Chronic pulmonary accumulation of iron oxide nanoparticles induced Th1-type immune response stimulating the function of antigen-presenting cells

Park, Eun-Jung; Oh, Seung Yun; Lee, Sang Jin; Lee, Kyuhong; Kim, Younghun; Lee, Byoung‐Seok; Kim, Jong Sung

doi:10.1016/j.envres.2015.09.030

Cited by 53 publications

(51 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As reported previously, splenocytes were obtained by grounding the spleen (8 mice per group) in RPMI media containing fetal bovine serum (2%) and washed once with PBS (Park et al ., ). After removing red cells with FACS lysis buffer, splenocytes were filtered using a 70 μm‐pore size nylon mesh, and then were resuspended in FACS buffer.…”

Section: Methodsmentioning

confidence: 97%

“…Bronchoalveolar lavage (BAL) fluids (1400–1700 μl) were obtained by cannulating the trachea and lavaging the lungs twice with cold sterile PBS (1 ml) on day 90 after a single instillation. After centrifuging at 1500 rpm (231 g) for 5 min, cell pellets were used for BAL cell (total cell counts and differentiation) and cell‐cycle analysis, and the supernatants were used to measure the cytokine level (Park et al ., ; Park et al ., ). BAL cell analysis was performed according to the standard operating procedure of Korea Institute of Toxicology, a good laboratory practice institute in Korea.…”

Section: Methodsmentioning

confidence: 98%

See 1 more Smart Citation

Pulmonary persistence of graphene nanoplatelets may disturb physiological and immunological homeostasis

Park

Lee

et al. 2016

J. Appl. Toxicol.

Self Cite

View full text Add to dashboard Cite

Accumulated evidence suggests that chronic pulmonary accumulation of harmful particles cause adverse pulmonary and systemic health effects. In our previous study, most of the graphene nanoplatelet (GNP) remained in the lung until 28 days after a single instillation. In this study, we sought to evaluate the local and systemic health effect after a long pulmonary persistence of GNP. As expected, GNP remained in the lung on day 90 after a single intratracheal instillation (1.25, 2.5 and 5 mg kg ). In the lung exposed at the highest dose, the total number of cells and the percentage of lymphocytes significantly increased in the BAL fluid with an increase in both the number of GNP-engulfed macrophages and the percentage of apoptotic cells. A Th1-shifted immune response, the elevated chemokine secretion and the enhanced expression of cytoskeletal-related genes were observed. Additionally, the expression of natriuretic-related genes was noteworthy altered in the lungs. Moreover, the number of white blood cells (WBC) and the percentage of macrophages and neutrophils clearly increased in the blood of mice exposed to a 5-mg kg dose, whereas total protein, BUN and potassium levels significantly decreased. In conclusion, we suggest that the long persistence of GNP in the lung may cause adverse health effects by disturbing immunological- and physiological-homeostasis of our body. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 98%

Pulmonary persistence of graphene nanoplatelets may disturb physiological and immunological homeostasis

Park

Lee

et al. 2016

J. Appl. Toxicol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The concentrations of pro‐inflammatory cytokines [interleukin (IL)−1β, tumor necrosis factor (TNF)‐α, and IL‐6], Th0‐type cytokine (IL‐2), Th1‐type cytokine (IL‐12/IL‐23), Th2‐type cytokines (IL‐4, IL‐5, IL‐10, and IL‐13), chemokines [macrophage inflammatory protein (MIP)−1α, macrophage chemoattractant protein (MCP)−1, and granulocyte‐macrophage colony‐stimulating factor (GM‐CSF)], and transforming growth factor (TGF)‐β were determined by enzyme‐linked immunosorbent assay (ELISA) according to the manufacturer's instructions (eBioscience). The reactions were stopped by adding 1 M H 3 PO 4 solution, and the absorbance was measured at 450 nm using an ELISA reader (Molecular Devices).…”

Section: Methodsmentioning

confidence: 99%

“…As previously reported, MH‐S cells (20 μg/mL) and lung tissues (100 μg/mouse) were fixed in 2% glutaraldehyde/0.1 M sodium cacodylate buffer (pH 7.2) for 2 h …”

