Chronic nicotine exposure augments gustatory plasticity in<i>Caenorhabditis elegans:</i>involvement of dopamine signaling

Matsuura, Tetsuya; Urushihata, Takuya

doi:10.1080/09168451.2014.980220

Cited by 8 publications

(21 citation statements)

References 42 publications

(74 reference statements)

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“…In the few studies conducted thus far, several molecular targets have been identified in various behavioral paradigms across SOA classes using C. elegans (Engleman et al, 2016). Thus far, it appears that genes involved in monoamine neurotransmission mediate at least some behaviors induced by each SOA (Bettinger and McIntire, 2004; Lee et al, 2009; Ward et al, 2009; Musselman et al, 2012; Sellings et al, 2013; Topper et al, 2014; Matsuura and Urushihata, 2015). In particular, mutation of the gene coding for tyrosine hydroxylase ( cat-2 ) reduced or inhibited SOA-induced behaviors for each SOA of abuse (Bettinger and McIntire, 2004; Musselman et al, 2012; Matsuura and Urushihata, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Thus far, it appears that genes involved in monoamine neurotransmission mediate at least some behaviors induced by each SOA (Bettinger and McIntire, 2004; Lee et al, 2009; Ward et al, 2009; Musselman et al, 2012; Sellings et al, 2013; Topper et al, 2014; Matsuura and Urushihata, 2015). In particular, mutation of the gene coding for tyrosine hydroxylase ( cat-2 ) reduced or inhibited SOA-induced behaviors for each SOA of abuse (Bettinger and McIntire, 2004; Musselman et al, 2012; Matsuura and Urushihata, 2015). It is widely thought that the dopamine neurotransmitter system plays an important role in drug abuse (Koob, 1992; Koob and Volkow, 2010) and all of the SOAs discussed here have effects on dopamine neurotransmission.…”

Section: Discussionmentioning

confidence: 99%

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Caenorhabditis elegans Show Preference for Stimulants and Potential as a Model Organism for Medications Screening

et al. 2018

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The nematode Caenorhabditis elegans (C. elegans) is a popular invertebrate model organism to study neurobiological disease states. This is due in part to the intricate mapping of all neurons and synapses of the entire animal, the wide availability of mutant strains, and the genetic and molecular tools that can be used to manipulate the genome and gene expression. We have shown that, C. elegans develops a conditioned preference for cues that had previously been paired with either cocaine or methamphetamine exposure that is dependent on dopamine neurotransmission, similar to findings using place conditioning with rats and mice. In the current study, we show C. elegans also display a preference for, and self-exposure to, cocaine and nicotine. This substance of abuse (SOA) preference response can be selectively blocked by pretreatment with naltrexone and is consistent with the recent discovery of an opioid receptor system in C. elegans. In addition, pre-exposure to the smoking cessation treatment varenicline also inhibits self-exposure to nicotine. Exposure to concentrations of treatments that inhibit SOA preference/self-exposure did not induce any significant inhibition of locomotor activity or affect food or benzaldehyde chemotaxis. These data provide predictive validity for the development of high-throughput C. elegans behavioral medication screens. These screens could enable fast and accurate generation of data to identify compounds that may be effective in treating human addiction. The successful development and validation of such models would introduce powerful and novel tools in the search for new pharmacological treatments for substance use disorders, and provide a platform to study the mechanisms that underlie addictions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Caenorhabditis elegans Show Preference for Stimulants and Potential as a Model Organism for Medications Screening

et al. 2018

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“…In reviewing the molecular targets identified in the behavioral paradigms as described in Table 1, monoamine neurotransmission-associated genes are involved in mediating at least some behaviors induced by each drug. 20,31,41,68,75–77 In particular, within this classification, mutation of the tyrosine hydroxylase gene cat-2 resulted in reductions in drug-induced behaviors for each drug of abuse. 20,76,77 Although this type of mutation is lethal in rodents, 78 drugs of abuse have long been known to function, at least in part, through the dopamine neurotransmitter system 3,27 and all of the addictive drugs examined here have direct or indirect effect on dopamine systems.…”

Section: Convergent Mechanisms Of Drugs Of Abuse In C Elegansmentioning

confidence: 99%

Caenorhabditis elegans as a Model to Study the Molecular and Genetic Mechanisms of Drug Addiction

