Chronic Iron Overload Restrains the Benefits of Aerobic Exercise to the Vasculature

Rossi, Emilly M.; Ávila, Renata Andrade; Carneiro, Maria Tereza Weitzel Dias; Almenara, Camila Cruz Pereira; Santos, Leonardo dos

doi:10.1007/s12011-020-02078-y

Cited by 6 publications

(7 citation statements)

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“…An in vitro study on the effects of high iron concentrations on isolated rat cardiac muscle showed that OH • and H 2 O 2 are the most commonly generated ROS, and that pre-incubation with DMSO and catalase, but not with a SOD mimetic, was able to prevent the contractile dysfunction induced by iron overload (Ávila et al, 2016). In addition, Rossi et al (2020) demonstrated that aerobic exercise also increased the expression of catalase in artery samples from rats previously subjected to iron overload, but no effect was noted on SOD. Taylor et al (2003) described how the hearts of exercised animals were more tolerant to the effects of hydrogen peroxide.…”

Section: Discussionmentioning

confidence: 95%

“…Although iron overload was associated with cardiac iron deposition and (Brissot et al, 2018;Coates, 2014;Harada et al, 1998) and animal models (Bertoli et al, 2018;Fidelis et al, 2020;Rossi et al, 2020). Transferrin saturation also exceeded 20−50%, the limit commonly used to diagnose iron overload (Elsayed et al, 2016;Sebastiani et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…After adaptation, to assess baseline aerobic capacity the rats were submitted to a maximum running speed (MRS) test, during which the speed was increased by 3 m/min every 3 min until fatigue. Fatigue was defined as the moment when the rat was no longer able to follow the treadmill speed and the MRS was determined (Rossi et al, 2020).…”

Section: Experimental Designmentioning

confidence: 99%

“…The Fe group was then divided randomly into sedentary (Fe) or exercised (Fe+Ex) iron-overloaded rats, and the Ct rats were kept as sedentary. Moderate-intensity aerobic training was performed on a motor-driven treadmill for 8 weeks (Rossi et al, 2020…”

Section: Experimental Designmentioning

confidence: 99%

“…Image acquisition and immunoblot quantification were performed using ChemiDoc (Bio-Rad Laboratories; RRID:SCR_019037). Transference efficiency and protein normalization were done by the total protein content fluorescent detection of tryptophan residues for each sample (Rossi et al, 2020;Sukumaran et al, 2017).…”

Section: Western Blotting Analysismentioning

confidence: 99%

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Moderate‐intensity aerobic training reduces cardiac damage attributable to experimental iron overload in rats

Ávila

Rossi

Carvalho

et al. 2021

Experimental Physiology

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Iron is an essential micronutrient for several life processes, but its excess can damage organs owing to oxidative stress, with cardiomyopathy being the leading cause of death in iron-overloaded patients. Although exercise has long been considered as a cardioprotective tool, its effects on iron overload are not known. This study was designed to investigate the effects of moderate-intensity aerobic training in rats previously submitted to chronic iron overload. Wistar rats received i.p. injections of iron dextran (100 mg/kg, 5 days/week for 4 weeks); thereafter, the rats were kept sedentary or exercised (60 min/day, progressive aerobic training, 60-70% of maximal speed, 5 days/week on a treadmill) for 8 weeks. At the end of the experimental period, haemodynamics were recorded and blood samples, livers and hearts harvested. Myocardial mechanics of papillary muscles were assessed in vitro, and cardiac remodelling was evaluated by histology and immunoblotting. Iron overload led to liver iron deposition, liver fibrosis and increased serum alanine aminotransferase and aspartate aminotransferase. Moreover, cardiac iron accumulation was accompanied by impaired myocardial mechanics, increased cardiac collagen type I and lipid peroxidation (TBARS), and release of creatine phosphokinase-MB to the serum. Although exercise did not influence iron levels, tissue injury markers were significantly reduced. Likewise, myocardial contractility and inotropic responsiveness were improved in exercised rats, in association with an increase in the endogenous antioxidant enzyme catalase.In conclusion, moderate-intensity aerobic exercise was associated with attenuated oxidative stress and cardiac damage in a rodent model of iron overload, thereby suggesting its potential role as a non-pharmacological adjuvant therapy for ironoverload cardiomyopathy.

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Section: Discussionmentioning

confidence: 95%