Chronic inhibition of chemokine receptor CXCR2 attenuates cardiac remodeling and dysfunction in spontaneously hypertensive rats

Selective blocking of CXCR2 prevents and reverses atrial fibrillation in spontaneously hypertensive rats

“…Blood pressure was measured from the age of 1 month and every month thereafter using a tail-cuff system (BP-2010A; Softron, Tokyo, Japan) as described previously. 14…”

“…The mRNA levels of CXCL1, CXCR2, α-smooth muscle actin (α-SMA), collagen I, collagen III, IL-1β, IL-6 and TNF-α were analysed using a PCR thermocycler (S1000 Thermal Cycler; Bio-Rad, Hercules, CA, USA) as described previously. 14 The values were normalized to those of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Primer sequences were provided in Table S1.…”

“…In contrast, genetic ablation or pharmacological blockage of CXCR2 reduces these effects in mice and spontaneously hypertensive rats (SHRs). [12][13][14] However, whether CXCR2 has a pro-arrhythmic role and whether blocking CXCR2 could be a new approach for the prevention and treatment of AF remain unknown.…”

Deficiency of the Immunoproteasome LMP10 Subunit Attenuates Angiotensin II-Induced Cardiac Hypertrophic Remodeling via Autophagic Degradation of gp130 and IGF1R

“…Male mice (8-10 weeks old) were infused with saline or Ang II (Sigma-Aldrich, St. Louis, MO, United States) at a dose of 1,000 ng/kg/min using osmotic mini-pumps (Alzet, Cupertino, CA, United States) as described in our previous work . Blood pressure was measured in conscious mice by using a tail-cuff system (BP2010A; Softron, Tokyo, Japan) after Ang II infusion (Wang et al, 2016;Zhang et al, 2019). All animals were kept in a pathogen-free facility at Capital Medical University.…”

“…Total RNA was extracted from heart tissue with TRIzol (Invitrogen, Carlsbad, CA, United States) and reverse transcribed. cDNA (1-2 µg) was used for PCR amplification with gene-specific primers for LMP10, B-type natriuretic peptide (BNP), β-myosin heavy chain (β-MHC), interleukin (IL)-1β, IL-6, monocyte chemoattractant protein 1 (MCP-1), collagen I, and collagen III as described previously Zhang et al, 2019). The amount of detected mRNA was normalized to the amount of endogenous GAPDH control.…”