Chronic toxicity of pesticides to the mRNA expression levels of metallothioneins and cytochrome P450 1A genes in rainbow trout

Abstract

The hazardous effects of pesticides on various metabolic pathways are a great problem for environmental health and should be well determined. In the present study, the authors treated rainbow trout with 0.6 μg/L deltamethrin for 28 days and 1.6 mg/L 2,2-dichlorovinyl dimethyl phosphate for 21 days. After this time period, the authors observed alterations in mRNA expression levels of MT-A, MT-B and CYP-1A. Chronic exposure to low levels of pesticides may have a more significant effect on fish populations than acute poisoning. While both pesticides caused a significant increase on mRNA levels of MT-A and CYP-1A, MT-B mRNA levels were increased significantly only upon deltamethin administration. The significant increase in mRNA levels of the corresponding genes may be considered as a defence mechanism in addition to the antioxidants against oxidative stress, as well as a detoxification mechanism against adverse effects of pesticides.

Effects of glyphosate on juvenile rainbow trout (Oncorhynchus mykiss): Transcriptional and enzymatic analyses of antioxidant defence system, histopathological liver damage and swimming performance

“…In order to perform multiplex Real Time PCR, TaqMan probe of the housekeeping gene was conjugated with Cy5/Blackhole Quencher 2, the fluorophore and quencher molecules, whereas the probes for target genes were conjugated with FAM/ TAMRA (Ceyhun et al, 2011). The primer and probe sequences are provided in Table 1.…”

Combined effects of DDE and hyperlipidic diet on metallothionein expression and synthesis in rat tissues

“…Thanks to their multiple properties, MTs exert a protective role against obesity and high fat‐induced effects, such as insulin resistance, impairment of mitochondrial function and oxidative stress . In addition, MTs attenuate pesticides (such as paraquat and deltamethrin) injury through mechanisms blocking apoptosis and oxidation; a pesticides‐induction in MT expression has been also observed . The aim of this work was to verify the involvement of MT in cellular response to DDE contamination in vivo, using rat tissues as model study.…”