Chronic relapsing experimental allergic encephalomyelitis: Identification and dynamics of T and B cells within the central nervous system

Inflammatory cells, microglia and MHC class II antigen-positive cells in the spinal cord of Lewis rats with acute and chronic relapsing experimental autoimmune encephalomyelitis

“…Polman et al (1988) found T cells in the CNS in this model of CR-EAE 50 days after inoculation, but did not study rats throughout the course of CR-EAE. Immunocytochemical studies of CR-EAE in the guinea pig have demonstrated T cells in the spinal cord (Traugott et al, 1982;Antoniou et al, 1987a;Butter et al, 1989). In mice, immunocytochemical studies demonstrated CD4 + T cells in the CNS in relapses of CR-EAE (Traugott et al, 1986;Baker et al, 1990) and radio-labelling has demonstrated passively transferred cells in the nervous system, especially in perivascular cuffs, in CR-EAE in SJL/J mice (Cross et al, 1990).…”

“…In a previous morphological study we found that plasma cells were prominent in the spinal cord in the later stages of this form of CR-EAE . Immunocytochemical studies have demonstrated many plasma cells and B cells in the CNS of guinea pigs with CR-EAE (Grundke-Igbal et al, 1980;Traugott et al, 1982;Bernheimer et al, 1988). As B cells can differentiate into plasma cells in target organs (Lee et al, 1992), antibody production in the CNS may contribute to the pathogenesis of CR-EAE, although circulating antibody also has a role (Linington and Lassmann, 1987;Linington et al, 1988;Gautam et al, 1991).…”

“…In the guinea pig (Traugott et al, 1982;Butter et al, 1989) and mouse (Traugott et al, 1986;Baker et al, 1990) models of CR-EAE, immunocytochemical studies have demonstrated T cells and macrophages in the central nervous system (CNS). In CR-EAE induced in the Lewis rat by treatment with low dose CsA, Polman et al (1988) found T cells and macrophages in the CNS 50 days after inoculation.…”

RORγt-specific transcriptional interactomic inhibition suppresses autoimmunity associated with T H 17 cells

“…T cells are known to be crucial for inducing EAE, animal model of MS, where the inflammatory lesions are characterized by massive infiltration of inflammatory cells, inducing T cells, B cells, and macrophages (27,28). Previously, it has been agreed in the field that only T H 1 plays a critical role in neurologic inflammatory disease, but recent reports have emphasized the pathogenic role of T H 17 cells and T cells secreting IL-17/IFN-γ together rather than that of T H 1 (29).…”

Characterization and distribution of lymphocyte subpopulations in multiple sclerosis plaques versus autoimmune demyelinating lesions

“…Strain 13 guinea pigs, inoculated for acute EAE with isogenic spinal cord in CFA, develop clinical signs between 12 and 20 days post-inoculation (PI) and succumb within the following 2 days. In our initial studies on CNS tissue from Strain 13 guinea pigs with acute EAE, pan T cells were labeled with a mouse monoclonal antibody against guinea pig peripheral T cells in combination with FITC-labeled rabbit anti-mouse Ig [79,81,82]. B cells and Ig-containing macrophages, C 3 complement component, and albumin were localized by direct fluorescence using rhodamine-conjugated rabbit anti-guinea pig IgG and other appropriate FITC or rhodamine-conjugated polyclonal antibodies.…”