Chronic Strongyloides stercoralis infection increases presence of the Ruminococcus torques group in the gut and alters the microbial proteome

Abstract

We explored the impact of chronic Strongyloides stercoralis infection on the gut microbiome and microbial activity in a longitudinal study. At baseline (time-point T0), 42 fecal samples from matched individuals (21 positive for strongyloidiasis and 21 negative) were subjected to microbiome 16S-rRNA sequencing. Those positive at T0 (untreated then because of COVID19 lockdowns) were retested one year later (T1). Persistent infection in these individuals indicated chronic strongyloidiasis: they were treated with ivermectin and retested four months later (T2). Fecal samples at T1 and T2 were subjected to 16S-rRNA sequencing and LC–MS/MS to determine microbial diversity and proteomes. No significant alteration of indices of gut microbial diversity was found in chronic strongyloidiasis. However, the Ruminococcus torques group was highly over-represented in chronic infection. Metaproteome data revealed enrichment of Ruminococcus torques mucin-degrader enzymes in infection, possibly influencing the ability of the host to expel parasites. Metaproteomics indicated an increase in carbohydrate metabolism and Bacteroidaceae accounted for this change in chronic infection. STITCH interaction networks explored highly expressed microbial proteins before treatment and short-chain fatty acids involved in the synthesis of acetate. In conclusion, our data indicate that chronic S. stercoralis infection increases Ruminococcus torques group and alters the microbial proteome.

Promising probiotic-fermented soymilk for alleviating acute diarrhea: insights into the microbiome and metabolomics

“…27 Anaerofilum is markedly associated with inflammatory agents related to increased lipid metabolic disorders. 28 However, previous findings revealed a potential beneficial role of Anaerostipes and Fusicatenibacter, 29 but our research demonstrated the opposite results. These bacteria cited above are closely related to pro-inflammatory cytokines and specific lipid metabolisms, which can promote the expression of serum inflammation in rats.…”

Strongyloides stercoralis infection reduces Fusicatenibacter and Anaerostipes in the gut and increases bacterial amino-acid metabolism in early-stage chronic kidney disease

“…A recent study from the same region of Thailand also found no significant differences in gut microbiome structure and diversity between healthy individuals with or without S. stercoralis infection [ 23 ]. Recently, a longitudinal study found no significant changes in gut diversity before and after treatment of S. stercoralis infection in healthy individuals [ 37 ].…”

Role of N-acetylkynurenine in mediating the effect of gut microbiota on urinary tract infection: a Mendelian randomization study

“…Consistent with prior studies ( Worby et al, 2022b ), the abundance of Ruminococcaceae decreased in patients with recurrent UTI, suggesting that Ruminococcus could serve as a potential marker for dysbiosis in recurrent cystitis ( Graziani et al, 2022 ). The f_Ruminococcaceae is also known to be significantly affected by chronic infections ( Martinez et al, 2022 ; Tran et al, 2023 ) and the administration of antibiotics ( Ross et al, 2016 ). Although direct evidence linking Ruminococcaceae UCG010 to UTI remains elusive, some studies have proposed that a higher abundance of this bacterium in the GM may correlate with decreased risks of type 2 diabetes ( Lyu et al, 2023 ), non-alcoholic fatty liver disease ( Tsai et al, 2020 ), and obesity ( Nseir et al, 2015 ), each being a risk factor for UTI.…”