Chronic nicotine increases alcohol self-administration in adult male Wistar rats

Montanari, Christian; Secci, Maria E.; Driskell, Ashlyn; McDonald, Katherine O.; Schratz, Connor L.; Gilpin, Nicholas W.

doi:10.1007/s00213-020-05669-8

Cited by 10 publications

(6 citation statements)

References 54 publications

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“…After reaching adulthood (PND 61), using the SA paradigm, we studied alcohol-related behaviors in mice, which reflect various AUD components in humans [ 72 ]. Although food and/or water deprivation is a common approach to facilitate operant learning in the SA procedure, non-deprived SA protocols are increasingly common [ 73 , 74 , 75 , 76 ]. In Experiment 1, when the SA procedure started, mice only had access to food for 1 h. Because our study aimed to differentiate between the effects of continuous HFD and intermittent bingeing on HFD, limiting the food access during the SA presented apparent disadvantages.…”

Section: Discussionmentioning

confidence: 99%

Pairing Binge Drinking and a High-Fat Diet in Adolescence Modulates the Inflammatory Effects of Subsequent Alcohol Consumption in Mice

González-Portilla

Montagud‐Romero

Navarrete

et al. 2021

IJMS

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Alcohol binge drinking (BD) and poor nutritional habits are two frequent behaviors among many adolescents that alter gut microbiota in a pro-inflammatory direction. Dysbiotic changes in the gut microbiome are observed after alcohol and high-fat diet (HFD) consumption, even before obesity onset. In this study, we investigate the neuroinflammatory response of adolescent BD when combined with a continuous or intermittent HFD and its effects on adult ethanol consumption by using a self-administration (SA) paradigm in mice. The inflammatory biomarkers IL-6 and CX3CL1 were measured in the striatum 24 h after BD, 3 weeks later and after the ethanol (EtOH) SA. Adolescent BD increased alcohol consumption in the oral SA and caused a greater motivation to seek the substance. Likewise, mice with intermittent access to HFD exhibited higher EtOH consumption, while the opposite effect was found in mice with continuous HFD access. Biochemical analyses showed that after BD and three weeks later, striatal levels of IL-6 and CX3CL1 were increased. In addition, in saline-treated mice, CX3CL1 was increased after continuous access to HFD. After oral SA procedure, striatal IL-6 was increased only in animals exposed to BD and HFD. In addition, striatal CX3CL1 levels were increased in all BD- and HFD-exposed groups. Overall, our findings show that adolescent BD and intermittent HFD increase adult alcohol intake and point to neuroinflammation as an important mechanism modulating this interaction.

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Section: Discussionmentioning

confidence: 99%

Pairing Binge Drinking and a High-Fat Diet in Adolescence Modulates the Inflammatory Effects of Subsequent Alcohol Consumption in Mice

González-Portilla

Montagud‐Romero

Navarrete

et al. 2021

IJMS

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“…Since its original publication, ezTrack has grown in popularity, owing to many of its key features (Bitzenhofer, Pöpplau, Chini, Marquardt, & Hanganu‐Opatz, 2021; Bourdenx et al., 2021; Kesner et al., 2021; McCauley et al., 2020; Montanari et al., 2021; Rajbhandari et al., 2021; Shuman et al., 2020; Zeidler, Hoffmann, & Krook‐Magnuson, 2020). It is free, easy to use, produces highly reliable and accurate results, contains a range of interactive data exploration/visualization tools, has a suite of functions to refine videos, and has no operating system or hardware requirements.…”

Section: Introductionmentioning

confidence: 99%

ezTrack—A Step‐by‐Step Guide to Behavior Tracking

et al. 2021

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Tracking animal behavior by video is one of the most common tasks in neuroscience. Previously, we have validated ezTrack, a free, flexible, and easy-touse software for the analysis of animal behavior. ezTrack's Location Tracking Module can be used for the positional analysis of an individual animal and is applicable to a wide range of behavioral tasks. Separately, ezTrack's Freeze Analysis Module is designed for the analysis of defensive freezing behavior. ezTrack supports a range of desirable tools, including options for cropping and masking portions of the field of view, defining regions of interest, producing summary data for specified portions of time, algorithms to remove the influence of electrophysiology cables and other tethers, batch processing of multiple videos, and video down-sampling. Moreover, ezTrack produces a range of interactive plots and visualizations to promote users' confidence in their results. In this protocols paper, we provide step-by-step instructions for the use of ezTrack, from tips for recording behavior to instructions for using the software for video analysis.

