Chronic toxicity of verapamil on juvenile rainbow trout (Oncorhynchus mykiss): Effects on morphological indices, hematological parameters and antioxidant responses

Li, Zhihua; Velíšek, Josef; Žlábek, Vladimír; Grabic, Roman; Máchová, Jana; Kolářová, Jitka; Li, Ping; Randák, Tomáš

doi:10.1016/j.jhazmat.2010.09.102

Cited by 123 publications

(56 citation statements)

References 65 publications

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“…Lower HSI have also been reported in O. mykiss, exposed to pharmaceutical drug verapamil [47], and in juvenile O. niloticus, exposed to paraquat [16]. Normal CF and HSI may indicate that paraquat does not affect the liver at the beginning of the exposure.…”

Section: Discussionmentioning

confidence: 90%

Physiological effects of paraquat in juvenile African catfish Clarias gariepinus (Burchel 1822)

2014

JCLM

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Section: Discussionmentioning

confidence: 90%

Physiological effects of paraquat in juvenile African catfish Clarias gariepinus (Burchel 1822)

2014

JCLM

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“…Even in small concentrations they can influence wildlife including fish. Li et al (2011) have shown multiple responses in fish (rainbow trout) indicating that verapamil induced physiological stress. To assess potential effects of verapamil on aquatic organisms the changes in male Siamese fighting fish (Betta splendens) behavior was studied, by testing aggressive behavior in this fish used as a model species.…”

Section: Introductionmentioning

confidence: 99%

Verapamil – L type voltage gated calcium channel inhibitor diminishes aggressive behavior in male Siamese fighting fish

Kania

Debski²,

Wrońska³

et al. 2015

Polish Journal of Veterinary Sciences

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Verapamil is a L-type voltage gated calcium channels inhibitor (VGCCI), which is a highly prescribed drug used in the treatment of hypertension, angina pectoris, cardiac arrhythmia and cluster headaches. Its common use caused its appearance in water environment. VGCC inhibit epinephrine release and cause many neuro-hormonal changes influencing also fish behavior. Siamese fighting fish was chosen to study the influence of verapamil given to the water on the beginning of experiment in 3 different concentrations of 0 (control), 8 and 160 μg · L -1 , on aggressive behavior in these fish. The experimental fish were placed in individual glass containers for 3 weeks and the mirror test was used. The highest concentration led to a significant modulation of fish behavior after 1 week and the lower dose caused statistically significant behavioral changes after 2 weeks of verapamil treatment. Siamese fighting fish males exposed to verapamil had longer latencies to the first chase -12.6 s (8 μg · L -1 of verapamil) and 18.8 s (160 μg · L -1 of verapamil) compared to 5.6 s in the control group, decreased attack frequency and shorter duration of these attacks. The number of attacks within 10 min was decreased from 38.3 in the control group to 27.1 and 16.1, respectively. Also the total duration of these attacks decreased from 354.8 (control) to 326.4 (decrease statistically insignificant) and to 194.8 s in verapamil treated groups. It was shown, that even relatively low concentrations of verapamil in water may have adverse effects on fish and probably other living organisms.

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“…Our results showed that treatment with sublethal concentrations of VRP significantly changed the physiological and biochemical responses (including behavior changes, morphological indices, hematological parameters, and antioxidant responses) (7,8). On the other hand, there was no significant change in all of the parameters measured in fish exposed to VRP at environmental related concentration (7).…”

mentioning

confidence: 54%

“…In this study, the abundance of GRP78 (glucoseregulated protein 78) and GRP94 (glucose-regulated protein 94) was significantly decreased in the livers of fish exposed to higher concentrations of VRP, which indicates the VRP-induced ER stress. Moreover, our previous results showed that long term exposure of VRP induces oxidative stress in fish liver and other tissues as well (7). Similarly, some previous studies reported that GRPs were involved in the tolerance response of oxidative stress, Ca 2ϩ disturbances, and cell death (23).…”

Section: Discussionmentioning

confidence: 75%

Hepatic Proteome Sensitivity in Rainbow Trout after Chronically Exposed to a Human Pharmaceutical Verapamil

Šulc

et al. 2012

Molecular & Cellular Proteomics

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Verapamil (VRP), a cardiovascular pharmaceutical widely distributed and persistent in the aquatic environment, has potential toxicity to fish and other aquatic organisms. However, the molecular mechanisms that lead to these toxic effects are not well known. In the present study, proteomic analysis has been performed to investigate the protein patterns that are differentially expressed in liver of rainbow trout exposed to sublethal concentrations of VRP (0.5, 27.0, and 270 g/liter) for 42 days. Two-dimensional electrophoresis coupled with MALDI-TOF/TOF mass spectrometry was employed to detect and identify the protein profiles. The analysis revealed that the expression of six hepatic acidic proteins were markedly altered in the treatment groups compared with the control group; three proteins especially were significantly down-regulated in fish exposed to VRP at environmental related concentration (0.5 g/liter). These results suggested that the VRP induce mechanisms against oxidative stress (glucoseregulated protein 78 and 94 and protein disulfide-isomerase A3) and adaptive changes in ion transference regulation (calreticulin, hyperosmotic glycine-rich protein).

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Chronic toxicity of verapamil on juvenile rainbow trout (Oncorhynchus mykiss): Effects on morphological indices, hematological parameters and antioxidant responses

Cited by 123 publications

References 65 publications

Physiological effects of paraquat in juvenile African catfish Clarias gariepinus (Burchel 1822)

Physiological effects of paraquat in juvenile African catfish Clarias gariepinus (Burchel 1822)

Verapamil – L type voltage gated calcium channel inhibitor diminishes aggressive behavior in male Siamese fighting fish

Hepatic Proteome Sensitivity in Rainbow Trout after Chronically Exposed to a Human Pharmaceutical Verapamil

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