Chronic Stress Effects on Adrenal Cortex Cellular Proliferation in Pregnant Rats

Bozzo, Aída Andrea; Soñez, Carlos Alberto; Cobeta, Ignacio; Ávila, Rodolfo Esteban; Rolando, Alicia Nélida; Romanini, María Cristina; Lazarte, Mario; Gauna, Héctor Fernando; Mugnaini, María Teresa

doi:10.4067/s0717-95022011000400013

Cited by 7 publications

(6 citation statements)

References 22 publications

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“…Ultrastructuraly, the cells showed electron-dense cytoplasm and the mitochondria showed disrupted cristae. These changes have been considered as signs of lowered functional activity of steroid-producing adrenal cells [27] and correlate well with adrenocortical overstimulation and /or degeneration [28,29] . Many circulatory disorders were expected as a result of compression of the hypertrophied cells on the sinusoidal capillaries.…”

Section: Discussionmentioning

confidence: 99%

Histological study of the effect of tributyltin on the adrenal cortical cells of adult male albino rats.

Solaiman

seddik

2019

Egyptian Journal of Histology

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Background:Tributyltin is widely used as antifouling paint for marine vessels. It is slowly released from the paint to the water and becomes incorporated into soil, animals and plants. TBT has been identified as an endocrine disruptor and induces oxidative damage. Objectives: This study aimed to investigate the probable toxic effect of tributyltin on adrenal cortical cells of adult male albino rats for two different durations. Materials and Methods: Twenty four adult male albino rats were divided into 3 equal groups: Group I: was the control group; Group II: each rat received tributyltin orally at a dose of 1mg daily for one week. Group III: received the same daily dose for successive two weeks. At the end of the experiment, the animals were sacrificed and blood samples were subjected to hormonal assay for aldosterone, corticosterone and ACTH levels. Serum tin levels were assessed. The adrenal tissue was processed for light and electron microscopic examination. In addition, immunoreaction for CD 44 positive cells was performed. Results: A significant decrease in aldosterone and corticosterone, an increase in ACTH levels and an increase in the mean count of CD44 positive cells in group III have been revealed when compared with the other groups. Histological changes in group II were shown in zona glomerulosa and fasciculata in the form of loss of architecture, cytoplasmic vacuolation and disrupted mitochondrial cristae. Meanwhile, Group III showed few lipid droplets and deeply stained nuclei in both zones. Conclusion:Tributyltin has deleterious effects on cells of zona glomerulosa and zona fasciculata of the adrenal cortex of adult male albino rats which were time-dependent.

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Section: Discussionmentioning

confidence: 99%

Histological study of the effect of tributyltin on the adrenal cortical cells of adult male albino rats.

Solaiman

seddik

2019

Egyptian Journal of Histology

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“…One of the distinctive findings observed in this study is the allocation of the proliferative cells at the junction of ZG and ZF and in the outer ZF. This finding may be explained according to one of the theories mentioned by (Bozzo et al, 2011) who reported that the cellular replacement in different adrenal cortical zones could be assigned to the transformation theory which proposed that the cells proliferate in an intermediate zone between ZG and ZF then the cells migrate towards both the capsule and the medulla.…”

Section: Advances In Animal Andmentioning

confidence: 81%

Effect of Experimentally Induced Depression on the Adrenal Cortex of Adult Male Albino Rats and the Possible Ameliorative Role of Voluntary Exercise

Eldomiaty¹,

Elsawaf²,

Ali³

et al. 2023

AAVS

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This study was designed to investigate the changes that occur in adrenal cortex in an animal model of depression and the effect of voluntary exercise on these changes. Thirty-two adults male Wistar rats were included based on their forced swimming test behavior. The rats were divided into 4 groups. Group 1 was the control group, group II was the control exercise group, group III was the depression group, and group IV was the depression exercise group. The forced swimming protocol was used to induce depression, while the rat voluntary wheel was used for voluntary exercise. After scarification, estimation of corticosterone level was conducted, and samples of adrenal gland were examined for structural changes by light and Electron microscope, and for immunohistochemical expression. Rats from group III showed statistically increased corticosterone level and increased cortical thickness compared to other groups. Voluntary exercise improved these measures in rats of group IV. Histological disorganization of cortical cells was observed in group III with manifest large cytoplasmic vacuoles. Enormous increase in the number of both apoptotic and proliferative cells was seen with significantly higher mean number of the proliferative cells. By EM, the nuclei were shrunken, secretory vesicles were depleted, and the smooth endoplasmic reticulum showed massive dilatation. These findings were partially restored in rats of group IV. This study concluded that voluntary exercise enhanced recovery from the injurious effect of depression on adrenal cortical cells that partially showed regain of their normal structure and corticosterone secretion.

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“…We also found that chronic stress in pregnant rats caused a reduction in proliferation (Bozzo et al 2011) and apoptosis in the adrenal cortex. Our results indicate a lower degree turnover of adrenal cortical cells.…”

Section: Chronic Stress and Adrenal Apoptosis 301mentioning

confidence: 87%

“…Earlier we demonstrated that the cell proliferation index (CPI) of the reticular zone in both control and experimental rats was signifi cantly decreased compared to the glomerular and fascicular zones for all three stages of gestation studied (Bozzo et al 2011).…”

Section: Declaration Of Interestmentioning

confidence: 98%

Chronic stress and its effects on adrenal cortex apoptosis in pregnant rats

Bozzo

Soñez

Cobeta

et al. 2013

Biotechnic & Histochemistry

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The model of chronic intermittent stress by immobilization during pregnancy may produce alterations in the mechanisms that maintain adrenal gland homeostasis. In earlier investigations using this model, significant variations in plasma prolactin and corticosterone levels, and adrenal gland weights were observed. We hypothesized that chronic stress causes changes in apoptosis in the adrenal glands of pregnant rats. We identified and quantified apoptotic cells in the adrenal cortex and examined their ultrastructural characteristics using transmission electron microscopy. Adrenal glands of pregnant rats at gestation days 12, 17 and 21 were studied for control and experimental (stressed) rats. Immunolabelling techniques, stereological analysis and image quantification of adrenal gland sections were combined to determine differences in apoptosis in the different cell populations of the adrenal cortex. The apoptotic index of the experimental rats showed a significant reduction at gestation day 17, while at days 12 and 21 there were no differences from controls. Moreover, the apoptotic index of the reticular zones in control and experimental animals showed a significant increase compared to the glomerular and fascicular zones at the three gestation times studied. Chronic stress by immobilization reduced the caspase-dependent apoptotic index at gestation day 17, which may be related to variations in plasma concentrations of estrogens and prolactin.

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Chronic Stress Effects on Adrenal Cortex Cellular Proliferation in Pregnant Rats

Cited by 7 publications

References 22 publications

Histological study of the effect of tributyltin on the adrenal cortical cells of adult male albino rats.

Histological study of the effect of tributyltin on the adrenal cortical cells of adult male albino rats.

Effect of Experimentally Induced Depression on the Adrenal Cortex of Adult Male Albino Rats and the Possible Ameliorative Role of Voluntary Exercise

Chronic stress and its effects on adrenal cortex apoptosis in pregnant rats

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