Chronic exposure to field-realistic doses of imidacloprid resulted in biphasic negative effects on honey bee physiology

Kim, Sang-Hyun; Kim, Ju Hyeon; Cho, Susie; Lee, Do Eun; Clark, J. Marshall; Lee, Si Hyeock

doi:10.1016/j.ibmb.2022.103759

Cited by 10 publications

(3 citation statements)

References 70 publications

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“…On the day of population transfer (day 8), the cockroaches were briefly immobilized at low temperature (approximately 5 min at 5°C). A dot was drawn on the pronotum of each cockroach ( 30 ) with non-toxic water-based POSCA markers, often used to mark bees (e.g., see reference 31 ). The treated cockroaches were marked green, and untreated cockroaches were marked white.…”

Section: Methodsmentioning

confidence: 99%

Transmission of antimicrobial resistance in the gut microbiome of gregarious cockroaches: the importance of interaction between antibiotic exposed and non-exposed populations

Bogri,

Jensen,

Borchert

et al. 2024

mSystems

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Antimicrobial resistance (AMR) is a major global health concern, further complicated by its spread via the microbiome bacterial members. While mathematical models discuss AMR transmission through the symbiotic microbiome, experimental studies are scarce. Herein, we used a gregarious cockroach, Pycnoscelus surinamensis, as an in vivo animal model for AMR transmission investigations. We explored whether the effect of antimicrobial treatment is detectable with metagenomic sequencing, and whether AMR genes can be spread and established in unchallenged (not treated with antibiotics) individuals following contact with treated donors, and under various frequencies of interaction. Gut and soil substrate microbiomes were investigated by metagenomic sequencing for bacterial community composition and resistome profiling. We found that tetracycline treatment altered the treated gut microbiome by decreasing bacterial diversity and increasing the abundance of tetracycline resistance genes. Untreated cockroaches that interacted with treated donors also had elevated tetracycline resistance. The levels of resistance differed depending on the magnitude and frequency of donor transfer. Additionally, treated donors showed signs of microbiome recovery due to their interaction with the untreated ones. Similar patterns were also recorded in the soil substrate microbiomes. Our results shed light on how interacting microbiomes facilitate AMR gene transmission to previously unchallenged hosts, a dynamic influenced by the interaction frequencies, using an in vivo model to validate theoretical AMR transmission models. IMPORTANCE Antimicrobial resistance is a rising threat to human and animal health. The spread of resistance through the transmission of the symbiotic gut microbiome is of concern and has been explored in theoretical modeling studies. In this study, we employ gregarious insect populations to examine the emergence and transmission of antimicrobial resistance in vivo and validate modeling hypotheses. We find that antimicrobial treatment increases the levels of resistance in treated populations. Most importantly, we show that resistance increased in untreated populations after interacting with the treated ones. The level of resistance transmission was affected by the magnitude and frequency of population mixing. Our results highlight the importance of microbial transmission in the spread of antimicrobial resistance.

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Section: Methodsmentioning

confidence: 99%

Transmission of antimicrobial resistance in the gut microbiome of gregarious cockroaches: the importance of interaction between antibiotic exposed and non-exposed populations

Bogri,

Jensen,

Borchert

et al. 2024

mSystems

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“…8,9 Scholars have noted that the first-generation neonicotinoid pesticides, such as imidacloprid, exhibit greater subchronic toxicity toward pollinating insects, particularly honey bees. 10 As a result, the third-generation neonicotinoid insecticide dinotefuran has been optimized from the original version, resulting in significant differences from the previous two generations. Dinotefuran is the only insecticide that lacks chlorine atoms and aryl alkaloids; as a result, dinotefuran is more selective with remarkable insecticidal effects, exhibits low toxicity to mammals, and shows environmentally friendly characteristics.…”

Section: Introductionmentioning

confidence: 99%

“…The whitefly Bemisia tabaci (Hemiptera-Aleyrodidae) is a global pest damaging to numerous agricultural plants mainly by direct feeding and transmitting viruses. − To effective control of the whitefly, neonicotinoid insecticides were commonly applied worldwide owing to its high efficacy and strong systemic properties. − Neonicotinoid agents undergo the following distinct stages: first-generation chlorinated nicotinoid insecticides, second-generation thionicotine neonicotinoid agents, and third-generation furan-type nicotinic compounds. , Scholars have noted that the first-generation neonicotinoid pesticides, such as imidacloprid, exhibit greater subchronic toxicity toward pollinating insects, particularly honey bees . As a result, the third-generation neonicotinoid insecticide dinotefuran has been optimized from the original version, resulting in significant differences from the previous two generations.…”

Section: Introductionmentioning

confidence: 99%

Cytochrome P450 CYP6EM1 Underpins Dinotefuran Resistance in the Whitefly Bemisia tabaci

Huang,

Fu,

Yin

et al. 2024

J. Agric. Food Chem.

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Being a destructive pest worldwide, the whitefly Bemisia tabaci has evolved resistance to neonicotinoid insecticides. The third-generation neonicotinoid dinotefuran has commonly been applied to the control of the whitefly, but its underlying mechanism is currently unknown. On the base of our transcriptome data, here we aim to investigate whether the cytochrome P450 CYP6EM1 underlies dinotefuran resistance in the whitefly. Compared to the susceptible strain, the CYP6EM1 gene was found to be highly expressed in both laboratory and field dinotefuran-resistant populations. Upon exposure to dinotefuran, the mRNA levels of CYP6EM1 were increased. These results demonstrate the involvement of this gene in dinotefuran resistance. Loss and gain of functional studies in vivo were conducted through RNAi and transgenic Drosophila melanogaster assays, confirming the role of CYP6EM1 in conferring such resistance. In a metabolism assay in vitro, the CYP6EM1 protein could metabolize 28.11% of dinotefuran with a possible dinotefuran-dm-NNO metabolite via UPLC-QTOF/MS. Docking of dinotefuran to the CYP6EM1 protein showed a good binding affinity, with an energy of less than −6.0 kcal/mol. Overall, these results provide compelling evidence that CYP6EM1 plays a crucial role in the metabolic resistance of B. tabaci to dinotefuran. Our work provides new insights into the mechanism underlying neonicotinoid resistance and applied knowledge that can contribute to sustainable control of a global pest such as whitefly.

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A smartphone-integrated aptasensor for pesticide detection using gold-decorated microparticles

Ulloa-Gomez,

Waimin,

et al. 2024

Microchim Acta

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Chronic exposure to field-realistic doses of imidacloprid resulted in biphasic negative effects on honey bee physiology

Cited by 10 publications

References 70 publications

Transmission of antimicrobial resistance in the gut microbiome of gregarious cockroaches: the importance of interaction between antibiotic exposed and non-exposed populations

Transmission of antimicrobial resistance in the gut microbiome of gregarious cockroaches: the importance of interaction between antibiotic exposed and non-exposed populations

Cytochrome P450 CYP6EM1 Underpins Dinotefuran Resistance in the Whitefly Bemisia tabaci

A smartphone-integrated aptasensor for pesticide detection using gold-decorated microparticles

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