Chronic hepatosplenic abscesses in brucellosis. Clinico-therapeutic features and molecular diagnostic approach

Colmenero, Juan; Queipo-Ortuño, María Isabel; Reguera, J.M.; Suárez-Muñoz, Miguel Ángel; Martı́n-Carballino, Segundo; Morata, Pilar

doi:10.1016/s0732-8893(01)00344-3

Cited by 92 publications

(57 citation statements)

References 31 publications

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“…In the liver, Brucella infections induce hepatic lesions that can be both granulomatous and nongranulomatous (42,43). Histologically, granulomas show central necrosis, a polymorphic cellular infiltrate, few giant cells, and peripheral fibrosis (44). The most characteristic sign of inflammation in neurobrucellosis is meningitis, which is caused by invasion of the CNS by Brucella (45).…”

Section: Discussionmentioning

confidence: 99%

Lipoproteins, Not Lipopolysaccharide, Are the Key Mediators of the Proinflammatory Response Elicited by Heat-Killed Brucella abortus

Giambartolomei

Zwerdling

Cassataro

et al. 2004

The Journal of Immunology

148

156

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Inflammation is a hallmark of brucellosis. Although Brucella abortus, one of the disease’s etiologic agents, possesses cytokine-stimulatory properties, the mechanism by which this bacterium triggers a proinflammatory response is not known. We examined the mechanism whereby heat-killed B. abortus (HKBA), as well as its LPS, induces production of inflammatory cytokines in monocytes/macrophages. Polymyxin B, a specific inhibitor of LPS activity, did not inhibit the production of TNF-α- and IL-6-induced HKBA in the human monocytic cell line THP-1. HKBA induced the production of these cytokines in peritoneal macrophages of both C3H/HeJ and C3H/HeN mice, whereas B. abortus LPS only stimulated cells from C3H/HeN mice. Anti-TLR2 Ab, but not anti-TLR4 Ab, blocked HKBA-mediated TNF-α and IL-6 production in THP-1 cells. Because bacterial lipoproteins, a TLR2 ligand, have potent inherent stimulatory properties, we investigated the capacity of two B. abortus lipoproteins, outer membrane protein 19 (Omp19) and Omp16, to elicit a proinflammatory response. Lipidated (L)-Omp16 and L-Omp19, but not their unlipidated forms, induced the secretion of TNF-α, IL-6, IL-10, and IL-12 in a time- and dose-dependent fashion. Preincubation of THP-1 cells with anti-TLR2 Ab blocked L-Omp19-mediated TNF-α and IL-6 production. Together, these results entail a mechanism whereby B. abortus can stimulate cells from the innate immune system and induce cytokine-mediated inflammation in brucellosis. We submit that LPS is not the cause of inflammation in brucellosis; rather, lipoproteins of this organism trigger the production of proinflammatory cytokines, and TLR2 is involved in this process.

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Section: Discussionmentioning

confidence: 99%

Lipoproteins, Not Lipopolysaccharide, Are the Key Mediators of the Proinflammatory Response Elicited by Heat-Killed Brucella abortus

Giambartolomei

Zwerdling

Cassataro

et al. 2004

The Journal of Immunology

148

156

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“…On the other side, the RR is the output of the system, regulated by the HK, and effecting the cellular response. External signals modulate the activity of the HK, which autophosphorylates at a particular histidine residue, creating a high-energy phosphoryl group that , 10 we are particularly interested in two-component systems that could participate in an adaptive response to such environmental signal 11 , 12 .…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Snapshots of Conformational Changes Shed Light into the NtrX Receiver Domain Signal Transduction Mechanism

Fernández

Otero

Klinke

et al. 2015

Journal of Molecular Biology

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“…7 Infection occurs through direct or indirect contact with the primary source (the animal) via the skin or mucous membrane or ingestion of contaminated products, especially fresh dairy products. 7,8 Human brucellosis can have a variety of clinical manifestations, and the diagnosis may be easily missed unless the clinician is aware of a history of raw milk ingestion, exposure to animals, or travel to an area in which the disease is enzootic. 9 Our patient had a history of consuming unpasteurized cheese product.…”

Section: Discussionmentioning

confidence: 99%

“…in blood or any other body fluid or tissue sample, or the presence of a compatible clinical picture together with the demonstration of specific antibodies at significant titers or seroconversion. 8 US and CT are sensitive methods for diagnosing hepatic abscesses. 10 There are several characteristic imaging features of brucellar hepatic abscesses.…”

Section: Discussionmentioning

confidence: 99%

Acute Abdomen Caused by Brucellar Hepatic Abscess

Koca

Barut

Koca

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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Abstract. Brucellosis, a zoonosis that is common worldwide, is endemic in many countries, primarily those of the Mediterranean region (including Turkey). Human brucellosis is a systemic infection with a wide clinical spectrum. Although hepatic involvement is very common during the course of chronic brucellosis, hepatic abscess is a very rare complication of Brucella spp. infection. We present a case of hepatic abscess caused by Brucella melitensis, which resembled the clinical presentation of surgical acute abdomen.

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Chronic hepatosplenic abscesses in brucellosis. Clinico-therapeutic features and molecular diagnostic approach

Cited by 92 publications

References 31 publications

Lipoproteins, Not Lipopolysaccharide, Are the Key Mediators of the Proinflammatory Response Elicited by Heat-Killed Brucella abortus

Lipoproteins, Not Lipopolysaccharide, Are the Key Mediators of the Proinflammatory Response Elicited by Heat-Killed Brucella abortus

Snapshots of Conformational Changes Shed Light into the NtrX Receiver Domain Signal Transduction Mechanism

Acute Abdomen Caused by Brucellar Hepatic Abscess

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