Section: Methodsmentioning

confidence: 99%

“…As previously reported, MH-S cells (20 lg/mL) and lung tissues (100 lg/mouse) were fixed in 2% glutaraldehyde/0.1 M sodium cacodylate buffer (pH 7.2) for 2 h. 15,19 Then, the samples were stained for 30 min with 0.5% aqueous uranyl acetate, dehydrated in graded ethanol solutions, and embedded in Spurr's resin. Thin sections were cut using an ultramicrotome (MT-X, RMC, Tucson, AZ, USA), stained with 2% uranyl acetate and Reynolds's lead citrate, and imaged with a LIBRA 120 TEM (Zeiss, Oberkochen, Germany) at an accelerating voltage of 80 kV.…”

Section: Tem Imagementioning

confidence: 99%

See 1 more Smart Citation

Pulmonary glass particles may persist in the lung suppressing function of immune cells

Park

Lee

Kim

et al. 2017

Environmental Toxicology

Self Cite

View full text Add to dashboard Cite

The health effects of silica may depend on the inherent properties of crystalline silica or on external factors affecting the biological activity or distribution of its polymorphs. Inhaled crystalline silica is classified as a Group I carcinogen, however, information on the health effects of amorphous silica is still insufficient. Considering that alveolar macrophages play a key role in both innate and adaptive immune responses for removal of foreign bodies that enter via the respiratory system, we treated sheet-like glass particles (SGPs), a type of noncrystalline amorphous silica, to MH-S cells, an alveolar macrophage cell line. SGPs reduced the generation of ROS and NO and induced cell death via multiple pathways. Although the expression of CD80, CD86, and CD40, increased by exposure to SGPs, the expression of MHC class II molecules had not notably changed. Additionally, expression of ICAM-1 tended to decrease. In mice, SGPs were distributed in the interstitial region of the lung without notable pathological lesion on day 14 after a single intratracheal instillation. Pulmonary total cell number increased significantly with the highest dose, but the levels of all measured inflammatory cytokines and chemokines, except IL-1, were lower in BAL fluid from SGP-treated mice compared to control. More interestingly, the expression of antigen presentation-related proteins was enhanced in the lungs of SGP-exposed mice concomitant with an increase in the number of mature dendritic cells, whereas the expression of ICAM-1, an important adhesion molecule for helper T cell recruitment, was suppressed. Taken together, we suggest that SGPs may induce adverse health effects by down-regulating function of immune cells in the lungs. Furthermore, ICAM-1 may play a key role in immune response to remove pulmonary SGPs.

show abstract

Immunotoxicity Considerations for Next Generation Cancer Nanomedicines

et al. 2019

View full text Add to dashboard Cite

Although interest and funding in nanotechnology for oncological applications is thriving, translating these novel therapeutics through the earliest stages of preclinical assessment remains challenging. Upon intravenous administration, nanomaterials interact with constituents of the blood inducing a wide range of associated immunotoxic effects. The literature on the immunological interactions of nanomaterials is vast and complicated. A small change in a particular characteristic of a nanomaterial (e.g., size, shape, or charge) can have a significant effect on its immunological profile in vivo, and poor selection of specific assays for establishing these undesirable effects can overlook this issue until the latest stages of preclinical assessment. This work describes the current literature on unintentional immunological effects associated with promising cancer nanomaterials (liposomes, dendrimers, mesoporous silica, iron oxide, gold, and quantum dots) and puts focus on what is missing in current preclinical evaluations. Opportunities for avoiding or limiting immunotoxicity through efficient preclinical assessment are discussed, with an emphasis placed on current regulatory views and requirements. Careful consideration of these issues will ensure a more efficient preclinical assessment of cancer nanomedicines, enabling a smoother clinical translation with less failures in the future.

show abstract

Chronic pulmonary accumulation of iron oxide nanoparticles induced Th1-type immune response stimulating the function of antigen-presenting cells

Cited by 53 publications

References 46 publications

Pulmonary persistence of graphene nanoplatelets may disturb physiological and immunological homeostasis

Pulmonary persistence of graphene nanoplatelets may disturb physiological and immunological homeostasis

Pulmonary glass particles may persist in the lung suppressing function of immune cells

Immunotoxicity Considerations for Next Generation Cancer Nanomedicines

Contact Info

Product

Resources

About