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“…The enhancing effect of nicotine was only present while nicotine was onboard, as drug intake fell to control levels when the animals' received injections of saline or self-administered saline. The ability of nicotine to serve as a primary reinforcer, while also promoting seeking of reinforcers unassociated with nicotine itself has been well established 8,10,30,31 , and these effects apply to non-drug stimuli and drugs of abuse 12,18 . The present results extend this effect to cannabinoids, further increasing understanding of the impact of nicotine on drug use.…”

Section: Discussionmentioning

confidence: 99%

“…Nicotine is the primary reinforcing compound in tobacco products and it exhibits significant reinforcement enhancing effects by increasing behavioral responding for stimuli that are not associated with acquiring nicotine itself [7][8][9][10] . Nicotine enhances responding for non-drug stimuli, and promotes intake of other drugs of abuse such as cocaine, methamphetamine, and ethanol [11][12][13][14][15][16][17][18][19] . Despite these effects on other commonly abused substances, there is a substantial gap in knowledge concerning the effects of nicotine on cannabinoid reinforcement.…”

Section: Introductionmentioning

confidence: 99%

Nicotine enhances intravenous self-administration of cannabinoids in adult rats

Stringfield

Sanders

Suppo

et al. 2022

Preprint

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Introduction: Nicotine and cannabis are commonly used together, yet few studies have investigated the effects of concurrent administration of both drugs. Nicotine exhibits reinforcement enhancing effects by promoting the reinforcing properties of stimuli including other drugs. As many studies of this effect have used non-contingent nicotine, we implemented a dual-self-administration model where rats are given simultaneous access to two drugs and choose which to self-administer throughout a session. Here, we investigated the effect of self-administered or non-contingently delivered nicotine on cannabinoid self-administration. Methods: Adult male rats were allowed to self-administer the synthetic cannabinoid WIN 55,212-2 (WIN) intravenously, with or without subcutaneous nicotine injections before each session. A separate group of animals were allowed to self-administer WIN, nicotine, or saline using a dual-catheter procedure, where each solution was infused independently and associated with a separate operant response. A third group of male and female rats were allowed to self-administer delta-9-tetrahydrocannabinol (THC) with or without pre-session injections of nicotine. Results: Nicotine injections increased self-administration of WIN and THC. During dual self-administration, nicotine availability increased saline and WIN infusions but nicotine intake was not changed by WIN or saline availability. Rats preferred nicotine over saline, but preferred nicotine and WIN equally when both were available. The effect of nicotine on cannabinoid self-administration was acute and reversible when nicotine was no longer present. Conclusions: These results expand our understanding of the ability of nicotine to enhance reinforcement of other drugs of abuse and suggest that co-use of nicotine and cannabinoids promotes cannabinoid use in excess of what would be taken alone. Implications: This study utilizes a dual intravenous self-administration model to investigate the ability of nicotine to enhance cannabinoid intake. Our results demonstrate that the reinforcement enhancing properties of nicotine on drug use extend to include cannabinoids, but that this effect occurs specifically when nicotine is administered alongside the cannabinoid. Interestingly, cannabinoid use did not promote nicotine intake, suggesting this mechanism of reinforcement is specific to nicotine.

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Chronic nicotine increases alcohol self-administration in adult male Wistar rats

Cited by 10 publications

References 54 publications

Pairing Binge Drinking and a High-Fat Diet in Adolescence Modulates the Inflammatory Effects of Subsequent Alcohol Consumption in Mice

Pairing Binge Drinking and a High-Fat Diet in Adolescence Modulates the Inflammatory Effects of Subsequent Alcohol Consumption in Mice

ezTrack—A Step‐by‐Step Guide to Behavior Tracking

Nicotine enhances intravenous self-administration of cannabinoids in adult rats

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