Chronic treatment with fluoride affects the jejunum: insights from proteomics and enteric innervation analysis

Dionízio, Aline; Melo, Carina Guimarães de Souza; Sabino-Arias, Isabela Tomazini; Ventura, Talita Mendes Oliveira; Leite, Aline de Lima; Souza, Sara Raquel Garcia de; Santos, Erika Xavier; Heubel, Alessandro Domingues; Souza, Juliana; Perles, Juliana Vanessa Colombo Martins; Zanoni, Jacqueline Nelisis; Buzalaf, Marília Afonso Rabelo

doi:10.1038/s41598-018-21533-4

Cited by 42 publications

(38 citation statements)

References 84 publications

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“…The mass spectrometry protocol was based on a previous study by Gabriel Kuniyoshi et al [ 84 ], Dionizio et al [ 85 ], and Da Silva-Gomes et al [ 86 ], with modifications. Briefly, protein extraction was carried out by homogenizing three VPs lobes from each experimental group on PND 21 and 540 in extraction buffer containing 0.01 M Tris-HCl, 0.005 M phenylmethylsulfonyl fluoride, 1% protease inhibitor, 0.065 M dithiothreitol, 8 M urea (in a proportion of 30mg tissue/100 μg buffer).…”

Section: Methodsmentioning

confidence: 99%

Identification of potential molecular pathways involved in prostate carcinogenesis in offspring exposed to maternal malnutrition

Santos

Camargo

Constantino

et al. 2020

aging

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The developmental origins of health and disease concept links adult diseases with early-life exposure to inappropriate environmental conditions. Intrauterine and postnatal malnutrition may lead to an increased incidence of type 2 diabetes, obesity, and cardiovascular diseases. Maternal malnutrition (MM) has also been associated with prostate carcinogenesis. However, the molecular mechanisms associated with this condition remain poorly understood. Using a proteomic analysis, we demonstrated that MM changed the levels of proteins associated with growth factors, estrogen signaling, detoxification, and energy metabolism in the prostate of both young and old rats. These animals also showed increased levels of molecular markers of endoplasmic reticulum function and histones. We further performed an in silico analysis that identified commonly deregulated proteins in the ventral prostate of old rats submitted to MM with a mouse model and patients with prostate cancer. In conclusion, our results demonstrated that estrogenic signaling pathways, endoplasmic reticulum functions, energy metabolism, and molecular sensors of protein folding and Ca2+ homeostasis, besides histone, and RAS-GTPase family appear to be involved in this process. Knowledge of these factors may raise discussions regarding the role of maternal dietary intervention as a public policy for the lifelong prevention of chronic diseases.

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Section: Methodsmentioning

confidence: 99%

Identification of potential molecular pathways involved in prostate carcinogenesis in offspring exposed to maternal malnutrition

Santos

Camargo

Constantino

et al. 2020

aging

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“…The current protocol was previously published by our group in [44,67,68]. First, the hippocampus from two animals were pooled and each group was carried out in biologic triplicate.…”

Section: Protein Extraction Digestion and Purificationmentioning

confidence: 99%

Hippocampal Impairment Triggered by Long-Term Lead Exposure from Adolescence to Adulthood in Rats: Insights from Molecular to Functional Levels

Oliveira

Dionízio

Teixeira

et al. 2020

IJMS

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Lead (Pb) is an environmental and occupational neurotoxicant after long-term exposure. This study aimed to investigate the effects of systemic Pb exposure in rats from adolescence to adulthood, evaluating molecular, morphologic and functional aspects of hippocampus. For this, male Wistar rats were exposed to 50 mg/kg of Pb acetate or distilled water for 55 days by intragastric gavage. For the evaluation of short-term and long-term memories, object recognition and step-down inhibitory avoidance tests were performed. At the end of the behavioral tests, the animals were euthanized and the hippocampus dissected and processed to the evaluation of: Pb content levels in hippocampal parenchyma; Trolox equivalent antioxidant capacity (TEAC), glutathione (GSH) and malondialdehyde (MDA) levels as parameters of oxidative stress and antioxidant status; global proteomic profile and neuronal degeneration by anti-NeuN immunohistochemistry analysis. Our results show the increase of Pb levels in the hippocampus of adult rats exposed from adolescence, increased MDA and GSH levels, modulation of proteins related to neural structure and physiology and reduced density of neurons, hence a poor cognitive performance on short and long-term memories. Then, the long-term exposure to Pb in this period of life may impair several biologic organizational levels of the hippocampal structure associated with functional damages.

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“…All proteomic analyses were performed according to protocols previously described by our group [71,72]. Firstly, the cerebellums from two animals were pooled and the analyses were carried out in triplicate.…”

Section: Proteomic Analysis: Preparation Of the Samplesmentioning

confidence: 99%

Long-Term Lead Exposure Since Adolescence Causes Proteomic and Morphological Alterations in the Cerebellum Associated with Motor Deficits in Adult Rats

Leão

Bittencourt

Oliveira

et al. 2020

IJMS

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Lead (Pb) is an environmental contaminant that presents a high risk for human health. We aimed to investigate the possible alterations triggered by the exposure to Pb acetate for a long period in motor performance and the possible relationship with biochemical, proteomic and morphological alterations in the cerebellum of rats. Male Wistar rats were exposed for 55 days, at 50 mg/Kg of Pb acetate, and the control animals received distilled water. Open field (OF) and rotarod tests; biochemistry parameters (MDA and nitrite); staining/immunostaining of Purkinje cells (PC), mature neurons (MN), myelin sheath (MS) and synaptic vesicles (SYN) and proteomic profile were analyzed. Pb deposition on the cerebellum area and this study drove to exploratory and locomotion deficits and a decrease in the number of PC, MN, SYN and MS staining/immunostaining. The levels of MDA and nitrite remained unchanged. The proteomic profile showed alterations in proteins responsible for neurotransmitters release, as well as receptor function and second messengers signaling, and also proteins involved in the process of apoptosis. Thus, we conclude that the long-term exposure to low Pb dose promoted locomotion and histological tracings, associated with alterations in the process of cell signaling, as well as death by apoptosis.

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Chronic treatment with fluoride affects the jejunum: insights from proteomics and enteric innervation analysis

Cited by 42 publications

References 84 publications

Identification of potential molecular pathways involved in prostate carcinogenesis in offspring exposed to maternal malnutrition

Identification of potential molecular pathways involved in prostate carcinogenesis in offspring exposed to maternal malnutrition

Hippocampal Impairment Triggered by Long-Term Lead Exposure from Adolescence to Adulthood in Rats: Insights from Molecular to Functional Levels

Long-Term Lead Exposure Since Adolescence Causes Proteomic and Morphological Alterations in the Cerebellum Associated with Motor Deficits in Adult Rats

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