Chronic High-Fat Feeding Affects the Mesenchymal Cell Population Expanded From Adipose Tissue but Not Cardiac Atria

Perbellini, Filippo; Gomes, Renata S. M.; Vieira, Sílvia; Buchanan, Dougal; Malandraki-Miller, Sophia; Bruyneel, Arne A.N.; Fialho, Maria da Luz Sousa; Ball, Vicky; Clarke, Kieran; Faggian, Giuseppe; Carr, Carolyn A.

doi:10.5966/sctm.2015-0024

Cited by 8 publications

(5 citation statements)

References 48 publications

(57 reference statements)

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“…CPCs will experience a shift in substrate availability following transplantation in vivo (Figure 3 ), being transferred from the culture medium, which contains about 5–25 mM glucose (depending on the culture protocol) and no FFA, to substrates in plasma that vary substantially. Glucose levels in mice have been measured at between ~3.4 and 9.6 mM (2.8–7.5 mM in rats) ( 162 , 163 ) and ~0.18–0.6 mM FFA ( 164 ). In humans, healthy plasma glucose levels are around 5 mM and FFA 0.5 mM ( 165 ).…”

Section: Why Study Stem Cell Metabolismmentioning

confidence: 99%

Changing Metabolism in Differentiating Cardiac Progenitor Cells—Can Stem Cells Become Metabolically Flexible Cardiomyocytes?

Malandraki-Miller

Lopez

Al-Siddiqi

et al. 2018

Front. Cardiovasc. Med.

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The heart is a metabolic omnivore and the adult heart selects the substrate best suited for each circumstance, with fatty acid oxidation preferred in order to fulfill the high energy demand of the contracting myocardium. The fetal heart exists in an hypoxic environment and obtains the bulk of its energy via glycolysis. After birth, the “fetal switch” to oxidative metabolism of glucose and fatty acids has been linked to the loss of the regenerative phenotype. Various stem cell types have been used in differentiation studies, but most are cultured in high glucose media. This does not change in the majority of cardiac differentiation protocols. Despite the fact that metabolic state affects marker expression and cellular function and activity, the substrate composition is currently being overlooked. In this review we discuss changes in cardiac metabolism during development, the various protocols used to differentiate progenitor cells to cardiomyocytes, what is known about stem cell metabolism and how consideration of metabolism can contribute toward maturation of stem cell-derived cardiomyocytes.

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Section: Why Study Stem Cell Metabolismmentioning

confidence: 99%

Changing Metabolism in Differentiating Cardiac Progenitor Cells—Can Stem Cells Become Metabolically Flexible Cardiomyocytes?

Malandraki-Miller

Lopez

Al-Siddiqi

et al. 2018

Front. Cardiovasc. Med.

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“…Of note, due to the different anatomic abundance and location of adipose tissue hASCs are still under ongoing comparative study to establish if they have the same cell phenotype independent of the adipose tissue origin [ 21 , 29 , 30 ]. Thus, elucidating this issue could have a deep impact on stem cell replacement therapeutic approaches since it will allow the isolation of hASCs from the adipose tissue that requires less invasive surgery with low economic impact in public health.…”

Section: Introductionmentioning

confidence: 99%

Ex-Vivo Tissues Engineering Modeling for Reconstructive Surgery Using Human Adult Adipose Stem Cells and Polymeric Nanostructured Matrix

et al. 2016

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The major challenge for stem cell translation regenerative medicine is the regeneration of damaged tissues by creating biological substitutes capable of recapitulating the missing function in the recipient host. Therefore, the current paradigm of tissue engineering strategies is the combination of a selected stem cell type, based on their capability to differentiate toward committed cell lineages, and a biomaterial, that, due to own characteristics (e.g., chemical, electric, mechanical property, nano-topography, and nanostructured molecular components), could serve as active scaffold to generate a bio-hybrid tissue/organ. Thus, effort has been made on the generation of in vitro tissue engineering modeling. Here, we present an in vitro model where human adipose stem cells isolated from lipoaspirate adipose tissue and breast adipose tissue, cultured on polymeric INTEGRA® Meshed Bilayer Wound Matrix (selected based on conventional clinical applications) are evaluated for their potential application for reconstructive surgery toward bone and adipose tissue. We demonstrated that human adipose stem cells isolated from lipoaspirate and breast tissue have similar stemness properties and are suitable for tissue engineering applications. Finally, the overall results highlighted lipoaspirate adipose tissue as a good source for the generation of adult adipose stem cells.

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“…Another plausible explanation is that the activation of Ddr2 in other joint tissues may also play a role in the destruction of articular cartilage. Data from several studies demonstrate DDR2 expression in bone tissue, 35,36 and that DDR2 activation causes osteoblast differentiation, which, in turn, may affect the remodeling and stability of the subchondral bone. 37,38 Another good example of a whole joint treatment approach is the role of transforming growth factor b1 signaling in the pathogenesis of OA.…”

Section: Discussionmentioning

confidence: 99%

Discoidin Domain Receptor 2 as a Potential Therapeutic Target for Development of Disease-Modifying Osteoarthritis Drugs

Manning

Chickmagalur

et al. 2016

The American Journal of Pathology

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Osteoarthritis (OA) is the most common form of arthritis disorders, but the identification of therapeutic targets to effectively prevent OA has been increasingly difficult. The goal of this investigation is to provide experimental evidence that discoidin domain receptor 2 (DDR2) may be an ideal target for the development of disease-modifying OA drugs. Ddr2 was conditionally deleted from articular cartilage of adult mouse knee joints. Aggrecan-CreERT2;floxed Ddr2 mice, which were generated by crossing Aggrecan-CreERT2 mice with floxed Ddr2 mice, then received tamoxifen injections at the age of 8 weeks. The mice were then subjected to destabilization of the medial meniscus (DMM) surgery. At 8 and 16 weeks after DMM, mice were euthanized for the collection of knee joints. In a separate experiment, Aggrecan-CreERT2;floxed Ddr2 mice were subjected to DMM at the age of 10 weeks. The mice then received tamoxifen injections at 8 weeks after DMM. The mice were euthanized for the collection of knee joints at 16 weeks after DMM. The progressive process of articular cartilage degeneration was significantly delayed in the knee joints of Ddr2-deficient mice in comparison to their control littermates. Articular cartilage damage in the knee joints of the mice was associated with increased expression profiles of both Ddr2 and matrix metalloproteinase 13. These findings suggest that DDR2 may be an ideal target for the development of disease-modifying OA drugs.

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Chronic High-Fat Feeding Affects the Mesenchymal Cell Population Expanded From Adipose Tissue but Not Cardiac Atria

Cited by 8 publications

References 48 publications

Changing Metabolism in Differentiating Cardiac Progenitor Cells—Can Stem Cells Become Metabolically Flexible Cardiomyocytes?

Changing Metabolism in Differentiating Cardiac Progenitor Cells—Can Stem Cells Become Metabolically Flexible Cardiomyocytes?

Ex-Vivo Tissues Engineering Modeling for Reconstructive Surgery Using Human Adult Adipose Stem Cells and Polymeric Nanostructured Matrix

Discoidin Domain Receptor 2 as a Potential Therapeutic Target for Development of Disease-Modifying Osteoarthritis Drugs

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