Chronic Pain Causes Peripheral and Central Responses in MIA-Induced TMJOA Rats

Jiang, Huijuan; Xu, Liqin; Li, Wen; Xiao, Meixia; Jin, Ke; Long, Xing

doi:10.1007/s10571-020-01033-8

Cited by 21 publications

(23 citation statements)

References 45 publications

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“…Due to the narrow space of the TMJ cavity in mice, great difficulty has been issued on the direct puncture of the TMJ cavity [ 41 ]. Here, we utilized the zygomatic process approached anterosuperior puncture technique (ASPT), which has been reported to have high success rates in previous studies [ 41 , 42 , 43 ]. The successful transplantation of GFP-labeled BMSCs into the TMJ region were confirmed by immunostaining.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Circadian Clock in MSCs-Based Cytotherapy Ameliorates Age-Related Temporomandibular Joint Condyle Degeneration

Cha

Lee

Wang

et al. 2021

IJMS

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Aging has been proven to be one of the major causes of temporomandibular joint (TMJ) disability and pain in older people. Peripheral circadian rhythms play a crucial role in endochondral ossification and chondrogenesis. However, the age-related alterations of circadian clock in TMJ structures are seldom reported. In the current study, TMJ condyles were extracted from young (4-month-old), middle-aged (10-month-old), and old-aged (20-month-old) adults to detect the morphology and circadian oscillation changes in TMJ condyles with aging. The transcriptome profile of Bmal1-deleted bone-marrow mesenchymal stem cells (BMSCs) and controls were explored to reveal the circadian-related differences at the molecular level. Furthermore, the reparative effects of Bmal1-overexpressed BMSCs-based cytotherapy in aged TMJ condyles were investigated in vitro and in vivo. Aged TMJ condyles displayed damaged tissue structure and an abolished circadian rhythm, accompanied by a progressively decreasing chondrogenesis capability and bone turnover activities. The deletion of Bmal1 significantly down-regulated chondrogenesis-related genes Prg4, Sox9, and Col7a1. Bmal1-overexpressed BMSCs presented improved migration capability ex vivo and attenuated age-related TMJ condylar degeneration in vivo. These data demonstrate the crucial role of circadian timing in the maintenance of osteochondral homeostasis, and indicate the potential clinical prospects of circadian-modified MSCs therapy in tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Enhanced Circadian Clock in MSCs-Based Cytotherapy Ameliorates Age-Related Temporomandibular Joint Condyle Degeneration

Cha

Lee

Wang

et al. 2021

IJMS

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“…The experimental model of TMJOA pain was established per our previous study (Jiang et al 2021). Briefly, MIA (50 μL, 1.0 mg/50 μL) or saline (50 μL) was bilaterally and slowly injected into the upper compartment of the TMJ in rats under isoflurane anesthesia.…”

Section: Rat Models Of Tmjoa Chronic Painmentioning

confidence: 99%

“…Briefly, MIA (50 μL, 1.0 mg/50 μL) or saline (50 μL) was bilaterally and slowly injected into the upper compartment of the TMJ in rats under isoflurane anesthesia. Our previous studies showed that chronic pain in TMJOA rats induced by MIA injection started at day 14 (Jiang et al 2021). To minimize the suffering of the animals, day 14 was therefore selected as the time point for the following studies evaluating the analgesic profile of MT (Fig.…”

Section: Rat Models Of Tmjoa Chronic Painmentioning

confidence: 99%

“…Chronic pain is the main cause for patients with temporomandibular joint osteoarthritis (TMJOA) to seek medical care (Jiang et al 2021), and it is a major challenge for clinicians (Kuner 2010). Hence, relieving pain has become the primary task in the treatment of TMJOA.…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, large myelinated A-fiber TG neurons are marked with neurofilament 200 (NF200; Chen et al 2017; Zhang et al 2018). We recently showed that CGRP + and IB4 + neurons rather than NF200 + neurons in TG were sensitized in a monosodium iodoacetate (MIA)–induced TMJOA chronic pain rat model (Jiang et al 2021). However, the expression of MTR in these neurons is still unknown, and whether MTR influences the sensitization of CGRP + and IB4 + TG neurons induced by TMJOA needs to be explored.…”

