Chronic escalating-dose and acute binge cocaine treatments change the hippocampal cholinergic muscarinic system on drug presence and after withdrawal

Spelta, Lídia Emmanuela Wiazowski; Torres, Yuli Y.S.; Oliveira, Sarah C. W. S. E. Franco de; Yonamine, Maurı́cio; Bailey, Alexis; Camarini, Rosana; Garcia, Raphael Caio Tamborelli; Marcourakis, Tânia

doi:10.1016/j.taap.2022.116068

Cited by 2 publications

(4 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Animals were randomly separated into three groups. Groups 1 and 2 were kept conscious after [ 18 F]FDG injection and were injected intraperitoneally with saline 0.9% (image one—baseline) or cocaine (image 2; 20 mg/kg—[Gulley et al, 2003; Spelta et al, 2022]) 5 min after [ 18 F]FDG administration. Animals from group 1 ( n = 5, one animal died before starting the experiment) were then evaluated in the open field (a circular acrylic arena with a 40‐cm diameter and height) for 30 min, to verify cocaine‐induced locomotor activity.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

[¹⁸F]FDG brain uptake of C57Bl/6 male mice is affected by locomotor activity after cocaine use: A small animal positron emission tomography study

Spelta

Real

Buchpiguel

et al. 2022

J of Neuroscience Research

Self Cite

View full text Add to dashboard Cite

We verified if cocaine-induced peripheral activation might disrupt [ 18 F]FDG brain uptake after a cocaine challenge and suggested an optimal protocol to measure cocaine-induced brain metabolic alterations in mice. C57Bl/6 male mice were injected with [ 18 F]FDG and randomly separated into three groups. Groups 1 and 2 were kept conscious after [ 18 F]FDG administration and after 5 min received saline or cocaine (20 mg/kg). The animals in group 1 (n = 5) were then evaluated in the open field for 30 min and those from group 2 (n = 6) were kept alone in a home cage for the same period. Forty-five minutes after [ 18 F]FDG administration, images were acquired for 30 min. Group 3 (n = 6) was kept anesthetized and image acquisition started immediately after tracer injection, for 75 min. Saline (Day 1) or cocaine (Day 2) was injected 5 min after starting acquisition. Another set of animals (n = 5) were treated with cocaine every other day for 10 days or saline (n = 6) and were scanned with the dynamic protocol to verify its efficacy. [ 18 F]FDG uptake increased after cocaine administration when compared to baseline only in animals kept under anesthesia. No brain effect of cocaine was observed in animals submitted to the open field or kept in the home cage.The use of anesthesia is essential to visualize cocaine-induced changes in brain metabolism by [ 18 F]FDG PET, providing an interesting preclinical approach to investigate naïve subjects and enabling a bidirectional translational science approach for better understanding of cocaine use disorder.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Image Acquisition and Analysismentioning

confidence: 99%

[¹⁸F]FDG brain uptake of C57Bl/6 male mice is affected by locomotor activity after cocaine use: A small animal positron emission tomography study

Spelta

Real

Buchpiguel

et al. 2022

J of Neuroscience Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…No CANNELLA et al, 2017b;PORCHE'KIRKLAND et al, 2010;SPELTA et al, 2022). Foi observado em humanos que a administração aguda de cocaína aumenta o metabolismo de glicose nas regiões corticais pré-frontais envolvidas com mecanismos de atenção e expectativa de recompensas, bem como em regiões límbicas, como estriado, globo pálido, substância negra, e núcleo subtalâmico (LONDON et al, 1986).…”

Section: Discussionunclassified

“…Animals were randomly separated into three groups. Groups 1 and 2 were kept conscious after [ 18 F]FDG injection and were injected intraperitoneally with saline 0.9% (image one-baseline) or cocaine (image 2; 20 mg/kg-[Gulley et al, 2003;Spelta et al, 2022]) 5 min after[ 18 F]FDG administration. Animals from group 1 (n = 5, one animal died before starting the experiment) were then evaluated in the open field (a circular acrylic arena with a 40-cm diameter and height) for 30 min, to verify cocaine-induced locomotor activity.…”

mentioning

confidence: 99%

Modulação da atividade encefálica pelo canabidiol em camundongos tratados com cocaína

Spelta¹

View full text Add to dashboard Cite

Chronic escalating-dose and acute binge cocaine treatments change the hippocampal cholinergic muscarinic system on drug presence and after withdrawal

Cited by 2 publications

References 89 publications

[¹⁸F]FDG brain uptake of C57Bl/6 male mice is affected by locomotor activity after cocaine use: A small animal positron emission tomography study

[¹⁸F]FDG brain uptake of C57Bl/6 male mice is affected by locomotor activity after cocaine use: A small animal positron emission tomography study

Modulação da atividade encefálica pelo canabidiol em camundongos tratados com cocaína

Contact Info

Product

Resources

About

Chronic escalating-dose and acute binge cocaine treatments change the hippocampal cholinergic muscarinic system on drug presence and after withdrawal

Cited by 2 publications

References 89 publications

[18F]FDG brain uptake of C57Bl/6 male mice is affected by locomotor activity after cocaine use: A small animal positron emission tomography study

[18F]FDG brain uptake of C57Bl/6 male mice is affected by locomotor activity after cocaine use: A small animal positron emission tomography study

Modulação da atividade encefálica pelo canabidiol em camundongos tratados com cocaína

Contact Info

Product

Resources

About

[¹⁸F]FDG brain uptake of C57Bl/6 male mice is affected by locomotor activity after cocaine use: A small animal positron emission tomography study

[¹⁸F]FDG brain uptake of C57Bl/6 male mice is affected by locomotor activity after cocaine use: A small animal positron emission tomography study