Chronic schisandrin B treatment improves mitochondrial antioxidant status and tissue heat shock protein production in various tissues of young adult and middle-aged rats

Abstract

The effects of chronic schisandrin B (Sch B) treatment (10 mg/kg/dayx15) on mitochondrial antioxidant status and sensitivity to Ca2+-induced permeability transition, as well as tissue heat shock protein (Hsp)25/70 production were examined in various tissues (brain, heart, liver, skeletal muscle) of young adult and middle-aged female rats. Age-dependent impairment in mitochondrial antioxidant status, as assessed by levels/activities of antioxidant components (reduced glutathione, alpha-tocopherol, Se-glutathione peroxidase and Mn-superoxide dismutase) and the extent of reactive oxygen species generation in vitro, was observed in brain, heart, liver and skeletal muscle tissues. While tissue Hsp25 levels remained relatively unchanged with aging, the Hsp70 level was increased in both brain and heart tissues of middle-aged rats. Chronic Sch B treatment was able to enhance mitochondrial antioxidant status and the resistance to Ca2+-induced mitochondrial permeability transition in an age-independent manner in various tissues of rats. However, Hsp25 and Hsp70 levels were only increased in young adult rats. The Sch B-induced enhancement of mitochondrial protective parameters in the heart was associated with the protection against myocardial ischemia-reperfusion injury in both young adult and middle-aged rats. The results suggest that chronic Sch B treatment may be beneficial for reversing the mitochondrial changes with aging and enhancing the heat shock response.

Schisandrin B Enhances Cerebral Mitochondrial Antioxidant Status and Structural Integrity, and Protects against Cerebral Ischemia/Reperfusion Injury in Rats

“…It has been shown that the same Sch B treatment regimen also protected against myocardial I/R injury in both young and middle-aged rats. 2) In this regard, we also found that long-term Sch B treatment at a daily dose of 30 mg/kg for 15 d protected against cerebral I/R injury in 2-year old rats, with the degree of protection being larger than that of the young rats (unpublished data). Sch B may therefore be generally used as hormetic agent for preventing myocardial and cerebral I/R injury occurring at both young and old ages.…”

“…2) Given that the maintenance of mitochondrial antioxidant status and structural integrity is crucial for cell survival, 3) Sch B has been proposed to be used as a universal cell protectant against tissue damage caused by endogenous and exogenous oxidants. 2) While the protection of Sch B against I/R injury has been demonstrated in the heart, 2,4) it is still unclear whether Sch B treatment can produce any beneficial effect on cerebral I/R injury.…”

Schisandrin B Prevents Doxorubicin Induced Cardiac Dysfunction by Modulation of DNA Damage, Oxidative Stress and Inflammation Through Inhibition of MAPK/p53 Signaling

“…Recently, we identified the neuroprotective property of Sch B in scopolamine induced dementia. Previous studies have demonstrated that Sch B protected against myocardial ischemia/ reperfusion (I/R) injury and also possesses strong anti-inflammatory property in in-vivo and invitro model [9,15]. In addition, we have showed that Sch B specifically inhibits phosphorylation of p53 through ATR inhibition in in-vitro model [16].…”

“…It is also a major component herb of many traditional herbal medicines including SMS. Cardiac and neuroprotective function of FS and the lignans of FS have also been published [9,10,14]. Previous studies have demonstrated that Sch B a major lignan of FS protected against myocardial ischemia/ reperfusion (I/R) injury and also possesses strong anti-inflammatory property in in-vivo and in-vitro model [9,15].…”

Schisandrin B Enhances Hepatic/Myocardial Glutathione Regeneration Capacity and Protects Against Oxidant Injury in Rat Livers and Hearts

“…Previous studies have shown that treatment with Sch B (0.825 μmol/kg/day) for 15 consecutive days induced a glutathione antioxidant response and confer a protective effect in rat hearts [10]. In the study, rats were intragastrically administered Sch B, curcumin, EGCG or resveratrol (0.825 μmol/kg/day, 0.5 mL per rat) for 15 consecutive days.…”