whereas none of these proteins were present on epidermal substitutes with no matrix after dispase detachment at the time of grafting (Figure d). The enzymatic step is not solely causing the absence of these proteins as only Laminin 332 and Perlecan were expressed at a low level in epidermal substitutes before enzymatic treatment (Figure a; Matsumura, Gondo, Imai, Shibata, & Watanabe, ). Moreover, hPBM‐ and FPF‐released factors slightly enhanced Laminin 332 and Perlecan in epidermal substitutes with no matrix (Figure d‐f).…”

Section: Discussionmentioning

confidence: 99%

Influence of fibrin matrices and their released factors on epidermal substitute phenotype and engraftment

Alexaline

Magne

Rodríguez

et al. 2019

J Tissue Eng Regen Med

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SUMMARY Cultured epithelial autografts (CEAs) represent a life‐saving surgical technique for full‐thickness skin burns covering more than 60% total body surface area. However, CEAs present numerous drawbacks leading to heavy cosmetic and functional sequelae. In our previous study, we showed that human plasma‐based fibrin matrices (hPBM) could improve the reparative potential of CEAs. Therefore, in the present work, we sought to investigate the role of hPBM compared with fibrin from purified fibrinogen (FPF) or plastic support on epidermal substitute formation and engraftment. The use of hPBM for epidermal substitute culture improved keratinocyte migration, proliferation, and epidermal substitute organization to a better extent than FPF in vitro. Both fibrin matrices favored greater dermal–epidermal junction protein deposition and prevented their degradation. Keratinocyte differentiation was also decreased using both fibrin matrices. Basement membrane protein deposition was mainly influenced by matrix whereas growth factors released from fibrin especially by hPBM were shown to enhance in vitro keratinocyte migration, proliferation, and epidermal substitute organization. Ultimately, epidermal substitutes grown on hPBM displayed better engraftment rates than those cultured on FPF or on plastic support in a NOD‐SCID model of acute wound with the formation of a functional dermal–epidermal junction. Together, these results show the positive impact of fibrin matrices and their released growth factor on epidermal substitute phenotype and grafting efficiency. Fibrin matrices, and especially hPBM, may therefore be of interest to favor the treatment of full‐thickness burn patients.

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Chronological histological findings of cultured epidermal autograft over bilayer artificial dermis

Cited by 27 publications

References 15 publications

Bioengineering a Human Plasma-Based Epidermal Substitute With Efficient Grafting Capacity and High Content in Clonogenic Cells

Bioengineering a Human Plasma-Based Epidermal Substitute With Efficient Grafting Capacity and High Content in Clonogenic Cells

Tissue and Organ Regeneration in Adults

Influence of fibrin matrices and their released factors on epidermal substitute phenotype and engraftment

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