Chronologic Changes of Nitric Oxide Concentration in the Cochlear Lateral Wall and Its Role in Noise‐Induced Permanent Threshold Shift

Chen, Yuh‐Shyang; Tseng, Fen‐Yu; Lin, Kai‐Wei; Yang, Ting‐Hua; Lin‐Shiau, Shoei‐Yn; Hsu, Chuan‐Jen

doi:10.1097/mlg.0b013e3181651c24

Cited by 8 publications

(7 citation statements)

References 21 publications

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“…We need to prove this finding by checking NOS expression in these subjects in the future planning studies. We have no idea about the controversial findings on NO levels when we compare the other studies like Chen et al 22 , which they found increased NO levels after 40 hours noise exposure in guinea pigs. The longevity of the exposure may be the main factor on the level changes of NO or the other factors we do not know yet.…”

Section: Discussionmentioning

confidence: 71%

The Effects of Noise on Oxidative and Antioxidative Balance in Human Erythrocytes

Pınar¹,

Atlı²,

Alaçam³

et al. 2011

UHOD

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Reactive oxygen and nitrogen species have been implicated in the pathogenesis of noise-induced hearing loss. In this case-control study, we investigated the oxidative and antioxidative status of erythrocytes from workers in noisy workplace. Blood samples of 127 workers in noisy workplace (WNW) and 117 workers in non-noisy workplace (WNNW) from the same company were taken into tubes with potassium EDTA as anticoagulant in order to obtain hemolysate. Total superoxide dismutase (SOD) and catalase (CAT) activities as the enzymes of antioxidative defense mechanism in the erythrocytes together with malondialdehyde (MDA) as the lipid peroxidation index and total nitric oxide (NO) as an index for nitrogen species analyses were performed by spectrophotometric methods. SOD activity was found to be 450.0±106.4 U/g Hb in WNW and 443.1±83.1 U/g Hb in WNNW. The difference between two groups were not statistically significant (p= 0.582). CAT activity was found to be 426.0±98.0 k/g Hb in WNW and 432.6±109.0 k/g Hb in WNNW showing statistically insignificant difference (p= 0.621). MDA levels in erythrocytes from WNW was significantly higher than WNNW (39.28±10.22 nmol/g Hb and 32.51±10.73 nmol/g Hb, respectively and p= 0.0001). On the other hand, NO levels were found to be significantly reduced in WNW (0.275±0.187 µmol/g Hb) compared to WNNW (0.382±0.284 µmol/g Hb) (p= 0.001). When we analyzed the hematological parameters, all the cell counts increased in WNW except monocytes and platelets compared to WNNW (p= 0.0001). Related to this changes, hemoglobin, MCHC, and hematocrit also increased in WNW (p= 0.0001). The oxidative stress, which is possibly propagated by the physical environment, seems to have an important pathophysiological role in hearing loss and lipid peroxidative cellular changes in all of the workers who work in noisy occupations.Key words: Noise, Worker, Oxidative stress, Superoxide dismutase, Catalase, Malondialdehyde, Nitric oxide ÖZET Gürültünün ‹nsan Eritrositlerindeki Oksidan ve Antioksidan Dengeye EtkileriReaktif oksijen ve nitrojen türleri, yüksek ses kaynakl› duyma kay›plar›nda önemli bir patolojik faktör olarak kabul görmekte-dir. Bu vaka kontrollü çal›flmam›zda yüksek sesli ortamlarda çal›flan iflçilerin eritrositlerinde oksidan ve antioksidan dengeyi araflt›rd›k. Yüksek sesli ortamda çal›flan (YSOÇ) 127 iflçi ile normal ortamda çal›flan (NOÇ) 117 iflçinin kanlar› hemolizat elde etmek üzere içinde antikoagulan olarak potasyum EDTA bulunan tüplere al›nd›.