Section: Introductionmentioning

confidence: 99%

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Melatonin Abates TMJOA Chronic Pain by MT₂R in Trigeminal Ganglion Neurons

et al. 2021

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Temporomandibular joint osteoarthritis (TMJOA) is one of the most common diseases causing chronic pain in the oral and maxillofacial region. So far, there are few ways to relieve the pain of TMJOA. Melatonin (MT) has a good analgesic effect in many diseases, including fibromyalgia, neuropathic pain, chronic headache, and burn pain, with very low acute toxicity and side effects. This study was to investigate the role and mechanism of MT in TMJOA chronic pain. In rats TMJOA chronic pain occurred at day 14 after an intra–temporomandibular joint injection of monosodium iodoacetate, which we previously reported. The enzyme-linked immunosorbent assay results showed that MT levels were higher in the synovial fluid from patients and rats with TMJOA as compared with those from control. Fluorescent retrograde tracing (Dil) identified that upregulation of MT type 2 receptor (MT2R) in trigeminal ganglion (TG) neurons innervating rat temporomandibular joints was accompanied by TMJOA chronic pain. Nociceptive behavior as assessed by von Frey and the Rat Grimace Scale demonstrated that exogenous administration of MT relieved chronic pain in TMJOA rats, whereas blocking MT2R with 4P-PDOT reversed the analgesic effect of MT. Immunofluorescence analysis also confirmed that MT inhibited CGRP and IB4 expression of TG neurons, and this inhibition was reversed by administering the MT2R antagonist in TMJOA rats. By using Fluo-3 AM-based calcium imaging in vitro, MT elicited calcium transients in Dil+ TG neurons, which were significantly abolished by 4P-PDOT. Collectively, this study suggested that MT relieves the TMJOA chronic pain of rats through downregulation of sensitized CGRP+ and IB4+ neurons in TG via MT2R. This will be helpful for health care professionals utilizing MT as an option against TMJOA chronic pain.

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Expression of CGRP in the Trigeminal Ganglion and Its Effect on the Polarization of Macrophages in Rats with Temporomandibular Arthritis

Tao,

Wang,

2024

Cell Mol Neurobiol

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Calcitonin gene-related peptide (CGRP) is synthesized and secreted by trigeminal ganglion neurons, and is a key neuropeptide involved in pain and immune regulation. This study investigates the expression of CGRP in the trigeminal ganglion (TG) and its regulatory role in the polarization of macrophages in rats with temporomandibular arthritis. A rat model of temporomandibular arthritis was established using CFA. Pain behavior was then observed. Temporomandibular joint (TMJ) and the TG were collected, and immunohistochemistry, immunofluorescence (IF) staining, and RT-qPCR were used to examine the expression of CGRP and macrophage-related factors. To investigate the impact of CGRP on macrophage polarization, both CGRP and its antagonist, CGRP 8-37, were separately administered directly within the TG. Statistical analysis revealed that within 24 h of inducing temporomandibular arthritis using CFA, there was a significant surge in CD86 positive macrophages within the ganglion. These macrophages peaked on the 7th day before beginning their decline. In this context, it’s noteworthy that administering CGRP to the trigeminal ganglion can prompt these macrophages to adopt the M2 phenotype. Intriguingly, this study demonstrates that injecting the CGRP receptor antagonist (CGRP 8-37) to the ganglion counteracts this shift towards the M2 phenotype. Supporting these in vivo observations, we found that in vitro, CGRP indeed fosters the M2-type polarization of macrophages. CGRP can facilitate the conversion of macrophages into the M2 phenotype. The phenotypic alterations of macrophages within the TG could be instrumental in initiating and further driving the progression of TMJ disorders.

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Chronic Pain Causes Peripheral and Central Responses in MIA-Induced TMJOA Rats

Cited by 21 publications

References 45 publications

Enhanced Circadian Clock in MSCs-Based Cytotherapy Ameliorates Age-Related Temporomandibular Joint Condyle Degeneration

Enhanced Circadian Clock in MSCs-Based Cytotherapy Ameliorates Age-Related Temporomandibular Joint Condyle Degeneration

Melatonin Abates TMJOA Chronic Pain by MT₂R in Trigeminal Ganglion Neurons

Expression of CGRP in the Trigeminal Ganglion and Its Effect on the Polarization of Macrophages in Rats with Temporomandibular Arthritis

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Chronic Pain Causes Peripheral and Central Responses in MIA-Induced TMJOA Rats

Cited by 21 publications

References 45 publications

Enhanced Circadian Clock in MSCs-Based Cytotherapy Ameliorates Age-Related Temporomandibular Joint Condyle Degeneration

Enhanced Circadian Clock in MSCs-Based Cytotherapy Ameliorates Age-Related Temporomandibular Joint Condyle Degeneration

Melatonin Abates TMJOA Chronic Pain by MT2R in Trigeminal Ganglion Neurons

Expression of CGRP in the Trigeminal Ganglion and Its Effect on the Polarization of Macrophages in Rats with Temporomandibular Arthritis

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Melatonin Abates TMJOA Chronic Pain by MT₂R in Trigeminal Ganglion Neurons