Engleman

Katner

Neal-Beliveau

2016

Progress in Molecular Biology and Translational Science

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Drug addiction takes a massive toll on society. Novel animal models are needed to test new treatments and understand the basic mechanisms underlying addiction. Rodent models have identified the neurocircuitry involved in addictive behavior and indicate that rodents possess some of the same neurobiologic mechanisms that mediate addiction in humans. Recent studies indicate that addiction is mechanistically and phylogenetically ancient and many mechanisms that underlie human addiction are also present in invertebrates. The nematode Caenorhabditis elegans has conserved neurobiologic systems with powerful molecular and genetic tools and a rapid rate of development that enables cost-effective translational discovery. Emerging evidence suggests that C. elegans is an excellent model to identify molecular mechanisms that mediate drug-induced behavior and potential targets for medications development for various addictive compounds. C. elegans emit many behaviors that can be easily quantitated including some that involve interactions with the environment. Ethanol (EtOH) is the best-studied drug-of-abuse in C. elegans and at least 50 different genes/targets have been identified as mediating EtOH’s effects and polymorphisms in some orthologs in humans are associated with alcohol use disorders. C. elegans has also been shown to display dopamine and cholinergic system–dependent attraction to nicotine and demonstrate preference for cues previously associated with nicotine. Cocaine and methamphetamine have been found to produce dopamine-dependent reward-like behaviors in C. elegans. These behavioral tests in combination with genetic/molecular manipulations have led to the identification of dozens of target genes/systems in C. elegans that mediate drug effects. The one target/gene identified as essential for drug-induced behavioral responses across all drugs of abuse was the cat-2 gene coding for tyrosine hydroxylase, which is consistent with the role of dopamine neurotransmission in human addiction. Overall, C. elegans can be used to model aspects of drug addiction and identify systems and molecular mechanisms that mediate drug effects. The findings are surprisingly consistent with analogous findings in higher-level organisms. Further, model refinement is warranted to improve model validity and increase utility for medications development.

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“…The experimental protocol for salt chemotaxis assays was reported previously. 22) Briefly, salt chemotaxis assays (gustatory plasticity assays for NaCl) used tissue culture dishes (9 cm diameter) containing 1 mM CaCl 2 , 1 mM MgSO 4 , 5 mM KH 2 PO 4 (pH 6.0), and 17 g/L agar (without NaCl). To establish a concentration gradient of NaCl, 100 mM NaCl (7 μL) was spotted on the surface of an assay plate (NaCl location: Fig.…”

Section: Measurement Of Locomotor Activitymentioning

confidence: 99%

Inhibitory effects of caffeine on gustatory plasticity in the nematode Caenorhabditis elegans

Urushihata

Takuwa

Higuchi

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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The effects of caffeine on salt chemotaxis learning were investigated using the nematode Caenorhabditis elegans. To estimate the degree of salt chemotaxis learning, nematodes were placed in a mixed solution of NaCl and caffeine, and then the chemotaxis index of NaCl was obtained from the nematodes placed on agar medium after pre-exposure to caffeine concentrations of 0.01, 0.1, 0.3, and 1.0%. Locomotor activity and preference behavior for caffeine were also estimated under these caffeine conditions. Nematodes pre-exposed to 0.3% caffeine showed inhibition of salt chemotaxis learning. Additional experiments indicated that nematodes showed a preference response to the middle concentration of caffeine (0.1%), with preference behavior declining in the 0.3% caffeine condition. Stable locomotor activity was observed under 0.01-0.3% caffeine conditions. These results suggest that salt chemotaxis learning with 0.3% caffeine is useful for investigating the effects of caffeine on learning in nematodes.

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Chronic nicotine exposure augments gustatory plasticity inCaenorhabditis elegans:involvement of dopamine signaling

Cited by 8 publications

References 42 publications

Caenorhabditis elegans Show Preference for Stimulants and Potential as a Model Organism for Medications Screening

Caenorhabditis elegans Show Preference for Stimulants and Potential as a Model Organism for Medications Screening

Caenorhabditis elegans as a Model to Study the Molecular and Genetic Mechanisms of Drug Addiction

Inhibitory effects of caffeine on gustatory plasticity in the nematode Caenorhabditis elegans

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