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Section: Discussionmentioning

confidence: 71%

The Effects of Noise on Oxidative and Antioxidative Balance in Human Erythrocytes

Pınar¹,

Atlı²,

Alaçam³

et al. 2011

UHOD

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“…As stated earlier, evidence for noise-induced generation of ROS/NO in the cochlea comes from the findings that noise exposure increases the levels of hydroxyl radicals in cochlear fluids (2), superoxide anion radicals (24), and glutathione in the stria vascularis (25) and NO in the cochlear lateral wall (26). Previous studies on NOS in the cochlea demonstrated that whereas much less NOS activity is observed in the organ of Corti (27), nNOS (NOS1) and eNOS (NOS3) are located constitutively in the modiolar core and lateral wall, respectively (28).…”

Section: Discussionmentioning

confidence: 99%

Mechanism Underlying the Protective Effect of Tempol and Nω-Nitro-L-arginine Methyl Ester on Acoustic Injury: Possible Involvement of c-Jun N-Terminal Kinase Pathway and Connexin26 in the Cochlear Spiral Ligament

et al. 2010

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Abstract. There is evidence that reactive oxygen species (ROS) are formed in the cochlea during acoustic injury. However, very little is known about the involvement of ROS signals in the spiral ligament (SL) during such injury. The purpose of this study was to determine the effect of the multifunctional antioxidant tempol and the nitric oxide synthase inhibitor N ω -nitro-L-arginine methyl ester (L-NAME) on acoustic injury and the c-Jun N-terminal kinase (JNK) pathway in the SL. Exposure of adult mice to noise (8-kHz octave band, 110-dB SPL for 1 h) produced permanent hearing loss. Noise exposure increased not only the formation of a protein modified by 4-hydroxynonenal and formation of nitrotyrosine, but also the level of phospho-JNK in the SL. Pretreatment with tempol or L-NAME was effective in protecting the noise-exposed animals from hearing loss, as well as in abolishing the noise-induced activation of the JNK signaling pathway. Interestingly, noise exposure caused a dramatic decrease in connexin26 level in the SL. This decrease was prevented by tempol or L-NAME. Taken together, our data suggest that noise-induced hearing loss is due at least in part to ROS / nitric oxide-mediated activation of the JNK pathway and down-regulation of connexin26 in the SL of mice.

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“…Upregulation of iNOS is particularly prominent in marginal cells of the stria vascularis and in strial vascular tissue following noise exposure (123,276,277), although some increase in expression also occurs in the organ of Corti. NO concentrations also increase in the lateral wall following noise exposure (51). In most studies, this upregulation is cited as evidence supporting a causative role for iNOS in producing the damage to the lateral wall that is a prominent feature of NIHL (111).…”

Section: Nos and Cochlear Pathologymentioning

confidence: 98%

Genetics of guanylyl cyclase pathways in the cochlea and their influence on hearing

Fitzakerley

Trachte

2018

Physiological Genomics

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Although hearing loss is the most common sensory deficit in Western societies, there are no successful pharmacological treatments for this disorder. Recent experiments have demonstrated that manipulation of intracellular cyclic guanosine monophosphate (cGMP) concentrations can have both beneficial and harmful effects on hearing. In this review, we will examine the role of cGMP as a key second messenger involved in many aspects of cochlear function and discuss the known functions of downstream effectors of cGMP in sound processing. The nitric oxide-stimulated soluble guanylyl cyclase system (sGC) and the two natriuretic peptide-stimulated particulate GCs (pGCs) will be more extensively covered because they have been studied most thoroughly. The cochlear GC systems are attractive targets for medical interventions that improve hearing while simultaneously representing an under investigated source of sensorineural hearing loss.

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Chronologic Changes of Nitric Oxide Concentration in the Cochlear Lateral Wall and Its Role in Noise‐Induced Permanent Threshold Shift

Cited by 8 publications

References 21 publications

The Effects of Noise on Oxidative and Antioxidative Balance in Human Erythrocytes

The Effects of Noise on Oxidative and Antioxidative Balance in Human Erythrocytes

Mechanism Underlying the Protective Effect of Tempol and Nω-Nitro-L-arginine Methyl Ester on Acoustic Injury: Possible Involvement of c-Jun N-Terminal Kinase Pathway and Connexin26 in the Cochlear Spiral Ligament

Genetics of guanylyl cyclase pathways in the cochlea and their influence on hearing

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